Central Biochemistry Laboratory of the CKID Consortium

中心生物化学实验室(CBL)为未来的研究对象和研究人员提供服务 儿童慢性肾脏病的研究CBL为参与的临床站点提供 采集血液和尿液进行分析所需的方案、试剂盒、试剂、用品和运输 URMC和其他实验室。血液分析包括电解质、尿素氮、肌酐、葡萄糖、钙、 磷、白蛋白、尿酸、脂质筛查、hsCRP、完整甲状旁腺素和胱抑素C。尿液分析包括 肌酸和蛋白质。CBL已经确定了运输这些分析物的最佳条件，并且 为每次访问提供环境托运人，为冷冻标本提供季度干冰托运人。CBL还 提供收集和运输血液、血浆、血清、尿液、头发和指甲的工具包和说明 样本送到美国国立卫生研究院的储存库。来自每次CKiD访问的生化数据被审查并输入到Nephron， CKiD数据库。CBL在CKiD年度会议上提供培训，并随时可以提供 在CKiD访问期间提供信息、指导和支持。CBL提供了表演方面的专业知识 生化分析和对结果的临床解释。CBL定期与数据合作 协调中心提供实验室医学方面的专业知识，优化GFR研究和 生成GFR估算方程，维护程序手册，确保所有项目的质量控制 通过改变仪器设备，提供生化分析的连续性。CBL 参加所有指导委员会会议和电话会议，并与调查人员在 摘要、演示文稿和手稿的生成。CBL中心的拟议目标是继续 为一般肾脏健康提供准确、精确和有效的生化测量方法 并作为科学伙伴为推进CKiD联盟的研究议程做出贡献。CBL将会 继续提供采集、处理和运输样品的实验室试剂盒，准确接收、 对这些样品进行日常处理和分析，并及时将数据输入Nephron。这个 CBL已经与UMN ARDL建立了一项新的分包合同，将确保准确测量碘海醇 在本项目期间的浓度(并因此GFR)。因此，CBL将继续发展和 在队列3受试者和其他参与者中使用常规生物标志物优化GFR估计方程 第1期慢性肾脏病患者，以及接受肾移植的患者。CBL已经完成了概念上的工作 研究表明，高尿酸血症与肾脏进展更快有关，而微量白蛋白尿 尿蛋白并不能比尿蛋白更好地预测肾脏的进展。CBL接下来将检查贝塔微量蛋白 以确定其在预测肾小球滤过率方面是否有用。长期目标是更好地描述CKiD人群的特征 在生物化学方面，保持数据的完整性，以一种 维持CKiD受试者的招聘和保留，并制作相关出版物。

The Central Biochemistry Laboratory (CBL) serves the subjects and investigators of the continuing Prospective Study of Chronic Kidney Disease in Children (CKiD). The CBL provides participating clinical sites with protocols, kits, reagents, supplies, and transportation required for collecting blood and urine for analysis at URMC and other laboratories. Blood analytes include electrolytes, BUN, creatinine, glucose, calcium, phosphorus, albumin, uric acid, lipid screen, hsCRP, intact PTH, and cystatin C. Urine analytes include creatinine and protein. The CBL has determined optimal conditions for transporting these analytes, and provides ambient shippers for each visit and quarterly dry ice shippers for frozen specimens. The CBL also provides the kits and instructions for collection and shipment of blood, plasma, sera, urine, hair, and nail samples to NIH repositories. Biochemical data from each CKiD visit are reviewed and entered into Nephron, the CKiD data base. The CBL provides training at annual CKID meetings and is readily available to provide information, instruction, and support during CKiD visits. The CBL contributes expertise in performing biochemical assays and clinical interpretation of the results. The CBL collaborates regularly with the Data Coordinating Center to provide expertise regarding laboratory medicine, optimizing GFR studies and generating GFR estimating equations, maintaining the Manual of Procedures, assuring the quality control of all assays, and providing continuity of biochemical assays through changes in instrumentation. The CBL participates in all Steering Committee meetings and conference calls and collaborates with investigators in the generation of abstracts, presentations, and manuscripts. The proposed aims of the CBL center on continuing to provide accurate, precise, and efficient measures of biochemical measurements of general kidney health and contribute as a scientific partner to further the research agenda of the CKiD Consortium. The CBL will continue to provide the laboratory kits for collection, handling, and transport of samples, accurately receive, process, and analyze these samples on a day-to-day basis, and provide timely data entry into Nephron. The CBL has established a new subcontract with the UMN ARDL that will assure accurate measurement of iohexol concentrations (and hence GFR) during this project period. Accordingly, the CBL will continue to develop and optimize GFR estimating equations using conventional biomarkers in Cohort 3 subjects and other participants with Stage 1 CKD, and in those having received a kidney transplant. The CBL has completed work on concept sheets showing that hyperuricemia is associated with more rapid renal progression and that microalbuminuria is no better predictor of renal progression than is urinary protein. The CBL will next examine beta trace protein to determine if it is useful in predicting GFR. The long term goal is to better characterize the CKiD population biochemically, maintain the integrity of the data, optimize the collection of specimens in a manner that maintains recruitment and retention of CKiD subjects, and generate relevant publications.