Neural Circuits Underlying Multisensory Control of Orientation in Drosophila

果蝇方向多感官控制的神经回路

• 批准号: 10647673
• 负责人: Katherine Nagel
• 金额: $ 38.17万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10647673
• 关键词: Adult; Area; Behavior; Behavioral; Behavioral Paradigm; Brain; Brain region; Cell model; Cells; Complex; Coupled; Cues; Data; Detection; Disease; Drosophila genus; Ear; Electrophysiology (science); Environmental Wind; Exhibits; Experimental Models; Eye; Flying body movement; Functional Imaging; Genetic; Genetic Screening; Goals; Grant; Human; Literature; Location; Logic; Memory; Modality; Modeling; Motion; Motor; Movement; Neurons; Nose; Odors; Pathway interactions; Population; Role; Sensory; Shapes; Signal Transduction; Stimulus; Stream; Structure; Sum; Testing; Vertebrates; Visual; Walking; behavioral outcome; flexibility; fly; insight; model organism; motor control; multimodality; multisensory; neural circuit; novel; optogenetics; response; sensory integration; sensory stimulus; transmission process; visual processing; visual stimulus

Project Summary! How the brain combines sensory stimuli from different modalities to guide behavior is currently not understood at a single-cell level. Here we propose to use the model organism Drosophila to investigate the neural circuit basis of multi-sensory integration in the context of two specific orientation behaviors developed in our lab. In one paradigm, odor switches the orientation of walking flies to a wind (mechanosensory) cue: flies orient downwind in the absence of odor and upwind in the presence of an attractive odor. In the second paradigm, an aversive wind stimulus and attractive visual stimulus sum to guide orientation of a tethered flying fly. We have discovered a novel mechanosensory pathway that computes wind direction from movements of the fly's antennae, and transmits this information to the central complex, a region of the brain implicated in navigation. Combined with information from the literature and preliminary data from our own lab, this suggests a model for how different sensory streams might be aligned and integrated within the central complex. We propose to use optogenetic activation, chemogenetic silencing, whole-cell electrophysiology, and functional imaging to test the role of candidate central complex neurons in multi-sensory control of orientation. We will additionally test the alternative hypothesis that multi-sensory integration in descending neurons is required for the two behaviors. This study will provide a comprehensive cellular-level account of how sensory streams are combined to control the orientation of a model organism.!

项目总结！ 大脑如何结合来自不同形式的感官刺激来指导行为， 在单细胞水平上无法理解。在这里，我们建议使用模式生物果蝇 在两个特定的背景下研究多感觉整合的神经回路基础， 在我们实验室发展的定向行为。在一个范例中，气味改变了 行走的猴子对风（机械感觉）线索：猴子在没有气味的情况下顺风定向， 在有吸引力的气味的情况下逆风飞行。在第二种范式中， 刺激物和吸引人视觉刺激物加在一起引导拴系的锚定器的定向。我们有 发现了一种新的机械感觉通路，可以通过运动来计算风向 的触角，并将此信息传输到中央复合体，一个区域的 大脑参与导航。结合文献资料和初步的 从我们自己实验室的数据，这表明了一个模型，如何不同的感觉流可能是 与中央综合体协调一致。我们建议使用光遗传学激活， 化学遗传学沉默，全细胞电生理学和功能成像，以测试 候选的中央复合神经元在多感觉控制方向。我们还将 测试替代假设，即下行神经元中的多感觉整合是必需的 对于这两种行为。这项研究将提供一个全面的细胞水平的帐户如何 感觉流被结合起来控制模式生物的方向。