Cell and Molecular Imaging Core (CMI)

细胞和分子成像(CMI)核心的目标是为RFK-IDDRC调查人员提供光学、电子和相关显微镜技术和图像处理方面的全面专家指导、培训和协助，以及获得昂贵的最先进设备。鉴于这一核心在生物学研究中的广泛和基本适用性，预计绝大多数IDDRC调查人员将利用这一核心。这个核心，以前被称为形态核心，在爱因斯坦已经存在了20多年，从两个宽视场光学显微镜和一个电子显微镜发展到一个 多个设施的概要。它对调查人员公认的广泛效用也得到了神经科学部的慷慨财政支持，既可以维持人员，也可以在缺乏NICHD赞助的中心资金的情况下购买额外的设备。这使得Core得以持续增长，并为肯尼迪中心内外的调查人员提供不间断的服务。 核心资源包括专门的宽视场显微镜和电子显微镜，用于国际发展研究中心调查人员的各种标本和目的。样本来自各种动物物种，包括细胞、组织切片或切片、器官类型制剂或整个生物体。IDDRC研究人员的特殊显微镜目标是多样的，包括侧重于细胞组织或细胞结构、细胞内细胞器分布和病理标记物变化等领域的研究。还进行活细胞或组织成像研究，包括用于分析细胞器运输和周转的示踪剂研究。所有研究都得益于有经验的核心人员的专家建议和协助，然后是全面的图像采集和分析。Core的用途进一步扩大，因为它提供了用于基因表达的高分辨率激光辅助解剖和组织和细胞准备的蛋白质组学研究的设备，以及方便地提供裸露DNA构建物的生物技术。其目标不仅是便利高端设备的使用，而且还就所有相关方法向调查人员提供教育和提供高度个性化的咨询和支持，从最初的标本准备到数据的最后包装以供出版或介绍。

The goal of the Cell and Molecular Imaging (CMI) Core is to provide RFK-IDDRC investigators with a comprehensive package of expert guidance, training and assistance in optical, electron and related microscopy techniques and image processing, together with access to costly state-of-the-art equipment. It is expected that the vast majority of IDDRC investigators will make use of this Core given its widespread and fundamental applicability to biological studies. This Core, previously known as the Morphology Core, has existed at Einstein for over 20 years, and has grown from two widefield light microscopes and one electron microscope to a compendium of multiple facilities. Its recognized broad utility to investigators also garnered generous financial support from the Department of Neuroscience, both to sustain personnel as well as to provide for purchase of additional equipment during the absence of NICHD-sponsored center funding. This permitted continued Core growth and uninterrupted service to investigators both inside and outside of the Kennedy Center. Core resources include specialized widefield microscopes and electron microscopes that are utilized for a wide array of specimens and purposes by IDDRC investigators. Samples come from a variety of animal species and include cells, tissue sections or slices, organotypic preparations or whole organisms. Specific microscopy goals for IDDRC investigators are diverse and include studies focused on areas such as cell organization or cytoarchitecture, intracellular organelle distribution, and changes in pathologic markers. Live cell or tissue imaging studies are also carried out, including tracer studies for analysis of organelle transport and turnover. All studies benefit from expert advice and assistance from experienced Core personnel followed by comprehensive image acquisition and analysis. Utility of the Core is further extended by its provision of equipment for high-resolution laser-assisted dissection for gene expression and proteomic studies on tissue and cell preparations, and biolistic technology for facile delivery of naked DNA constructs. The goal is not only to facilitate high-end equipment use, but also to educate and provide highly personalized advice and support to investigators on all relevant methods from initial specimen preparation to final packaging of data for publication or presentation.