Nebraska Center for Nanomedicine- Bioimaging Core

摘要 生物成像是一种必不可少的研究工具。蛋白质和蛋白质的高保真解剖定位能力 纳米递送剂是开发新疗法不可缺少的组成部分。此外，新的 成像模式允许以高度敏感的方式结合结构，以在小范围内以高分辨率工作 动物。因此，我们将利用单一核心结构来容纳三种工具，这些工具将由 与这名科布雷有关的调查人员。小动物磁共振成像(MRI)将可用 使用两台临床前7特斯拉MRI扫描仪，一台Bruker Biospec 7T/21 cm MRI/MRS系统操作Avance III硬件和一套Bruker Pharmascan系统(7T/16厘米)。单独使用高频超声波(高达40兆赫) 并结合光声激光成像将使用的视觉超音速2100和3100仪器 配备了LAZR-X技术。最后，一种新的最先进的活体内显微镜系统包括 奥林巴斯FVMPE-RS双线激光多光子激光共聚焦显微镜。生物成像核心将是 由与科布雷合作多年的三名经验丰富的资深教职员工指导。这个 核心董事将监督每一件仪器的功能和直接技术人员。在……里面 与Core合作，他们将监测支出，并在必要时建议调整用户费用。 重要的是，生物成像核心将为调查人员提供特定用途的基本培训。给定 考虑到这些工具的能力，预计将进行与以下方面有关的各种研究 纳米医学。这包括纳米颗粒的生物分布、细胞跟踪、血红蛋白饱和度、血流、 抗体黏附、心脏和血管功能、血管生成以及活体血管和肿瘤成像。基座 根据目前的赠款支持，我们估计一个持续活跃的核心将在使用中增长。核心将积极 致力于开发新的成像工具和推动调查人员研究的独特方法。一把钥匙 这一核心管理的组成部分正在确保其在第三阶段之后的可持续性。 机构支持与用户费用调整相结合，将提供长期资金。 相关性 使用临床适用的成像方法进行非侵入性评估是转化为 人类使用纳米药物的途径。接触成像和最先进设备方面的专家 将允许培训新的研究人员使用体内定量方法和重要的 在计划这类实验时需要解决的细节。

ABSTRACT Bioimaging is an essential research tool. The ability for high fidelity anatomical localization of proteins and nanodelivery agents is an indispensable component in the development of new therapeutics. Furthermore, new imaging modalities allow for a highly sensitive way of binding structure to function with high resolution in small animals. As such, we will utilize a single Core structure to house three instruments that will be utilized by investigators associated with this COBRE. Small animal magnetic resonance imaging (MRI) will be available using two preclinical 7 Tesla MRI scanners, one Bruker Biospec 7T/21cm MRI/MRS system operating Avance III hardware and one Bruker Pharmascan system (7T/16 cm). High frequency ultrasound (up to 40 MHz) alone and combined with photoacoustic laser imaging will use the Visual Sonics 2100 and 3100 instruments equipped with the LAZR-X technology. Finally, a new state-of-the-art intravital microscopy system includes an Olympus FVMPE-RS multiphoton laser confocal microscope with 2 line lasers. The bioimaging core will be directed by three experienced senior faculty who have been associated with this COBRE for many years. The Core directors will oversee the function of each piece of instrumentation and direct technical staff. In association with Core they will monitor expenditures and recommend adjustments in user fees as necessary. Importantly, the Bioimaging Core will provide essential training to the investigators for specific uses. Given the capabilities of these instruments, it is anticipated that a wide variety of studies will take place related to nanomedicine. This includes nanoparticle biodistribution, cell tracking, hemoglobin saturation, blood flow, antibody adhesion, cardiac and vascular function, angiogenesis and live vascular and tumor imaging. Based on current grant support we estimate a continuously active Core that will grow in use. The Core will be actively engaged in the development of new imaging tools and unique ways of advancing investigators' research. A key component in the administration of this Core is insuring its sustainability beyond Phase III. Outstanding institutional support combined with adjustment of user fees will provide long-term funding. RELEVANCE Non-invasive evaluation using clinically applicable imaging methods is a critical step in the translational pathway towards human use of nanomedicines. Access to experts in imaging and state-of-the-art equipment will allow for training new investigators in the use of quantitative in-vivo methodologies and the important details to be addressed when planning such experiments.