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Isothermal amplification methods for point-of-care veterinary molecular diagnostics: proof of concept

用于现场兽医分子诊断的等温扩增方法：概念验证

基本信息

批准号：
10074248
负责人：
金额：
$ 6.37万
依托单位：
VETERINARY ISOTHERMAL MOLECULAR DIAGNOSTICS
依托单位国家：
英国
项目类别：
Grant for R&D
财政年份：
2023
资助国家：
英国
起止时间：
2023 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=10074248
关键词：
Isothermal amplification methods point care

项目摘要

This project seeks to make use of advances in isothermal amplification technologies and sample collection techniques, combined with temperature sensitive liposome technology to prove the concept of point-of-care (POC) molecular diagnostics capability in veterinary settings, including clinics, farms and abattoirs.Currently, molecular diagnostics for animals largely depends on PCR-based testing done at centralised specialist laboratories. The time between sample collection and molecular diagnosis is typically several days. This time lag could be reduced to less than 1 hour, while simultaneously preserving or improving on current standards of diagnostic sensitivity and test specificity. The innovation of this project includes uniting a set of relatively recent, but well-researched and established technological advances in a clever way that provides superior test performance with robustness and an easy-to-accomplish method.If the proof of concept is achieved, then this approach could be adapted to facilitate the detection of almost any pathogen of veterinary importance, very rapidly and at the POC. This would allow veterinarians to rapidly implement definitive therapy, for the benefit of animal welfare, antimicrobial resistance issues, owner satisfaction, and professional satisfaction in veterinary practice. The technology could find applications in diverse sectors including human health, meat hygiene, wildlife health monitoring and many more.

该项目旨在利用等温放大技术和样本采集技术的进步，结合温度敏感脂质体技术，在兽医环境中证明护理点(POC)分子诊断能力的概念，包括诊所、农场和屠宰场。目前，动物的分子诊断在很大程度上依赖于在中央专业实验室进行的基于PCR的测试。样本采集和分子诊断之间的时间通常是几天。这一时间延迟可以减少到不到1小时，同时保持或改进目前的诊断敏感性和测试特异性标准。该项目的创新包括以一种巧妙的方式结合了一系列相对较新的、经过充分研究和确立的技术进步，提供了卓越的测试性能和稳健性，以及一种易于实现的方法。如果概念验证成功，则该方法可以非常快速地在POC处适用于几乎任何兽医重要病原体的检测。这将使兽医能够为了动物福利、抗菌素耐药性问题、所有者满意和兽医实践中的专业满意度而迅速实施最终治疗。这项技术可以在不同的领域得到应用，包括人类健康、肉类卫生、野生动物健康监测等。