Whole Genome Sequencing of foodborne pathogens

FDA的食品安全和应用营养中心(CFSAN)创建了第一个 州和联邦实验室综合网络(GenomeTrakr)使用全基因组 用于跟踪食源性病原体的测序以提高暴发反应和有效性 对预防性控制的监测。GenomeTrakr网络已经创建了一个公开的 现有的全球数据库包含数千种食源性疾病的基因构成 造成了有机体。本项目意在发展更强大的国际化快速 进一步加强GenomeTrakr网络的病原体溯源监测系统 以及不断增长的数据库。准确和及时地进行分类和后续的分类 与食源性暴发事件相关的细菌分离是成功的关键 调查并最终追溯到特定的食物或环境来源。基因组学 GenomeTrakr网络提供的信息将调查人员引向特定的食品 可能与暴发有关，并提供对受污染食品来源的洞察。 该项目将促进公共卫生和国家农业实验室顺序致病 将食源性细菌添加到在线数据库中。将需要当地的实验室 对至少250个食物、环境或动物分离株进行测序，以添加到 数据库，以识别未来的疫情。当地的实验室也将被要求参加 GenomeTrakr在标准过程中需要测序和分析的一致性，而 还参加会议、会议和能力测试，以确保标准化 在实验室之间。

The FDA’s Center for Food Safety and Applied Nutrition (CFSAN) has created the first integrated network of state and federal laboratories (GenomeTrakr) to use whole genome sequencing to track foodborne pathogens to improve outbreak response and effective monitoring of preventative controls. The GenomeTrakr network has created a publically available, global database containing the genetic makeup of thousands of foodborne disease causing organisms. This project is intended to develop a stronger international rapid surveillance system for pathogen trace-back by further enhancing the GenomeTrakr network and the growing database. The accurate and timely subtyping and subsequent clustering of isolates of a bacterium associated with a foodborne outbreak event is essential for successful investigation and eventual trackback to a specific food or environmental source. The genomic information provided by the GenomeTrakr network points investigators to specific food products potentially related to an outbreak, and provides insight into the origin of the contaminated food. This project will promote public health and state agricultural laboratories sequence pathogenic foodborne bacterium to be added to the on-line database. The local laboratories will be required to sequence a minimum of 250 food, environmental or animal isolates to be added to the database to identify future outbreaks. The local laboratories will also be required to participate in GenomeTrakr in a standard process requiring consistency of the sequencing and analysis while also participating in meetings, conferences and proficiency tests to ensure standardization between laboratories.