Neurobiological significance of Aqp4 stop codon readthrough

Aqp4 终止密码子通读的神经生物学意义

• 批准号: 10451765
• 负责人: Darshan Sapkota
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10451765
• 关键词: 3' Untranslated Regions; APP-PS1; Abeta clearance; Alzheimer' s Disease; Alzheimer' s disease brain; Amyloid beta-Protein; Animal Model; Antibodies; Astrocytes; Basic Science; Behavioral; Biological; Biology; Blood - brain barrier anatomy; Brain; C-terminal; CRISPR interference; Clinical; Clinical Treatment; Data; Defect; Disease; Disease Outcome; Enzyme-Linked Immunosorbent Assay; Ependymal Cell; Epitopes; Etiology; Excision; Exhibits; Fluorescence; Functional disorder; Future; Gene Expression; Gene Expression Regulation; Genetic; Goals; Half-Life; Hippocampus (Brain); Impaired cognition; Impairment; Intercellular Fluid; Knockout Mice; Learning; Link; Long-Term Potentiation; Luciferases; Measures; Membrane; Memory; Mentors; Messenger RNA; Molecular; Morphology; Mus; Neurobiology; Neurons; Outcome; Pathogenesis; Pathway interactions; Phase; Play; Process; Protein Isoforms; Proteins; Regulation; Reporter; Research Personnel; Ribosomes; Role; Sleep; Synapses; Synaptic plasticity; Techniques; Technology; Terminator Codon; Testing; Therapeutic; Time; Transgenic Mice; Translating; Viral; Viral Vector; abeta accumulation; abeta oligomer; aquaporin 4; cell type; chemical genetics; deep sequencing; disorder risk; drug use screening; experimental study; genetic manipulation; improved; improved outcome; in vivo; insight; mouse genetics; mouse model; neurofibrillary tangle formation; neuron loss; new therapeutic target; novel; novel therapeutics; overexpression; preclinical study; prevent; screening; skills; tau Proteins; viral rescue; water channel; β-amyloid burden

Project Summary/Abstract Soluble amyloid beta (Aβ) oligomers trigger tau tangle formation, neuronal cell loss, synaptic dysfunction and cognitive decline seen in Alzheimer's disease (AD). The water channel Aquaporin 4 (Aqp4) is a key component of the Aβ removal machinery in the brain, as evidenced by ~ 55% reduction in Aβ removal in Aqp4-/- mice. Specifically, astrocyte endfeet-concentrated Aqp4 is shown to be both required for removing Aβ during sleep and perturbed in AD, suggesting that restoring Aqp4 to endfeet can improve the outcome of AD. I find that this endfeet-localized Aqp4 is a stop codon readthrough version of Aqp4. I performed ribosome footprinting (RF), deep-sequencing of ribosome-protected mRNA fragments, in the mouse brain and detected reads mapping to the 3' untranslated region of Aqp4, suggesting that ribosomes read past the stop codon and make a C- terminally extended version of Aqp4 (Aqp4X hereafter). Using an antibody against the readthrough epitope, I show that Aqp4X is exclusive to the perivascular endfeet, whereas the normal un-extended Aqp4 is confined elsewhere along the astrocyte membrane. Therefore, the objective of this project is to determine if Aqp4 readthrough enhances Aβ clearance and thus improve AD outcome. I propose 3 aims to meet this objective. In aim 1, I will determine if Aqp4X has altered efficacy in eliminating Aβ compared to Aqp4. I will express either Aqp4X or Aqp4 using viral transduction in the hippocampi of APP/PS1+/- transgenic mice, and use a novel micro-immunoelectrode technology to measure the rate of Aβ removal from the interstitial fluid in live mice. Next, on the Aqp4-/- nice that I have acquired and Aqp4No_X mice that I have generated, I will use ELISA to measure their total brain Aβ levels, with or without viral rescue. I will also examine Aqp4No_X mice for memory and other behavioral deficits. In aim 2, I will identify the chemical and genetic regulators of Aqp4 readthrough using drug screening and CRISPRi screening, respectively. Finally, in aim 3, as an independent investigator, I will determine the AD-related pathophysiological consequences arising from the loss of endfeet Aqp4. To this end, I will examine Aqp4No_X mice for possible structural and functional defects in the BBB and neuronal-activity dependent gene regulation in the hippocampus. I will also cross these mice with APP/PS1 mice and test if Aβ burden and behavioral deficits escalate when an AD mouse loses endfeet Aqp4. Thus, aim 1 will test the necessity and sufficiency of the two Aqp4 versions in Aβ clearance, aim 2 will allow future studies on potential therapeutics and biological regulators, and aim 3 will further elucidate the role Aqp4X plays in AD.

项目总结/摘要 可溶性淀粉样蛋白β（Aβ）寡聚体触发tau缠结形成、神经元细胞损失、突触功能障碍和神经元凋亡。 阿尔茨海默病（AD）中出现的认知能力下降。水通道水通道蛋白4（Aqp 4）是一个关键组成部分， Aqp 4-/-小鼠中Aβ清除减少约55%证明了这一点。 特别是，星形胶质细胞足端浓缩的Aqp 4被证明是在睡眠期间清除Aβ所必需的。 并且在AD中受到干扰，这表明将Aqp 4恢复到末端足可以改善AD的结果。我发现这 末端定位的Aqp 4是Aqp 4的终止密码子通读形式。我进行了核糖体足迹法（RF）， 核糖体保护的mRNA片段的深度测序，在小鼠大脑中，检测到的读数映射到 Aqp 4的3'非翻译区，这表明核糖体阅读通过终止密码子，并使C- Aqp 4的末端延伸形式（下文称为Aqp 4X）。使用针对通读表位的抗体， 表明Aqp 4X仅限于血管周围终足，而正常的未延伸的Aqp 4被限制在血管周围终足， 沿着星形胶质细胞膜。因此，本项目的目标是确定Aqp 4是否 通读增强Aβ清除，从而改善AD结局。 我提出3个目标来实现这一目标。在目标1中，我将确定Aqp 4X是否改变了消除 Aβ与Aqp 4相比。我将使用病毒转导在大肠杆菌中表达Aqp 4X或Aqp 4。 APP/PS1+/-转基因小鼠，并采用新型微量免疫电极技术测定Aβ 从活小鼠的间质液中去除。接下来，在我获得的Aqp 4-/- nice和Aqp4No_X上， 我将使用ELISA来测量它们的总脑Aβ水平，无论是否有病毒感染， 救援我还将检查Aqp4No_X小鼠的记忆和其他行为缺陷。在目标2中，我将确定 使用药物筛选和CRISPRi筛选的Aqp 4通读的化学和遗传调节剂， 分别最后，在目标3中，作为一名独立调查员，我将确定与AD相关的 终足缺失引起的病理生理学后果Aqp 4。为此，我将研究Aqp4No_X 小鼠的BBB和神经元活性依赖性基因调控中可能的结构和功能缺陷 在海马区。我还将这些小鼠与APP/PS1小鼠杂交，并测试Aβ负荷和行为 当AD小鼠失去末端足Aqp 4时，缺陷升级。因此，目标1将检验以下方面的必要性和充分性： Aqp 4在Aβ清除中的两个版本，aim 2将允许未来对潜在的治疗和生物学的研究。 调节因子，目的3将进一步阐明Aqp 4X在AD中发挥的作用。