Automatic and accurate identification of aerobic bacteria, anaerobic bacteria, yeasts, and fungi in clinical samples derived from animals and from feed for pets

项目摘要 该项目旨在建立在康涅狄格州兽医医学诊断实验室 （CVMDL）自动、快速、准确地识别 需氧菌、厌氧菌、酵母菌和真菌 动物和宠物的商业饲料中。识别是一个步骤， 评估细菌和真菌分离株的抗菌素耐药性。实验室 需要取代现在被认为过时的自动识别系统用于身份证 好氧细菌（Ommnilog系统）与一个新的系统，提供更广泛的 能力和更高的准确性。 该项目的目标与美国FDA兽医实验室调查一致， 响应网络（Vet-LIRN）努力跟踪抗生素耐药性， 从患病动物和饲料中分离的细菌和真菌。 为了实现这个项目的目标，我们正在申请资金，以获得一个自动化的 系统如GENIIIMomniLogIDPlus系统（Biolog Inc.，海沃德，CA，USA）和 伴随数据库包括YT（酵母）、FF（真菌）、AN（厌氧菌）和 GEN III用于革兰氏阴性和阳性细菌。这种Biolog高级表型 技术提供了关于菌株特性的有价值的信息， 物种级鉴定在该系统中，专有的碳源利用 技术通过产生一种 来自所进行的离散测试反应的特征模式或“代谢指纹” 在96孔微孔板中。 购买GEN III OmniLog ID Plus系统将提高 在CVMDL微生物学实验室充分参与Vet-LIRN目标。的 实验室最近获得了一个先进的自动化系统，以识别细菌和 阅读抗生素敏感性平板（BIOMIC V3，Giles Scientific）。两者的使用 系统将增强CVMDL的能力。

Project Summary This project aims to build at the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) the capacity necessary to automatically, rapidly, and accurately identify aerobic bacteria, anaerobic bacteria, yeasts, and fungi in clinical samples derived from animals and in commercial feed for pets. Identification is a step needed before assessing the antimicrobial resistance of bacterial and fungal isolates. The laboratory needs to replace a now considered obsolete automatic identification system used for ID aerobic bacteria (Ommnilog system) with a new system that provides broader capabilities and improved accuracy. The project's goal aligns with the US FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) efforts towards tracking antimicrobial resistance in bacteria and fungi isolated from sick animals and feed. To meet the goal of this project, we are requesting funds to acquire an automated system like GEN III OmniLog ID Plus System (Biolog Inc., Hayward, CA, USA) and companion databases including YT (yeast), FF (fungi), AN (anaerobic bacteria) and GEN III for Gram-negative and positive bacteria. This Biolog advanced phenotypic technology provides valuable information on the properties of strains, in addition to a species-level identification. In the system, a proprietary carbon source utilization technology identifies environmental and pathogenic microorganisms by producing a characteristic pattern or "metabolic fingerprint" from discrete test reactions performed within a 96 well microplate. The purchase of a GEN III OmniLog ID Plus System system will enhance the capacity at the CVMDL Microbiology Laboratory to be fully engaged with Vet-LIRN goals. The laboratory recently acquired an advanced automated system to identify bacteria and for reading antibiotic susceptibility plates (BIOMIC V3, Giles Scientific). The use of both systems will potentiate CVMDL’s capabilities.