Computational Methods to Characterize Structure and Dynamics of the Nucleosome Core Particle

表征核小体核心颗粒结构和动力学的计算方法

• 批准号: 10442803
• 负责人: Sharon Marie Loverde
• 金额: $ 49.44万
• 依托单位: COLLEGE OF STATEN ISLAND
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10442803
• 关键词: Affect; Amino Acids; Base Sequence; Binding; Biological Assay; Biophysics; Caliber; Characteristics; Charge; Chicago; Chromatin; Chromatin Fiber; Chromatin Structure; Chromosomes; Cities; Collaborations; Complex; Computing Methodologies; DNA; DNA Packaging; DNA Sequence; DNA-Binding Proteins; Development; Disease; Event; Experimental Designs; Faculty; Free Energy; Genes; Genetic Transcription; Goals; Grant; Histones; Investigation; Island; Laboratories; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Mentors; Metaphase; Methodology; Methods; Modification; Molecular; Mutation; NMR Spectroscopy; New York; New York City; Nucleic Acids; Nucleosome Core Particle; Nucleosomes; Oncogenic; Pathway interactions; Polymerase; Positioning Attribute; Post-Translational Modification Site; Post-Translational Protein Processing; Protein Dynamics; Protein Region; Proteins; Reaction; Recording of previous events; Research; Role; Sampling; Structure; Students; Tail; Talents; Techniques; Testing; Time; Universities; Virus; Work; career; chromatin remodeling; college; computerized tools; dimer; epigenetic marker; helicase; histone modification; human disease; innovation; insight; macromolecular assembly; member; models and simulation; molecular dynamics; molecular modeling; oncohistone; programs; rational design; solid state nuclear magnetic resonance; transcription factor; undergraduate student

The nucleosome core particle (NCP) is the basic building block of chromatin, which is a compact, yet dynamic structure that packages DNA. The NCP consists of a positively charged histone octameric core, surrounded by negatively charged nucleosomal DNA which is wrapped ~ 1.7 times around the core. There are many levels of structure in chromatin organization, ranging from the packaging of DNA around the NCP to compact chromatin fibers with diameters ~ 30 nm to denser chromatin fibers in metaphase chromosome with diameters ~ 1.5 µm. DNA binding proteins such as transcription factors and chromatin remodelers need to bind nucleosomal DNA. Thus, in order for transcription to occur, the nucleosomal DNA needs to unwrap fully or partially from the histone core. Modification of the histone core, commonly known as post-translational modification (PTM), can allow for easier access to nucleosomal DNA through modifications in the structure and dynamics of the NCP. Covalent modifications of either histone proteins or DNA often control gene activity and they are the most important epigenetic markers. Furthermore, mutations in chromatin components, such as the NCP, are also found to be commonly involved in diseases such as cancer. The innovative aspect of this proposal is the development of free energy methods in molecular dynamics to characterize complex reaction coordinates in hierarchical protein-nucleic acid assemblies. The PI will develop new computational methods/reaction coordinates to be used in free energy methods, validate atomistic force fields with local collaborators, and develop further methods to predict the impact of single amino acid mutations on nucleosome stability. We expect that the results from these computational investigations can add additional insight into the rational design of experimental investigations into fundamental chromatin structure. The PI’s laboratory will utilize advanced sampling methods in molecular dynamics to characterize the stability of the nucleosome core particle. The role of nucleic acid sequence, PTM, and oncogenic mutations on stability will be elucidated. Force fields for histone tails will be validated through comparison with NMR studies with local collaborators. New methodology to predict the effect of oncogenic mutations on NCP stability will be developed. Results will be tested by collaborators at MSKCC. The computational force fields and methodologies developed during the course of this proposed work could be used to characterize the interactions of nucleic acids and proteins in other macromolecular assemblies, for example, viruses

核小体核心颗粒（NCP）是染色质的基本组成部分，它是一个紧凑的，但 包装DNA的动态结构。NCP由带正电荷的组蛋白八聚体核心组成， 被带负电荷的核小体DNA包围，核小体DNA围绕核心缠绕约1.7倍。有 染色质组织中的许多层次的结构，从围绕NCP的DNA包装到 直径约30 nm的致密染色质纤维至中期染色体中的致密染色质纤维， 直径~ 1.5 µm。DNA结合蛋白，如转录因子和染色质重塑需要结合 核小体DNA因此，为了使转录发生，核小体DNA需要完全解包，或 部分来自组蛋白核心组蛋白核心的修饰，通常称为翻译后修饰 修饰（PTM）可以允许通过结构修饰更容易地接近核小体DNA， NCP的动态。组蛋白或DNA的共价修饰通常控制基因活性， 它们是最重要的表观遗传标记。此外，染色质组分中的突变，如 也发现NCP通常与癌症等疾病有关。创新的一面是 建议发展分子动力学中的自由能方法来表征复杂反应 在分级蛋白质-核酸组装中的坐标。PI将开发新的计算 方法/反应坐标用于自由能方法，验证原子力场与局部 合作者，并开发进一步的方法来预测单个氨基酸突变对核小体的影响 稳定我们希望这些计算研究的结果可以增加对 对染色质基本结构的实验研究的合理设计。PI的实验室将 利用分子动力学中先进的采样方法来表征核小体核心的稳定性 粒子将阐明核酸序列、PTM和致癌突变对稳定性的作用。 组蛋白尾部的力场将通过与当地合作者的NMR研究进行比较来验证。 将开发新的方法来预测致癌突变对NCP稳定性的影响。结果将 由MSKCC的合作者进行测试。计算力场和方法的发展过程中， 这一拟议工作的过程可用于表征核酸和蛋白质的相互作用， 其他大分子组合体，例如病毒