The Neural Circuit Basis of Olfactory Navigation in Adult Drosophila

项目总结 为了有效地寻找食物，大脑必须整合与生俱来的和学习到的关于食物的信息 不同食物气味的价值，并使用这些信息来选择导航机动程序。尽管一个 关于大脑如何计算气味刺激值，如何使用这些信息，我们已经知道了很多 如何引导导航仍然是个谜。在这里，我们建议调查保守派的角色 嗅觉导航中的导航中枢，即扇形体(FB)。FB收到了大量的 在解剖学上位于先天和后天嗅觉处理中心下游的输入。我们有 确定了几条标明这类输入对诱人气味作出反应并推动迎风方向的线 当被激活时。在这里，我们将使用全细胞电生理学和成像来研究 Fb传入神经元中先天和后天气味值信号的表现。通过将这些组合起来 功能数据和详细的连接信息，我们将生成与 感官表现到解剖连通性。接下来，我们将同时使用同步和异步 用于测量FB神经元活动和导航行为之间关系并建立其模型的记录 选择。最后，我们将开发一种新的光遗传羽流测试，这将使我们能够确定 FB神经元在气味羽流导航的不同方面的研究。这些实验和模型将结合在一起 生成关于中央导航电路的组织如何支持关键 目标导向的行为，气味导向的食物搜索。

PROJECT SUMMARY In order to forage effectively for food, the brain must integrate innate and learned information about the value of different food odors and use this information to select navigational motor programs. Although a great deal is known about how the brain computes the value of odor stimuli, how it uses this information to guide navigation remains mysterious. Here we propose to investigate the role of a conserved navigation center, the fan-shaped body (FB), in olfactory navigation. The FB receives a large number of inputs that are anatomically downstream of innate and learned olfactory processing centers. We have identified several lines labeling such inputs that respond to attractive odor and drive upwind orientation when activated. Here we will use whole-cell electrophysiology and imaging to investigate the representation of innate and learned odor value signals in FB input neurons. By combining these functional data with detailed connectomic information, we will generate quantitative models that relate sensory representations to anatomical connectivity. Next we will use both synchronous and asynchronous recordings to measure and model the relationship between FB neuron activity and navigational action selection. Finally, we will develop a new optogenetic plume assay that will allow us to determine the role of FB neurons in different aspects of odor plume navigation. Together these experiments and models will generate testable predictions about how the organization of central navigation circuits supports a critical goal-directed behavior, odor-guided food search.