Mechanisms driving cardiac dysfunction in Autosomal Dominant Polycystic Kidney Disease

常染色体显性多囊肾病心脏功能障碍的驱动机制

基本信息

项目摘要

Cardiovascular disease is a major cause of morbidity and mortality in patients with autosomal dominant polycystic kidney disease (ADPKD). Characterized by progressive renal dysfunction, ADPKD imposes very significant healthcare and economic burdens. It has commonly been assumed that progressive renal impairment promotes cardiac disease; however, our preliminary data suggest that cardiac dysfunction originates in cardiomyocytes and manifests prior to renal failure in ADPKD. Recent clinical evidence supports our findings by showing that ADPKD patients exhibit ventricular dysfunction before the onset of renal failure, even in non-hypertensive individuals. Mutations in the gene encoding Polycystin-1 (PC1) occur in 85% of patients and are responsible for the most severe cases. Importantly, PC1 is expressed in cardiomyocytes, yet its role(s) there is(are) poorly understood. We propose that the mutant PC1 – in a cardiomyocyte-autonomous fashion – initiates and drives heart disease in ADPKD, independent of renal failure. Our data show that PC1 cardiomyocyte-specific deletion promotes systolic and diastolic dysfunction in mice. Furthermore, using a mouse model harboring a clinically established ADPKD-causing PC1 mutation (RC allele), we provide evidence of impaired calcium-cycling and contractility at the cardiomyocyte level, which occur before the onset of renal failure. Heterozygous RC/+ young mice manifest alterations in calcium handling/contractility in isolated cardiomyocytes, which correlate with reduced left ventricular global longitudinal strain and diastolic dysfunction. We discovered that PC1 regulates action potential duration via Kv channel current regulation. PC1 ablation shortens action potential duration and impairs both calcium transients and contractility in cardiomyocytes. Additionally, PC1 deletion impairs sarcoplasmic reticulum (SR) calcium loading through reduced SR calcium-ATPase (SERCA) activity. These data have led us to hypothesize that ADPKD-causing PC1 mutations disrupt PC1 actions in cardiomyocytes, impair cardiac function and predispose the heart to hypertension-induced heart failure, independent of renal dysfunction. To test this hypothesis, we propose three aims: 1) determine how PC1 mutations affect action potentials and Kv channel activity and impinge on calcium handling and contractility. 2) elucidate mechanisms whereby PC1 regulates SR calcium loading and SERCA to maintain cardiomyocyte function and test the impact of ADPKD mutations in PC1 on these events. 3) determine in vivo whether alterations in PC1 signaling in cardiomyocytes drive cardiac dysfunction and predispose the heart to hypertension-induced heart failure. Completion of our studies will provide paradigm-shifting information regarding the role of cardiomyocyte-autonomous events driving heart disease in ADPKD, the leading cause of death in these patients.
心血管疾病是常染色体显性多囊肾病(ADPKD)患者发病和死亡的主要原因。以进行性肾功能障碍为特征,ADPKD带来了非常显著的医疗和经济负担。人们通常认为进行性肾损害会促进心脏病;然而,我们的初步数据表明,心功能障碍起源于心肌细胞,并在ADPKD患者肾功能衰竭之前表现出来。最近的临床证据支持我们的发现,表明ADPKD患者在肾衰竭发作前就表现出心室功能障碍,即使在非高血压个体中也是如此。编码多囊蛋白-1 (PC1)的基因突变发生在85%的患者中,是导致最严重病例的原因。重要的是,PC1在心肌细胞中表达,但其在心肌细胞中的作用尚不清楚。我们提出突变体PC1 -以心肌细胞自主的方式-在ADPKD中启动并驱动心脏病,独立于肾功能衰竭。我们的数据显示,PC1心肌细胞特异性缺失促进小鼠收缩和舒张功能障碍。此外,通过使用含有临床建立的adpkd引起的PC1突变(RC等位基因)的小鼠模型,我们提供了心肌细胞水平钙循环和收缩性受损的证据,这些损伤发生在肾衰竭发生之前。杂合子RC/+年轻小鼠在离体心肌细胞中钙处理/收缩能力发生改变,这与左心室整体纵向应变降低和舒张功能障碍有关。我们发现PC1通过Kv通道电流调节来调节动作电位持续时间。PC1消融缩短动作电位持续时间,损害心肌细胞钙瞬态和收缩性。此外,PC1缺失通过降低SR钙- atp酶(SERCA)活性损害了肌浆网(SR)钙负荷。这些数据使我们假设adpkd引起的PC1突变破坏了PC1在心肌细胞中的作用,损害了心功能,并使心脏易发生高血压性心力衰竭,而不依赖于肾功能障碍。为了验证这一假设,我们提出了三个目标:1)确定PC1突变如何影响动作电位和Kv通道活性以及对钙处理和收缩性的影响。2)阐明PC1调节SR钙负荷和SERCA以维持心肌细胞功能的机制,并测试PC1中ADPKD突变对这些事件的影响。3)在体内确定心肌细胞PC1信号的改变是否会导致心功能障碍,并使心脏易发生高血压性心力衰竭。我们研究的完成将提供关于心肌细胞自主事件在ADPKD中驱动心脏病的作用的范式转变信息,ADPKD是这些患者死亡的主要原因。

项目成果

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Francisco Altamirano其他文献

Francisco Altamirano的其他文献

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{{ truncateString('Francisco Altamirano', 18)}}的其他基金

Cardiac Autonomic Activation In Atrial Fibrillation Triggers And Substrate
心房颤动的心脏自主激活触发因素和基质
  • 批准号:
    10636441
  • 财政年份:
    2023
  • 资助金额:
    $ 40.38万
  • 项目类别:
Mechanisms driving cardiac dysfunction in Autosomal Dominant Polycystic Kidney Disease
常染色体显性多囊肾病心脏功能障碍的驱动机制
  • 批准号:
    10618336
  • 财政年份:
    2022
  • 资助金额:
    $ 40.38万
  • 项目类别:

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