权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Core A: Animal Models Core

核心 A：动物模型核心

基本信息

批准号：
10447739
负责人：
Ryan B Sartor
金额：
$ 24.97万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-19 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10447739
关键词：
Animal Model Animals Bacteria Breeding Cell Communication Cell Lineage Cells Chronic Complex Country Crossbreeding Derivation procedure Development Ecology Environment Epithelial Epithelial Cells Faculty Funding Genes Genetic Genetic Transcription Germ-Free Gnotobiotic Goals Homeostasis Housing Human Human Resources Immune Immune response Immunocompromised Host Individual Intestinal Mucosa Intestines Laboratories Leadership Maintenance Mammals Measures Mediating Microbe Modeling Mouse Strains Mucous Membrane Mus Mutant Strains Mice Phenotype Physiological Proteins Relapse Reporter Research Personnel Resolution Resources Rodent Role Services Stretching Tissues Transgenic Mice Transgenic Organisms Universities Variant Washington Work Zebrafish animal facility animal resource cell type cost cost effective experience experimental study fecal transplantation germ free condition gut inflammation gut microbiota improved in vivo imaging innovation intestinal barrier intestinal homeostasis microbial microbial host microbiota mouse model mutant novel prevent programs response success wasting

项目摘要

The Animal Models Core centralizes breeding, development and maintenance of extremely useful, but complex mouse and zebrafish models by providing a stable environment for each centrally housed line to increase efficiency, decrease costs and prevent phenotype variation. This Core leverages exceptional specific pathogen free (SPF) and gnotobiotic mice and zebrafish facilities and expert personnel to provide high-quality resources and services to all 3 current and proposed Projects. Core A personnel work closely with Project investigators to provide SPF and gnotobiotic mouse and zebrafish models that are essential to accomplish goals of each of the current projects. This core provides prioritized creation of novel SPF mouse strains and derivation of germ-free (GF) lines that will support the success of Projects 1, 2, 3 and 4. In addition, this Core has created highly innovative zebrafish models to facilitate activities in Projects 3 and 4. By providing technical experience and central housing to create and maintain GF wild type, mutant and double mutant mouse strains, Core A allows investigators to efficiently perform innovative, mechanistic experiments far beyond the resources of traditional R01-supported investigators and extends the capabilities of our national gnotobiotic resource. These GF mice and zebrafish allows Project investigators to manipulate the complex gut microbial ecology to study the mechanistic role of luminal microbes on immune and epithelial responses in gnotobiotic animals colonized with defined microbiota. The Specific Aims of the Animal Models Core are to provide P01 Projects with: 1.Centralized breeding of specific pathogen free and germ-free mice and zebrafish to eliminate redundancy in breeding units, diminish expenses, decrease environmental variation by maintaining central housing facilities and minimize waste by promoting shared utilization of animals by multiple investigators; 2.Create new mouse and zebrafish models including importing existing mutant strains for central breeding, creating new strains, including cell-specific mutants by crossbreeding of existing mutant, transgenic and fluorescent protein reporter lines, and generating new transgenic and mutant lines; 3.Germ-free derivation of existing and new mouse strains that can be used to evaluate the impact of complex resident microbiota individual microbial species or microbial consortia on intestinal inflammation and mucosal homeostasis. This Core supports Projects 1, 2, 3 and 4 in the renewal application.

动物模型核心集中育种，开发和维护极其有用，但