The Effect of Processed Grain Consumption on the Dentogingival Microbiome and Host Response in Periodontal Disease
加工谷物消费对牙周病牙龈微生物组和宿主反应的影响
基本信息
- 批准号:10442422
- 负责人:
- 金额:$ 5.26万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-01 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:16S ribosomal RNA sequencingActinomycesAddressAdoptionAffectAlveolar Bone LossAmylasesAutomobile DrivingBacteriaBiological AvailabilityBite ForceCarbohydratesCellsCommunitiesComplementary DNAConsumptionDataDebridementDevelopmentDietDigestionDiseaseDisease ProgressionE-CadherinEpithelial CellsExcisionFlow CytometryFluorescent in Situ HybridizationFoodForsythiaFusobacterium nucleatumGene ExpressionGenetic TranscriptionGingivaGrainGrowthHarvestHydrolysisImmuneImmune responseImmunityImmunohistochemistryIn VitroIncidenceInfectionInflammationInflammatory ResponseInvestigationMasticationMechanicsMicrobial BiofilmsMicrofluidicsModelingMusNutritionalOnset of illnessOralOral cavityOutcomePeriodontal DiseasesPeriodontal LigamentPhasePhysiologicalPopulationPorphyromonas gingivalisPredispositionPreventionProcessPropertyRNARoentgen RaysRoleSalivarySiteSoft DietStandardizationStarchStreptococcus gordoniiStreptococcus sanguisStructureSurfaceSystemTextureTissuesTranscriptTransmission Electron MicroscopyTreponema denticolaVirulenceWestern BlottingWheatbasebeta catenincarbohydrate metabolismdysbiosisimprovedin vivoin vivo Modellight microscopymandible/maxillamicroCTmicrobialmicrobial colonizationmicrobiomenovel strategiesnovel therapeutic interventionnovel therapeuticsoral microbial communityoral microbiomeparticlepathogenperiodontopathogenpolymicrobial biofilmresponsetomographytranscriptome sequencingwestern diet
项目摘要
PROJECT SUMMARY/ABSTRACT
Periodontal disease (PD) affects approximately 50% of the world population. Microbial dysbiosis and gingival
inflammation are well-recognized in PD. However, the role of diet on these parameters is minimally explored.
Increased PD incidence coincides with the adoption of the western diet that is characterized by consumption of
highly processed, refined grains. Grain processing alters both nutritional and structural properties of grains via
removal of the outer bran layer to isolate inner starch-rich components. This disrupts the integrity of the grain
structure, resulting in increased starch bioavailability and finer grain texture. Refined grain consumption is
associated with increased incidence of PD. However, the mechanisms by which refined grains increase PD
incidence are unexplored. Digestion commences in the oral phase via starch hydrolysis by salivary amylase and
mechanical digestion by mastication. Saccharides are made available to bacteria in the oral cavity via starch
hydrolysis by salivary amylase. Increased saccharide availability alters microbial carbohydrate metabolism,
driving microbial dysbiosis within dentogingival biofilm communities. Dentogingival surfaces provide an
opportunity for mature microbial biofilm formation. Excess biofilm formation drives dysbiosis as successional
colonization increases the representation of periodontal pathogens within the community. As such, mechanical
debridement is required to disrupt biofilm formation. Increased diet texture has been shown to decrease biofilm
accumulation. Moreover, increased masticatory forces by hard diets induce a protective inflammatory response
in gingival tissues, consistent with the role of the gingiva as a physiological barrier. As grain processing increases
starch bioavailability and its susceptibility to salivary amylase, this may increase saccharide availability to
bacteria in the oral cavity and encourage dysbiosis. Moreover, as grain processing disrupts the integrity of the
grain structure, such changes may reduce masticatory forces and thereby encourage excess microbial
colonization and reduce gingival barrier function. Therefore, I hypothesize that processed grains induce PD
progression by promoting microbial dysbiosis and/or by conferring reduced gingival barrier function via
local influences within the oral cavity. This proposal will address my hypothesis via two specific aims: 1)
Determine the impact of processed grains on development of multispecies dentogingival biofilms in vitro, and 2)
Determine the impact of processed grains on the dentogingival microbiome, periodontal immune response, and
gingival barrier function in vivo. These aims will be achieved utilizing a combination of in vitro and in vivo models
to recapitulate changes in the dentogingival microbiome and periodontal immunity in response to processed
grain consumption. Outcomes from this investigation will improve the understanding of the role of diet in PD
progression and will create avenues for the development of novel therapeutic applications that leverage the oral
microbiome for prevention of PD onset and progression.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Lea Maryam Sedghi其他文献
Lea Maryam Sedghi的其他文献
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{{ truncateString('Lea Maryam Sedghi', 18)}}的其他基金
The Effect of Processed Grain Consumption on the Dentogingival Microbiome and Host Response in Periodontal Disease
加工谷物消费对牙周病牙龈微生物组和宿主反应的影响
- 批准号:
10652427 - 财政年份:2021
- 资助金额:
$ 5.26万 - 项目类别:
The Effect of Processed Grain Consumption on the Dentogingival Microbiome and Host Response in Periodontal Disease
加工谷物消费对牙周病牙龈微生物组和宿主反应的影响
- 批准号:
10315269 - 财政年份:2021
- 资助金额:
$ 5.26万 - 项目类别:
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