Primary Human Airway Cell Culture Core

原代人气道细胞培养核心

• 批准号: 10269970
• 负责人: Michael D Davis
• 金额: $ 30.26万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10269970
• 关键词: 3-Dimensional; Acute; Air; Airway Disease; Asthma; Bar Codes; Biological Assay; Biopsy; Bronchoscopy; Cell Count; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Chronic; Collection; Cryopreservation; Cryopreserved Cell; D Cells; Disease; Doctor of Philosophy; Ensure; Epithelial; Epithelial Cells; Fibroblasts; Foundations; Functional disorder; Genotype; Goals; Gossypium; Growth; Harvest; Human; Indiana; Innate Immune System; Label; Liquid substance; Living Donors; Lung; Lung diseases; Maintenance; Methods; Morbidity - disease rate; Muscle Cells; National Heart, Lung, and Blood Institute; Patients; Pharmaceutical Preparations; Physiological; Play; Program Research Project Grants; Protocols documentation; Recording of previous events; Research Personnel; Rho-associated kinase; Role; Sampling; Site; Smooth Muscle; Smooth Muscle Myocytes; Source; Testing; Tissue Sample; Training; Universities; Work; airway epithelium; asthmatic patient; biophysical properties; cell behavior; cell repository; clinical phenotype; disorder control; experience; innovation; kinase inhibitor; mortality; muscle physiology; pathogen; patient population; repository; respiratory smooth muscle; response; therapy development

PROJECT SUMMARY/ABSTRACT Core D Cell Culture The goal of the work described in this proposal is to collect grow, store and expand airway epithelial cells and smooth muscle cells. These cells will come from bronchoscopic biopsies (brush biopsies for epithelial cells and endobronchial biopsies for smooth muscle cells) from Core B. The main site for collecting, growing and storing the cells will be at IU, but there will be a satellite facility at CWRU to grow cells from bronchoscopies in Cleveland. Subjects will include both severe asthma patients and healthy control subjects. Cells will be provided to investigators from both sites to each or the Projects for use in their Aims, as described. Cells will be catalogued and labeled (by bar code) by study number from Core C. This will allow analysis of the relationship between clinical phenotype, drug response, genotype (for Project 3) and airway cell behavior. Cells will be stored long-term at the IU site. Each sample can be thawed and further expanded for study and testing at the IU site. The ability to collect, expand, and distribute airway epithelial cells to researchers will have a positive impact on understanding and developing therapies for common airway diseases. This will be the only core with these capabilities at either site.

项目摘要/摘要 核D细胞培养 本提案中描述的工作的目的是收集生长，存储和扩展气道上皮细胞 和平滑肌细胞。这些细胞将来自支气管镜活检（上皮的刷活检 来自核心B的细胞和支撑型活检。 并且将单元存储在IU中，但是在CWRU中将有一个卫星设施，以从 克利夫兰的支气管镜。受试者将包括严重的哮喘患者和健康对照 主题。将向调查人员提供从两个站点到各个项目的调查人员，以供其目的使用， 如上所述。细胞将按照核心C的研究编号对细胞进行分类和标记（按条形码）。 允许分析临床表型，药物反应，基因型（项目3）和 气道细胞行为。细胞将长期存储在IU站点。每个样品可以解冻，然后进一步 扩展在IU站点进行研究和测试。收集，扩展和分发气道上皮的能力 研究人员的细胞将对理解和开发常见的疗法产生积极影响 气道疾病。这将是任何一个站点上这些功能的唯一核心。