Primary Human Airway Cell Culture Core

项目摘要/摘要 核心D细胞培养 本提案中描述的工作的目标是收集、生长、存储和扩增呼吸道上皮细胞 和平滑肌细胞。这些细胞将来自支气管镜活检(上皮刷检 平滑肌细胞和支气管镜活检)来自B芯的主要采集、生长 细胞的存储将在印第安纳大学进行，但CWRU将有一个卫星设施来培养细胞 克利夫兰的支气管镜检查。受试者将包括严重哮喘患者和健康对照。 研究对象。单元将被提供给来自两个地点的每个或项目的调查人员，以用于其目标， 如上所述。细胞将通过核心C的研究编号进行分类和标记(通过条形码)。这将 允许分析临床表型、药物反应、基因型(针对项目3)和 呼吸道细胞行为。细胞将长期储存在IU站点。每个样品都可以解冻，并进一步 在印第安纳大学现场进行了研究和测试。收集、扩展和分布呼吸道上皮细胞的能力 细胞对研究人员将对理解和开发常见疾病的治疗方法产生积极影响 呼吸道疾病。这将是这两个站点中唯一具有这些功能的核心。

PROJECT SUMMARY/ABSTRACT Core D Cell Culture The goal of the work described in this proposal is to collect grow, store and expand airway epithelial cells and smooth muscle cells. These cells will come from bronchoscopic biopsies (brush biopsies for epithelial cells and endobronchial biopsies for smooth muscle cells) from Core B. The main site for collecting, growing and storing the cells will be at IU, but there will be a satellite facility at CWRU to grow cells from bronchoscopies in Cleveland. Subjects will include both severe asthma patients and healthy control subjects. Cells will be provided to investigators from both sites to each or the Projects for use in their Aims, as described. Cells will be catalogued and labeled (by bar code) by study number from Core C. This will allow analysis of the relationship between clinical phenotype, drug response, genotype (for Project 3) and airway cell behavior. Cells will be stored long-term at the IU site. Each sample can be thawed and further expanded for study and testing at the IU site. The ability to collect, expand, and distribute airway epithelial cells to researchers will have a positive impact on understanding and developing therapies for common airway diseases. This will be the only core with these capabilities at either site.