Primary Human Airway Cell Culture Core

原代人气道细胞培养核心

• 批准号: 10662240
• 负责人: Michael D Davis
• 金额: $ 30.32万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10662240
• 关键词: 3-Dimensional; Acute; Air; Airway Disease; Asthma; Bar Codes; Biological Assay; Biopsy; Bronchoscopy; Cell Count; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Collection; Cryopreservation; Cryopreserved Cell; D Cells; Disease; Doctor of Philosophy; Ensure; Epithelial Cells; Epithelium; Fibroblasts; Foundations; Functional disorder; Genotype; Goals; Gossypium; Growth; Harvest; Human; Indiana; Innate Immune System; Label; Liquid substance; Living Donors; Lung; Lung diseases; Maintenance; Methods; Morbidity - disease rate; Muscle Cells; National Heart, Lung, and Blood Institute; Patients; Pharmaceutical Preparations; Physiological; Play; Program Research Project Grants; Protocols documentation; Recording of previous events; Research Personnel; Rho-associated kinase; Role; Sampling; Site; Smooth Muscle; Smooth Muscle Myocytes; Source; Testing; Tissues; Training; Universities; Work; airway epithelium; asthmatic patient; biophysical properties; cell behavior; cell repository; chronic respiratory disease; clinical phenotype; experience; experimental study; innovation; kinase inhibitor; mortality; muscle physiology; pathogen; patient population; programs; repository; respiratory smooth muscle; response; therapy development

PROJECT SUMMARY/ABSTRACT Core D Cell Culture The goal of the work described in this proposal is to collect grow, store and expand airway epithelial cells and smooth muscle cells. These cells will come from bronchoscopic biopsies (brush biopsies for epithelial cells and endobronchial biopsies for smooth muscle cells) from Core B. The main site for collecting, growing and storing the cells will be at IU, but there will be a satellite facility at CWRU to grow cells from bronchoscopies in Cleveland. Subjects will include both severe asthma patients and healthy control subjects. Cells will be provided to investigators from both sites to each or the Projects for use in their Aims, as described. Cells will be catalogued and labeled (by bar code) by study number from Core C. This will allow analysis of the relationship between clinical phenotype, drug response, genotype (for Project 3) and airway cell behavior. Cells will be stored long-term at the IU site. Each sample can be thawed and further expanded for study and testing at the IU site. The ability to collect, expand, and distribute airway epithelial cells to researchers will have a positive impact on understanding and developing therapies for common airway diseases. This will be the only core with these capabilities at either site.

项目概要/摘要 核心 D 细胞培养 本提案中描述的工作目标是收集生长、储存和扩展气道上皮细胞 和平滑肌细胞。这些细胞将来自支气管镜活检（上皮细胞刷活检） 细胞和平滑肌细胞的支气管内活检）来自 Core B。收集、生长的主要场所 细胞的储存将在印第安纳大学，但在 CWRU 将有一个卫星设施来培养细胞 克利夫兰的支气管镜检查。受试者将包括严重哮喘患者和健康对照者 科目。细胞将被提供给来自两个地点或每个项目的研究人员，以用于他们的目标， 正如所描述的。细胞将按照 Core C 的研究编号进行编目和标记（通过条形码）。这将 允许分析临床表型、药物反应、基因型（针对项目 3）和 气道细胞行为。细胞将长期保存在 IU 站点。每个样品都可以解冻并进一步 扩大到印第安纳大学网站进行研究和测试。收集、扩张和分布气道上皮的能力 细胞对研究人员来说将对理解和开发常见的治疗方法产生积极影响 气道疾病。这将是这两个站点中唯一具有这些功能的核心。