Central Biochemistry Laboratory of the CKID Consortium

CKID联盟中央生化实验室

• 批准号: 10215266
• 负责人: GEORGE J SCHWARTZ
• 金额: $ 25.91万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10215266
• 关键词: Address; Albumins; Ancillary Study; Archives; Bicarbonates; Biochemical; Biochemistry; Biological Assay; Biological Markers; Blood; Blood specimen; Calcium; Cardiovascular Diseases; Child; Childhood; Chronic Kidney Failure; Clinical; Collaborations; Collection; Creatinine; Data; Data Coordinating Center; Databases; Dry Ice; Electrolytes; Electronic Mail; Equation; Freezing; Generations; Glomerular Filtration Rate; Glucose; Goals; Growth; Hair; Health; Health Status; Human; Hyperuricemia; Inflammation; Instruction; Iohexol; Kidney; Kidney Function Tests; Kidney Transplantation; Label; Laboratories; Lipids; Manuals; Manuscripts; Measurement; Measures; Medicine; Metabolism; Microalbuminuria; Minnesota; Monitor; Nail plate; Nephrons; Neurocognition; Online Systems; Participant; Persons; Phosphorus; Plasma; Population; Procedures; Process; Productivity; Prospective Studies; Proteins; Protocols documentation; Publications; Publishing; Quality Control; Reagent; Reproducibility; Research; Research Personnel; Risk Assessment; Risk Factors; Role; Sampling; Ships; Site; Specimen; Subgroup; Supervision; Telephone; Testing; Time; To specify; Training; Transplant Recipients; Transportation; Tube; United States National Institutes of Health; Universities; Uric Acid; Urine; Vial device; Visit; Vitamin D; Work; base; cardiovascular health; case-by-case basis; cathelicidin; clinical research site; cohort; data integrity; improved; instrument; instrumentation; meetings; post gamma-globulins; preservation; recruit; repository; sample collection; skeletal; symposium; urinary

The Central Biochemistry Laboratory (CBL) serves the subjects and investigators of the continuing Prospective Study of Chronic Kidney Disease in Children (CKiD). The CBL provides participating clinical sites with protocols, kits, reagents, supplies, and transportation required for collecting blood and urine for analysis at URMC and other laboratories. Blood analytes include electrolytes, BUN, creatinine, glucose, calcium, phosphorus, albumin, uric acid, lipid screen, hsCRP, intact PTH, and cystatin C. Urine analytes include creatinine and protein. The CBL has determined optimal conditions for transporting these analytes, and provides ambient shippers for each visit and quarterly dry ice shippers for frozen specimens. The CBL also provides the kits and instructions for collection and shipment of blood, plasma, sera, urine, hair, and nail samples to NIH repositories. Biochemical data from each CKiD visit are reviewed and entered into Nephron, the CKiD data base. The CBL provides training at annual CKID meetings and is readily available to provide information, instruction, and support during CKiD visits. The CBL contributes expertise in performing biochemical assays and clinical interpretation of the results. The CBL collaborates regularly with the Data Coordinating Center to provide expertise regarding laboratory medicine, optimizing GFR studies and generating GFR estimating equations, maintaining the Manual of Procedures, assuring the quality control of all assays, and providing continuity of biochemical assays through changes in instrumentation. The CBL participates in all Steering Committee meetings and conference calls and collaborates with investigators in the generation of abstracts, presentations, and manuscripts. The proposed aims of the CBL center on continuing to provide accurate, precise, and efficient measures of biochemical measurements of general kidney health and contribute as a scientific partner to further the research agenda of the CKiD Consortium. The CBL will continue to provide the laboratory kits for collection, handling, and transport of samples, accurately receive, process, and analyze these samples on a day-to-day basis, and provide timely data entry into Nephron. The CBL has established a new subcontract with the UMN ARDL that will assure accurate measurement of iohexol concentrations (and hence GFR) during this project period. Accordingly, the CBL will continue to develop and optimize GFR estimating equations using conventional biomarkers in Cohort 3 subjects and other participants with Stage 1 CKD, and in those having received a kidney transplant. The CBL has completed work on concept sheets showing that hyperuricemia is associated with more rapid renal progression and that microalbuminuria is no better predictor of renal progression than is urinary protein. The CBL will next examine beta trace protein to determine if it is useful in predicting GFR. The long term goal is to better characterize the CKiD population biochemically, maintain the integrity of the data, optimize the collection of specimens in a manner that maintains recruitment and retention of CKiD subjects, and generate relevant publications.

中央生物化学实验室 (CBL) 为持续的前瞻性研究的受试者和研究人员提供服务 儿童慢性肾脏病 (CKiD) 研究。 CBL 为参与临床中心提供 收集血液和尿液进行分析所需的方案、试剂盒、试剂、用品和运输 URMC 等实验室。血液分析物包括电解质、BUN、肌酐、葡萄糖、钙、 磷、白蛋白、尿酸、血脂筛查、hsCRP、完整 PTH 和胱抑素 C。尿液分析物包括 肌酐和蛋白质。 CBL 已确定运输这些分析物的最佳条件，并且 为每次访问提供常温托运人，并为冷冻标本提供季度干冰托运人。 CBL还 提供用于采集和运输血液、血浆、血清、尿液、头发和指甲的试剂盒和说明 样本到 NIH 存储库。每次 CKiD 就诊的生化数据都会经过审查并输入 Nephron， CKiD 数据库。 CBL 在年度 CKID 会议上提供培训，并且随时可以提供 CKiD 访问期间的信息、指导和支持。 CBL 贡献了执行方面的专业知识 生化测定和结果的临床解释。 CBL 定期与 Data 合作 协调中心提供有关实验室医学、优化 GFR 研究和 生成 GFR 估计方程，维护程序手册，确保所有的质量控制 测定，并通过仪器的变化提供生化测定的连续性。 CBL 参加所有指导委员会会议和电话会议，并与调查人员合作 摘要、演示文稿和手稿的生成。 CBL 中心的拟议目标是继续 提供准确、精确、高效的一般肾脏健康生化测量结果 作为科学合作伙伴，为推进 CKiD 联盟的研究议程做出贡献。 CBL 将 继续提供用于样本采集、处理和运输的实验室套件，准确接收、 每天处理和分析这些样本，并将数据及时输入 Nephron。这 CBL 与 UMN ARDL 建立了新的分包合同，以确保碘海醇的准确测量 本项目期间的浓度（以及 GFR）。因此，CBL 将继续发展和 使用第 3 组受试者和其他参与者中的传统生物标志物优化 GFR 估计方程 患有 1 期 CKD 的患者以及接受肾移植的患者。 CBL 已完成概念工作 表格显示高尿酸血症与更快的肾脏进展有关，并且微量白蛋白尿 与尿蛋白相比，它并不能更好地预测肾脏进展。 CBL 接下来将检查 β 痕量蛋白 以确定它是否有助于预测 GFR。长期目标是更好地描述 CKiD 人群的特征 生物化学，保持数据的完整性，以以下方式优化样本的收集： 维持 CKiD 受试者的招募和保留，并生成相关出版物。