Genetic basis and evolution of thermotolerance in the Saccharomyces yeast species

酵母菌耐热性的遗传基础和进化

• 批准号: 10433409
• 负责人: Justin C Fay
• 金额: $ 31.53万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-10 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10433409
• 关键词: Alleles; Biology; Characteristics; Chromosomes; Clustered Regularly Interspaced Short Palindromic Repeats; Complex; Dependence; Evolution; Genes; Genetic; Genome; Goals; Growth; High temperature of physical object; Hybrids; Individual; Investigation; Maps; Measures; Microbe; Mitotic Recombination; Organism; Phenotype; Polygenic Traits; Proteins; Proteomics; Research; Role; Saccharomyces; Saccharomyces cerevisiae; Sterility; Temperature; Testing; Time; Transgenic Organisms; Ursidae Family; Variant; Work; Yeasts; base; chromosome loss; fitness; genetic analysis; genome-wide; human pathogen; insight; melting; reproductive; species difference

PROJECT SUMMARY The goal of the project is to understand the genetic basis and evolution of thermotolerance in the Saccharomyces species. Whereas much has been learned about the causes of phenotypic variation within species, reproductive barriers have limited genetic analysis to closely related, inter-fertile species. Consequently, we know little about the genetic basis of substantial phenotypic differences that arise over longer time periods and the importance of evolutionary hotspots and historical contingency. To overcome this reproductive barrier we will use hybrid, proteomic and transgenic analysis of yeast species. In Aim 1 we will map thermotolerance in interspecific hybrids using reciprocal chromosome loss and CRISPR induced mitotic recombination. This will enable us to measure the combined effects of many genes, but also identify thermotolerance genes and determine: whether they evolved through the cumulative effects of multiple changes, and whether their effects depend on changes in other genes. In Aim 2 we will investigate divergence in protein thermal stability and whether it results in temperature dependent fitness effects not detected by mapping. In Aim 3 we will examine the reuse of thermotolerance genes during thermal divergence along independent lineages. Doing so will determine whether thermal divergence requires certain genes or can be achieved in different ways. Together, these aims will push the limits of interspecific genetic analysis in order to provide insight into the acquisition of a complex and broadly relevant phenotype that has taken millions of years to evolve.

项目摘要 该项目的目标是了解遗传基础和耐热性的演变， 酵母属。尽管人们对表型变异的原因已经了解得很多， 在物种内部，生殖障碍限制了对密切相关的、可互育的 物种因此，我们对表型差异的遗传基础知之甚少 以及进化热点和历史热点的重要性 意外事故为了克服这一生殖障碍，我们将使用杂交，蛋白质组学和转基因技术， 酵母种类分析在目标1中，我们将使用以下方法绘制种间杂种的耐热性图： 相互染色体丢失和CRISPR诱导的有丝分裂重组。这将使我们能够 测量许多基因的组合效应，但也要鉴定耐热基因并确定： 它们是否是通过多重变化的累积效应而进化的， 依赖于其他基因的变化。在目标2中，我们将研究蛋白质热稳定性的差异 以及它是否导致映射未检测到的依赖于温度的适应性效应。在目标3中， 将检查重复使用的耐热基因在热分化沿着独立 血统这样做将决定热发散是否需要某些基因，或者可以 以不同的方式实现。总之，这些目标将推动种间遗传分析的极限， 为了深入了解获得一个复杂的和广泛相关的表型， 花了数百万年才进化出来