NEURAL CELL ENGINEERING AND IMAGING CORE

神经细胞工程和成像核心

• 批准号: 10669070
• 负责人: KOSTANTIN DOBRENIS
• 金额: $ 18.94万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-23 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669070
• 关键词: Address; Anatomy; Animal Experiments; Animals; Back; Biological Assay; Brain; Cell Line; Cell Separation; Cell model; Cells; Cognitive; Computer Workstations; Computer software; Consultations; Correlation Studies; Coupled; Custom; Data Analyses; Disease; Dissection; Electrophysiology (science); Embryo; Epigenetic Process; Equipment; Evaluation; Experimental Designs; Faculty; Funding; Gene Expression; Gene Targeting; Generations; Genetic; Goals; Grant; Human; Image; Image Analysis; In Situ; In Vitro; Individual; Injections; Intellectual and Developmental Disabilities Research Centers; Intellectual functioning disability; Investigation; Investments; Laboratories; Lasers; Leadership; Microscope; Microscopy; Modernization; Modification; Molecular; Monitor; Mutation; Names; Neuronal Differentiation; Neurons; Neurosciences; Organoids; Patients; Phenotype; Physiological; Preparation; Printing; Production; Productivity; Proteins; Proteomics; Protocols documentation; Publications; Quality Control; RNA; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Schedule; Services; Slice; Specimen; Structure; Surveys; Therapeutic Intervention; Time; Tissue Engineering; Tissue Model; Tissues; Training; United States National Institutes of Health; Vertebral column; Work; base; behavioral phenotyping; brain cell; cell preparation; cellular engineering; cellular imaging; clinical phenotype; college; computerized data processing; data acquisition; design; differentiation protocol; fetus cell; gene therapy; high end computer; high standard; image processing; image reconstruction; imaging software; in vivo; induced pluripotent stem cell; innovation; instrument; interest; mouse model; neural; neurogenomics; neuroimaging; optogenetics; reconstruction; synergism; therapeutic evaluation; tissue preparation; tissue/cell culture; tool; translational study; treatment strategy; ultra high resolution

PROJECT SUMMARY/ABSTRACT – (Core D: NCEI Core) The Neural Cell Engineering and Imaging Core (Core D, NCEI) has been a vital component of the Kennedy Center for 30 years, steadily growing in resources and user base, with 70 individuals from 50 IDDRC laboratories utilizing the Core in the last 3 years alone. The NCEI Core consists of 3 major divisions: The Cell and Tissue Engineering Subcore, with facilities and expertise for cell/tissue culture work including primary, embryonic, iPS cells and modern neural differentiation protocols. The Modern Microscopy Subcore, with instruments for tissue sectioning and imaging. And the Analysis and Graphics Subcore, which provides workstations with sophisticated software for deconvolution, quantitative 2D to 5D image analyses and reconstruction, custom software and macros, as well as graphics and printing services. To optimize use of these tools for IDDRC investigators and their trainees, NCEI leadership provides in-depth user consultation, emphasizes well-planned experimental design, and engages in vigilant monitoring of project progress. The Core’s value is extended linkage with expert advisors allowing studies on gene targeting, gene therapy, RNA constructs, optogenetics, electrophysiology, and human ES, iPS and fetal cells, including organoids. Productivity and innovation are also enhanced by interaction with other IDDRC Cores, coordinating, e.g., studies correlating brain cell and anatomic changes with behavioral phenotypes (AP), genetic or epigenetic evaluations on isolated cell subpopulations/single cells (NGEN), and translational studies on reprogrammed cells and iPSC-derived organoids from patients (HCP). Core resources also serve the P50 research project on KDM5C disease by providing imaging and quantitative analyses of changes in gene expression, analysis of neural subtypes and dendritic changes in mouse models, and generating iPS cells/organoids and differentiating neurons from patient samples carrying KDM5C mutations. The Core maintains high standards of quality control through scheduled internal evaluations and the annual user survey carried out by the ADM Core. Collectively, the NCEI Core represents a significant part of the scientific bridge between genetics and cognitive phenotypes and as such has a broad and significant impact on the research carried out by IDDRC investigators and their trainees focused on, and critical to, understanding and treating IDDs.

项目概要/摘要-（核心D：NCEI核心） 神经细胞工程和成像核心（核心D，NCEI）一直是肯尼迪的重要组成部分， 中心成立30年，资源和用户群稳步增长，拥有来自50个IDDRC的70名员工 仅在过去三年中，就有实验室使用了核心。NCEI核心由3个主要部门组成： 和组织工程分中心，拥有细胞/组织培养工作的设施和专业知识，包括初级， 胚胎、iPS细胞和现代神经分化方案。现代显微镜子核心， 用于组织切片和成像的仪器。以及分析和图形子核心，它提供 工作站，配备先进的软件，用于解卷积、定量2D至5D图像分析， 重建，定制软件和宏，以及图形和打印服务。优化使用 这些工具为IDDRC调查人员和他们的学员，NCEI领导提供了深入的用户咨询， 强调精心策划的实验设计，并对项目进度进行警惕的监控。的 核心的价值是与专家顾问的延伸联系，允许对基因靶向，基因治疗，RNA 构建体、光遗传学、电生理学和人ES、iPS和胎儿细胞，包括类器官。 生产力和创新也通过与其他IDDRC核心的互动来提高，例如， 将脑细胞和解剖学变化与行为表型（AP）、遗传或表观遗传相关的研究 对分离的细胞亚群/单细胞（NGEN）的评价，以及对重编程 细胞和iPSC衍生的类器官（HCP）。核心资源还服务于P50研究项目， 通过提供基因表达变化的成像和定量分析， 小鼠模型中的神经亚型和树突变化，并产生iPS细胞/类器官， 从携带KDM 5C突变的患者样本中区分神经元。核心保持高标准， 通过预定的内部评估和行政管理部门进行的年度用户调查进行质量控制 核心总的来说，NCEI核心代表了遗传学与 认知表型，因此对IDDRC进行的研究产生了广泛而重大的影响 研究人员和他们的受训人员关注的是对IDDs的理解和治疗，这对IDDs的理解和治疗至关重要。