Core D: Cardiometabolic Phenotyping Core

核心 D：心脏代谢表型核心

• 批准号: 10447068
• 负责人: Jonathan Mark Brown
• 金额: $ 25.76万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10447068
• 关键词: Adipose tissue; Animal Model; Animal Sources; Animals; Area; Atherosclerosis; Bile Acids; Bioinformatics; Blood Platelets; Cardiometabolic Disease; Cells; Clinic; Clinical; Collaborations; Controlled Environment; Coupled; Development; Diet; Disease; Energy Metabolism; Engineering; Ensure; Formulation; Genotype; Germ; Germ-Free; Gnotobiotic; Goals; Human; Individual; Insulin; Investigation; Lesion; Lipids; Lipoproteins; Lyase; Mass Spectrum Analysis; Metabolic; Metabolism; Metagenomics; Methodology; Methods; Modeling; Mus; Obesity; Pathogenesis; Pathway interactions; Performance; Phenotype; Protocols documentation; Reagent; Research Personnel; Research Project Grants; Role; Sampling; Services; Standardization; Sterilization; Study models; Sulfate; System; Technical Expertise; Thrombosis; Tissues; Tracer; Training Support; Transplantation; Universities; Work; bile acid metabolism; blood glucose regulation; cardiometabolism; chemical synthesis; data sharing; design; disease phenotype; gut microbes; gut microbiota; in vivo; inhibitor; insulin sensitivity; investigator training; metabolomics; microbial; microbial community; microbiome analysis; mouse model; novel strategies; pre-clinical; pressure; programs; seal; small molecule; stable isotope; trait; transcriptome sequencing; trimethylamine

Cardiometabolic Phenotyping Core (Core D). ABSTRACT: The overall goal of the Cardiometabolic Disease Phenotyping Core (Core D) is to provide centralized expertise for performance of state-of-the-art animal model studies relevant to Cardiometabolic Diseases in support of Projects within this Program. This core is necessary to establish consistency in mouse phenotyping studies performed across all three Projects, and will ensure seamless data sharing and comparison of how each of the different gut microbial metabolite pathways under investigation impact cardiometabolic disease in mice. All projects in this PPG propose to examine the impact of select gut microbe-derived metabolites on host cardiometbolic disease phenotypes including atherosclerosis, thrombosis, adiposity, insulin/glucose homeostasis, and altered lipid/bile acid metabolism. Each of these phenotypes requires independent rigorous methods to be carried out by well-trained investigators in each area. The Cardiometabolic Disease Phenotyping Core (Core D) will provide technical expertise in a centralized mouse phenotyping service that will collaboratively interface with all other research projects and cores to examine the effect of altering gut microbial metabolites on cardiometabolic disease phenotypes.

心脏代谢表型核心（核心 D）。抽象的： 心脏代谢疾病表型核心（核心 D）的总体目标是提供集中的专业知识 进行与心脏代谢疾病相关的最先进的动物模型研究，以支持 本计划内的项目。该核心对于建立小鼠表型研究的一致性是必要的 在所有三个项目中执行，并将确保无缝数据共享和每个项目的比较 正在研究的不同肠道微生物代谢途径对小鼠心脏代谢疾病的影响。全部 该 PPG 中的项目建议研究特定肠道微生物衍生代谢物对宿主的影响 心脏代谢疾病表型，包括动脉粥样硬化、血栓形成、肥胖、胰岛素/血糖 体内平衡和改变脂质/胆汁酸代谢。这些表型中的每一个都需要独立严格的 由每个领域训练有素的调查人员执行的方法。心脏代谢疾病 表型分析核心（核心 D）将提供集中式小鼠表型分析服务的技术专业知识，该服务将 与所有其他研究项目和核心协作接口，以检查改变肠道的效果 微生物代谢物对心脏代谢疾病表型的影响。