A 0.5 mm resolution total-body small-animal PET

分辨率为 0.5 毫米的小动物全身 PET

• 批准号: 10446975
• 负责人: Junwei Du
• 金额: $ 67.73万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10446975
• 关键词: Animals; Anodes; Aorta; Area; Biochemical; Biological Assay; Biological Models; Biological Monitoring; Biological Process; Brain; Brain imaging; Breast Microcalcification; Caliber; Cathodes; Coupled; Crystallization; Development; Disease model; Electronics; Evaluation; Event; Funding; Glues; Goals; Human; Hybrids; Image; Imaging Techniques; Inflammation; Length; Light; Lutetium; Magnetic Resonance Imaging; Measurement; Methods; Mus; Optics; Outcome; Output; Performance; Perfusion; Physiological; Polishes; Positioning Attribute; Positron-Emission Tomography; Protocols documentation; Resolution; Signal Transduction; Silicon; Structure; Study models; System; Technology; Thick; Time; Work; Yttrium; base; cost; data acquisition; design; detector; imaging modality; improved; in vivo; kinetic model; molecular imaging; mouse model; nanomolar; parametric imaging; photomultiplier; pre-clinical; pre-clinical research; preclinical imaging; preclinical study; radiotracer; temporal measurement; tool; ultraviolet

Summary The key parameters of small-animal positron emission tomography (PET) are its spatial resolution and sensitivity that determine the ability to image and quantify radiotracers in a small region of the subject at sub-nanomolar concentrations. However, the applications of small-animal PET have been limited in its application by a combination of spatial resolution and sensitivity, which hampers the use of PET for a range of applications including mouse brain imaging, aortic microcalcification and inflammation imaging, high-resolution total-body dynamic imaging and kinetic modeling studies with using image-derived input function. The goals of this proposal are 1) to develop a 0.5 mm resolution total-body small-animal PET scanner dedicated for high-resolution and fast dynamic applications for imaging mouse disease models, and 2) provide a pre-clinical tool using mouse models to develop, validate, characterize the paradigms and protocols that will feed into human studies, such as total-body and brain studies. The proposed PET scanner will have 128 depth-of-interaction (DOI) detectors. The ring diameter and the axial length are 110 mm and 167 mm, respectively, which is designed to cover the entire body of the mouse and to obtain high resolution and high sensitivity across the entire body. Dual-ended readout detectors based on linearly-graded silicon photomultipliers (LG-SiPMs) coupled to both ends of 40 x 40 lutetium yttrium oxyorthosilicate (LYSO) arrays with a pitch size of 0.5 mm and length of 20 mm will be used to extract DOI information to maintain high and uniform spatial resolution across the entire body of the mouse. LYSO is chosen due to its high light yield (for high spatial resolution and energy resolution), high stopping power (for high sensitivity), and fast decay time (high event rate ability). Dedicated data acquisition electronics for dual-ended readout detectors will be designed for the proposed scanner by upgrading our well-studied electronics system, and a signal multiplexing readout method will be used to reduce the 40 signals of each detector to 9 signals (8 for position information and 1 for timing information) to reduce the cost, complexity, and heat of the readout electronics. The outcome of this proposal will be a total-body small animal PET scanner with a resolution < 0.5 mm and a sensitivity ~24.5 % at the center of the field of view. The resolution will be ~0.5 mm and the sensitivity will be better than 15 % across the volume of the mouse. The spatial/volume resolution is more than 2x/8x better than currently available small-animal PET scanners with similar sensitivity, and the sensitivity is more than 20x better than currently available small-animal PET scanners with similar resolution. The proposed scanner can promote the use of total-body small-animal PET for monitoring biological processes that result in fine structures and expand the range of applications for this powerful, in vivo, non-invasive and translational imaging modality in preclinical applications. The PET scanner developed in this proposal is also MRI-compatible and will support eventual integration inside an MRI scanner for hybrid PET/MRI imaging.

总结 小动物正电子发射断层扫描（PET）的关键参数是其空间分辨率和灵敏度 其确定在受试者的小区域中以亚纳摩尔（sub-nanomolar）的放射性示踪剂成像和定量的能力 浓度的然而，小动物PET的应用在其应用中受到限制， 空间分辨率和灵敏度的组合，这阻碍了PET在一系列应用中的使用 包括小鼠脑成像，主动脉微钙化和炎症成像，高分辨率全身成像， 动态成像和动力学建模研究，使用图像导出的输入函数。 本提案的目标是：1）开发一种0.5 mm分辨率的全身小动物PET扫描仪， 用于成像小鼠疾病模型的高分辨率和快速动态应用，以及2）提供临床前 使用小鼠模型开发，验证，表征将输入人类的范例和协议的工具 研究，如全身和大脑研究。拟议的PET扫描仪将具有128个交互深度 (DOI)探测器环直径和轴向长度分别为110 mm和167 mm， 以覆盖鼠标的整个身体并在整个身体上获得高分辨率和高灵敏度。 基于两端耦合的线性渐变硅光电倍增管（LG-SiPM）的双端读出探测器 40 x 40的氧硅酸镥钇（LYSO）阵列，间距为0.5 mm，长度为20 mm， 用于提取DOI信息，以在整个车身上保持高且均匀的空间分辨率。 老鼠.选择LYSO是因为它的高光产率（用于高空间分辨率和能量分辨率），高停止 功率（高灵敏度）和快速衰减时间（高事件率能力）。 专用的数据采集电子双端读出探测器将被设计为拟议的 扫描仪通过升级我们的研究电子系统，并将使用信号复用读出方法 将每个检测器的40个信号减少到9个信号（8个用于位置信息，1个用于定时信息）， 降低读出电子设备的成本、复杂性和热量。 该提案的成果将是分辨率< 0.5 mm的全身小动物PET扫描仪， 在视场中心的灵敏度约为24.5%。分辨率约为0.5 mm，灵敏度为 在小鼠的体积上优于15%。空间/体积分辨率优于2x/8x 目前可用的小动物PET扫描仪具有相似的灵敏度，并且灵敏度高出20倍以上 比目前可用的小动物PET扫描仪具有类似的分辨率。建议的扫描仪可以促进 使用全身小动物PET监测导致精细结构的生物过程， 扩大了这种强大的、体内的、非侵入性的和平移的成像方式的应用范围， 临床前应用。本提案中开发的PET扫描仪也与MRI兼容， 最终集成在MRI扫描仪内，用于混合PET/MRI成像。