Core A: Animal Models Core

核心 A：动物模型核心

• 批准号: 10447739
• 负责人: Ryan B Sartor
• 金额: $ 24.97万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-19 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10447739
• 关键词: Animal Model; Animals; Bacteria; Breeding; Cell Communication; Cell Lineage; Cells; Chronic; Complex; Country; Crossbreeding; Derivation procedure; Development; Ecology; Environment; Epithelial; Epithelial Cells; Faculty; Funding; Genes; Genetic; Genetic Transcription; Germ-Free; Gnotobiotic; Goals; Homeostasis; Housing; Human; Human Resources; Immune; Immune response; Immunocompromised Host; Individual; Intestinal Mucosa; Intestines; Laboratories; Leadership; Maintenance; Mammals; Measures; Mediating; Microbe; Modeling; Mouse Strains; Mucous Membrane; Mus; Mutant Strains Mice; Phenotype; Physiological; Proteins; Relapse; Reporter; Research Personnel; Resolution; Resources; Rodent; Role; Services; Stretching; Tissues; Transgenic Mice; Transgenic Organisms; Universities; Variant; Washington; Work; Zebrafish; animal facility; animal resource; cell type; cost; cost effective; experience; experimental study; fecal transplantation; germ free condition; gut inflammation; gut microbiota; improved; in vivo imaging; innovation; intestinal barrier; intestinal homeostasis; microbial; microbial host; microbiota; mouse model; mutant; novel; prevent; programs; response; success; wasting

The Animal Models Core centralizes breeding, development and maintenance of extremely useful, but complex mouse and zebrafish models by providing a stable environment for each centrally housed line to increase efficiency, decrease costs and prevent phenotype variation. This Core leverages exceptional specific pathogen free (SPF) and gnotobiotic mice and zebrafish facilities and expert personnel to provide high-quality resources and services to all 3 current and proposed Projects. Core A personnel work closely with Project investigators to provide SPF and gnotobiotic mouse and zebrafish models that are essential to accomplish goals of each of the current projects. This core provides prioritized creation of novel SPF mouse strains and derivation of germ-free (GF) lines that will support the success of Projects 1, 2, 3 and 4. In addition, this Core has created highly innovative zebrafish models to facilitate activities in Projects 3 and 4. By providing technical experience and central housing to create and maintain GF wild type, mutant and double mutant mouse strains, Core A allows investigators to efficiently perform innovative, mechanistic experiments far beyond the resources of traditional R01-supported investigators and extends the capabilities of our national gnotobiotic resource. These GF mice and zebrafish allows Project investigators to manipulate the complex gut microbial ecology to study the mechanistic role of luminal microbes on immune and epithelial responses in gnotobiotic animals colonized with defined microbiota. The Specific Aims of the Animal Models Core are to provide P01 Projects with: 1.Centralized breeding of specific pathogen free and germ-free mice and zebrafish to eliminate redundancy in breeding units, diminish expenses, decrease environmental variation by maintaining central housing facilities and minimize waste by promoting shared utilization of animals by multiple investigators; 2.Create new mouse and zebrafish models including importing existing mutant strains for central breeding, creating new strains, including cell-specific mutants by crossbreeding of existing mutant, transgenic and fluorescent protein reporter lines, and generating new transgenic and mutant lines; 3.Germ-free derivation of existing and new mouse strains that can be used to evaluate the impact of complex resident microbiota individual microbial species or microbial consortia on intestinal inflammation and mucosal homeostasis. This Core supports Projects 1, 2, 3 and 4 in the renewal application.

动物模型核心集中培育、开发和维护极其有用的，但 通过为每个集中安置的线路提供稳定的环境来提供复杂的老鼠和斑马鱼模型 提高效率，降低成本，防止表型变异。此核心利用特殊的特定功能 无病原体(SPF)和灵知菌小鼠和斑马鱼设施和专家人员提供高品质 为所有3个当前和拟议的项目提供资源和服务。 核心A人员与项目调查人员密切合作，提供SPF和灵芝小鼠和 斑马鱼模型对于实现当前每个项目的目标至关重要。这个核心提供了 优先创造新的SPF小鼠品系，并衍生出支持 项目1、2、3和4的成功。此外，该核心创建了高度创新的斑马鱼模型，以 促进项目3和4的活动。通过提供技术经验和中央住房来创造和 保持GF野生型、突变型和双突变小鼠品系，核心A使研究人员能够高效地 执行创新的机械实验，远远超出传统R01支持的资源 调查并扩展我们国家灵芝资源的能力。这些GF老鼠和斑马鱼 允许项目研究人员操纵复杂的肠道微生物生态来研究 管腔微生物对定植有特定微生物区系的灵知生物群动物免疫和上皮反应的影响。 动物模型核心的具体目标是为P01项目提供： 1.集中饲养无特定病原体和无菌小鼠和斑马鱼，以消除 繁育单位的冗余，减少开支，通过维护中心减少环境变化 安置设施，并通过促进多名调查员共同使用动物来最大限度减少浪费； 2.创造新的小鼠和斑马鱼模型，包括引进现有的突变品系进行集中育种， 创造新的菌株，包括通过杂交现有的突变体、转基因和 荧光蛋白报告系，并产生新的转基因和突变系； 3.现有和新的小鼠品系的无菌衍生，可用于评估 复杂的驻留微生物区系单个微生物物种或微生物群体对肠道炎症和 粘膜动态平衡。 此核心支持续签申请中的项目1、2、3和4。