Neural Circuits Underlying Multisensory Control of Orientation in Drosophila

果蝇方向多感官控制的神经回路

• 批准号: 10346734
• 负责人: Katherine Nagel
• 金额: $ 9.65万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10346734
• 关键词: Animal Model; Behavior; Brain; Brain region; Cells; Complex; Cues; Data; Drosophila genus; Ear; Electrophysiology (science); Environmental Wind; Eye; Flying body movement; Functional Imaging; Literature; Modality; Modeling; Movement; Neurons; Nose; Odors; Pathway interactions; Role; Sensory; Stimulus; Stream; Sum; Testing; Walking; fly; multisensory; neural circuit; novel; optogenetics; sensory stimulus; visual stimulus

Project Summary/Abstract: How the brain combines sensory stimuli from different modalities to guide behavior is currently not understood at a single-cell level. Here we propose to use the model organism Drosophila to investigate the neural circuit basis of multi-sensory integration in the context of two specific orientation behaviors developed in our lab. In one paradigm, odor switches the orientation of walking flies to a wind (mechanosensory) cue: flies orient downwind in the absence of odor and upwind in the presence of an attractive odor. In the second paradigm, an aversive wind stimulus and attractive visual stimulus sum to guide orientation of a tethered flying fly. We have discovered a novel mechanosensory pathway that computes wind direction from movements of the fly's antennae, and transmits this information to the central complex, a region of the brain implicated in navigation. Combined with information from the literature and preliminary data from our own lab, this suggests a model for how different sensory streams might be aligned and integrated within the central complex. We propose to use optogenetic activation, chemogenetic silencing, whole-cell electrophysiology, and functional imaging to test the role of candidate central complex neurons in multi- sensory control of orientation. We will additionally test the alternative hypothesis that multi-sensory integration in descending neurons is required for the two behaviors. This study will provide a comprehensive cellular-level account of how sensory streams are combined to control the orientation of a model organism.

项目摘要/摘要： 大脑如何结合来自不同形式的感觉刺激来指导行为目前还不是很清楚 在单细胞水平上被理解。在这里，我们建议使用模式生物果蝇来研究 两种特定定向行为背景下多感觉整合的神经回路基础 在我们的实验室里。在一种模式中，气味将行走的苍蝇的方向切换到风(机械感觉)提示： 苍蝇在没有气味的情况下顺风而行，在有诱人气味的情况下迎风而行。在第二个 范型，一个令人厌恶的风刺激和吸引人的视觉刺激总和来引导系绳的方向 会飞的苍蝇。我们已经发现了一种新的机械传感路径，它可以从 苍蝇触角的运动，并将这些信息传输到中央复合体，大脑的一个区域 与航海有牵连。结合文献中的信息和我们自己的初步数据 实验室，这提出了一个模型，不同的感觉流可能如何对齐和整合在 中央综合体。我们建议使用光遗传激活、化学发生沉默、全细胞 电生理学和功能成像来测试候选中枢复合体神经元在多发性脑损伤中的作用 对方位的感官控制。我们还将测试另一种假设，即多感官 这两种行为都需要下行神经元的整合。这项研究将提供一个全面的 对感官流如何结合以控制模型生物体方向的细胞级描述。