Primary Human Airway Cell Culture Core

原代人气道细胞培养核心

• 批准号: 10662240
• 负责人: Michael D Davis
• 金额: $ 30.32万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10662240
• 关键词: 3-Dimensional; Acute; Air; Airway Disease; Asthma; Bar Codes; Biological Assay; Biopsy; Bronchoscopy; Cell Count; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Collection; Cryopreservation; Cryopreserved Cell; D Cells; Disease; Doctor of Philosophy; Ensure; Epithelial Cells; Epithelium; Fibroblasts; Foundations; Functional disorder; Genotype; Goals; Gossypium; Growth; Harvest; Human; Indiana; Innate Immune System; Label; Liquid substance; Living Donors; Lung; Lung diseases; Maintenance; Methods; Morbidity - disease rate; Muscle Cells; National Heart, Lung, and Blood Institute; Patients; Pharmaceutical Preparations; Physiological; Play; Program Research Project Grants; Protocols documentation; Recording of previous events; Research Personnel; Rho-associated kinase; Role; Sampling; Site; Smooth Muscle; Smooth Muscle Myocytes; Source; Testing; Tissues; Training; Universities; Work; airway epithelium; asthmatic patient; biophysical properties; cell behavior; cell repository; chronic respiratory disease; clinical phenotype; experience; experimental study; innovation; kinase inhibitor; mortality; muscle physiology; pathogen; patient population; programs; repository; respiratory smooth muscle; response; therapy development

PROJECT SUMMARY/ABSTRACT Core D Cell Culture The goal of the work described in this proposal is to collect grow, store and expand airway epithelial cells and smooth muscle cells. These cells will come from bronchoscopic biopsies (brush biopsies for epithelial cells and endobronchial biopsies for smooth muscle cells) from Core B. The main site for collecting, growing and storing the cells will be at IU, but there will be a satellite facility at CWRU to grow cells from bronchoscopies in Cleveland. Subjects will include both severe asthma patients and healthy control subjects. Cells will be provided to investigators from both sites to each or the Projects for use in their Aims, as described. Cells will be catalogued and labeled (by bar code) by study number from Core C. This will allow analysis of the relationship between clinical phenotype, drug response, genotype (for Project 3) and airway cell behavior. Cells will be stored long-term at the IU site. Each sample can be thawed and further expanded for study and testing at the IU site. The ability to collect, expand, and distribute airway epithelial cells to researchers will have a positive impact on understanding and developing therapies for common airway diseases. This will be the only core with these capabilities at either site.

项目总结/摘要 核心D细胞培养 本提案中描述的工作目标是收集、生长、储存和扩增气道上皮细胞 和平滑肌细胞。这些细胞将来自支气管镜活检（上皮细胞刷检）。 细胞和支气管内平滑肌细胞活检）。主要的网站为收集，种植， 储存细胞将在IU，但CWRU将有一个卫星设施， 在克利夫兰做支气管镜检查受试者将包括重度哮喘患者和健康对照 科目细胞将被提供给来自两个研究中心的研究者，以用于他们的目标， 如所描述的。将根据核心C的研究编号对细胞进行分类和标记（通过条形码）。这将 允许分析临床表型、药物应答、基因型（项目3）和 气道细胞行为细胞将长期储存在IU研究中心。每个样品都可以解冻， 在IU研究中心进行研究和检测。收集、扩张和分布气道上皮细胞的能力 研究人员的细胞将对理解和开发常见的治疗方法产生积极的影响。 呼吸道疾病这将是两个站点中唯一具有这些功能的核心。