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Central Biochemistry Laboratory of the CKID Consortium

CKID联盟中央生化实验室

基本信息

批准号：
10454343
负责人：
GEORGE J SCHWARTZ
金额：
$ 35.88万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10454343
关键词：
Address Albumins Ancillary Study Archives Bicarbonates Biochemical Biochemistry Biological Assay Biological Markers Blood Blood specimen Calcium Cardiovascular Diseases Child Childhood Chronic Kidney Failure Clinical Collaborations Collection Creatinine Data Data Coordinating Center Databases Dry Ice Electrolytes Electronic Mail Equation Freezing Generations Glomerular Filtration Rate Glucose Goals Growth Hair Health Health Status Human Hyperuricemia Inflammation Instruction Iohexol Kidney Kidney Function Tests Kidney Transplantation Label Laboratories Lipids Manuals Manuscripts Measurement Measures Medicine Metabolism Microalbuminuria Minnesota Monitor Nail plate Nephrons Neurocognition Online Systems Participant Persons Phosphorus Plasma Population Procedures Process Productivity Prospective Studies Proteins Protocols documentation Publications Publishing Quality Control Reagent Reproducibility Research Research Personnel Risk Assessment Risk Factors Role Sampling Ships Site Specimen Subgroup Supervision Telephone Testing Time To specify Training Transplant Recipients Transportation Tube United States National Institutes of Health Universities Uric Acid Urine Vial device Visit Vitamin D Work base cardiovascular health case-by-case basis cathelicidin clinical research site cohort data integrity improved instrument instrumentation meetings post gamma-globulins preservation recruit repository sample collection skeletal symposium urinary

项目摘要

The Central Biochemistry Laboratory (CBL) serves the subjects and investigators of the continuing Prospective Study of Chronic Kidney Disease in Children (CKiD). The CBL provides participating clinical sites with protocols, kits, reagents, supplies, and transportation required for collecting blood and urine for analysis at URMC and other laboratories. Blood analytes include electrolytes, BUN, creatinine, glucose, calcium, phosphorus, albumin, uric acid, lipid screen, hsCRP, intact PTH, and cystatin C. Urine analytes include creatinine and protein. The CBL has determined optimal conditions for transporting these analytes, and provides ambient shippers for each visit and quarterly dry ice shippers for frozen specimens. The CBL also provides the kits and instructions for collection and shipment of blood, plasma, sera, urine, hair, and nail samples to NIH repositories. Biochemical data from each CKiD visit are reviewed and entered into Nephron, the CKiD data base. The CBL provides training at annual CKID meetings and is readily available to provide information, instruction, and support during CKiD visits. The CBL contributes expertise in performing biochemical assays and clinical interpretation of the results. The CBL collaborates regularly with the Data Coordinating Center to provide expertise regarding laboratory medicine, optimizing GFR studies and generating GFR estimating equations, maintaining the Manual of Procedures, assuring the quality control of all assays, and providing continuity of biochemical assays through changes in instrumentation. The CBL participates in all Steering Committee meetings and conference calls and collaborates with investigators in the generation of abstracts, presentations, and manuscripts. The proposed aims of the CBL center on continuing to provide accurate, precise, and efficient measures of biochemical measurements of general kidney health and contribute as a scientific partner to further the research agenda of the CKiD Consortium. The CBL will continue to provide the laboratory kits for collection, handling, and transport of samples, accurately receive, process, and analyze these samples on a day-to-day basis, and provide timely data entry into Nephron. The CBL has established a new subcontract with the UMN ARDL that will assure accurate measurement of iohexol concentrations (and hence GFR) during this project period. Accordingly, the CBL will continue to develop and optimize GFR estimating equations using conventional biomarkers in Cohort 3 subjects and other participants with Stage 1 CKD, and in those having received a kidney transplant. The CBL has completed work on concept sheets showing that hyperuricemia is associated with more rapid renal progression and that microalbuminuria is no better predictor of renal progression than is urinary protein. The CBL will next examine beta trace protein to determine if it is useful in predicting GFR. The long term goal is to better characterize the CKiD population biochemically, maintain the integrity of the data, optimize the collection of specimens in a manner that maintains recruitment and retention of CKiD subjects, and generate relevant publications.

中心生化实验室（CBL）为持续前瞻性研究的受试者和研究者提供服务。儿童慢性肾脏病（CKiD）研究。CBL为参与的临床研究中心提供收集血液和尿液进行分析所需的方案、试剂盒、试剂、供应品和运输， URMC和其他实验室。血液分析物包括电解质、BUN、肌酸酐、葡萄糖、钙，磷、白蛋白、尿酸、脂质筛查、hsCRP、完整PTH和胱抑素C。尿液分析物包括肌酸酐和蛋白质。CBL已经确定了运输这些分析物的最佳条件，并且为每次访视提供常温运输箱，为冷冻标本提供季度干冰运输箱。CBL还提供了血液、血浆、血清、尿液、毛发和指甲的采集和运输的试剂盒和说明样本送到NIH仓库。审查每次CKiD访视的生化数据并输入到Nephron， CKiD数据库。CBL在年度CKID会议上提供培训，并随时提供在CKiD访问期间提供信息、指导和支持。CBL提供专业知识，生物化学测定和结果的临床解释。CBL定期与数据合作协调中心提供有关实验室医学的专业知识，优化GFR研究，生成GFR估计方程，维护程序手册，确保所有分析，并通过仪器的变化提供生物化学分析的连续性。的CBL 参加所有指导委员会会议和电话会议，并与调查人员合作，生成摘要、演示文稿和手稿。CBL的拟议目标是继续提供一般肾脏健康的准确、精确和有效的生化测量指标并作为科学合作伙伴为进一步推动CKiD联盟的研究议程做出贡献。CBL将继续提供用于样本采集、处理和运输的实验室试剂盒，准确接收，每天处理和分析这些样本，并及时将数据输入到Nephron。的 CBL与UMN ARDL建立了一个新的校准器，以确保碘海醇的准确测量本项目期间的浓度（以及GFR）。因此，CBL将继续发展，在队列3受试者和其他受试者中使用常规生物标志物优化GFR估计方程 CKD 1期患者和接受肾移植的患者。CBL已完成概念设计工作显示高尿酸血症与更快的肾脏进展相关，并不比尿蛋白更好地预测肾脏进展。CBL接下来将检测β痕量蛋白以确定它是否有助于预测GFR。长期目标是更好地表征CKiD人群生物化学，保持数据的完整性，以一种维持CKiD受试者的招募和保留，并生成相关出版物。