Light Sheet Microscopy for Einstein Shared Facility

该提案寻求资金，为神经细胞购买Zeiss Light Sheet 7显微镜 罗丝·F·肯尼迪智力和技术中心的工程和成像核心(NCEI) 阿尔伯特·爱因斯坦医学院发展障碍研究(IDDR)。 近35年来，NCEI核心一直是爱因斯坦研究人员的宝贵共享设施 好几年了。在此期间，核心得到了肯尼迪国际复员方案中心的大力资金支持， 由NIH/NICHD中心拨款、神经科学系和学校资助。在最近 多年来，薄片成像方法的开发和使用显著增加，以 生物和生物医学研究，促进全面评估，否则不容易 获得。这为组织图案化、细胞化提供了前所未有的全球视角 架构、功能组合和远程连接，并可能导致意外 新的见解。光片显微镜设施的建立将把这项重要技术带到 爱因斯坦社区第一次，并遇到了什么是可以理解的重大意义 不断增长的需求。快速成像大量完整的组织和器官的能力将是 对于许多由NIH资助的iDDR中心和神经科学的研究人员来说， 医学、精神病学和行为科学系，以及其他。在所有情况下， 显微镜将在他们要求检查长距离神经元的研究中发挥变革性的作用 途径、体积测定、标记的特定细胞亚群计数和分布 复杂的混合单元型阵列。这些研究项目与神经发育有关 障碍、认知、成瘾、神经可塑性、癫痫、杀婴、动脉粥样硬化和血管 重塑、肥胖、压力和记忆。所提议的显微镜提供的能力 将是爱因斯坦研究人员的一项重要资产，允许进行其他方式不容易实现的研究， 提高生产力和增强新的生物医学见解。

This proposal seeks funding to purchase the Zeiss Light Sheet 7 microscope for the Neural Cell Engineering and Imaging Core (NCEI) of the Rose F Kennedy Center for Intellectual and Developmental Disabilities Research (IDDR) at the Albert Einstein College of Medicine (Einstein). The NCEI Core has served as a valuable shared facility for Einstein investigators for close to 35 years. During this the Core has received strong financial support from the Kennedy IDDR Center, funded by NIH/NICHD Center grants, the Department of Neuroscience, and the school. In recent years there has been marked increase in development and use of light sheet imaging approaches to biological and biomedical research, facilitating comprehensive evaluations not otherwise easily obtained. This provides unprecedented global perspective to tissue patterning, cellular architectonics, and functional ensembles and long-range connectivity, and can lead to unanticipated new insights. Establishment of a light sheet microscope facility will bring this important technology to the Einstein community for the first time, and meet what has been understandably a significantly growing demand. The ability to rapidly image large intact volumes of tissues and organs will be invaluable to numerous NIH-funded investigators of the IDDR center and of the Neuroscience, Medicine, Psychiatry and Behavioral Sciences departments, as well as others. In all cases, the microscope will be transformational in their studies that call for examination of long distance neuronal pathways, volume determinations, labeled specific cell sub-population counts and distributions, and complex mixed cell-type arrays. The research projects have relevance to neurodevelopmental disorders, cognition, addiction, neural plasticity, epilepsy, infanticide, atherosclerosis and vascular remodeling, obesity, and stress and memory. The capabilities provided by the proposed microscope would be a vital asset for Einstein researchers, permitting studies not otherwise readily possible, enhancing productivity and potentiating novel biomedical insights.