Primary Human Airway Cell Culture Core
原代人气道细胞培养核心
基本信息
- 批准号:10662240
- 负责人:
- 金额:$ 30.32万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-01 至 2026-04-30
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAcuteAirAirway DiseaseAsthmaBar CodesBiological AssayBiopsyBronchoscopyCell CountCell Culture TechniquesCell LineCell SeparationCellsCollectionCryopreservationCryopreserved CellD CellsDiseaseDoctor of PhilosophyEnsureEpithelial CellsEpitheliumFibroblastsFoundationsFunctional disorderGenotypeGoalsGossypiumGrowthHarvestHumanIndianaInnate Immune SystemLabelLiquid substanceLiving DonorsLungLung diseasesMaintenanceMethodsMorbidity - disease rateMuscle CellsNational Heart, Lung, and Blood InstitutePatientsPharmaceutical PreparationsPhysiologicalPlayProgram Research Project GrantsProtocols documentationRecording of previous eventsResearch PersonnelRho-associated kinaseRoleSamplingSiteSmooth MuscleSmooth Muscle MyocytesSourceTestingTissuesTrainingUniversitiesWorkairway epitheliumasthmatic patientbiophysical propertiescell behaviorcell repositorychronic respiratory diseaseclinical phenotypeexperienceexperimental studyinnovationkinase inhibitormortalitymuscle physiologypathogenpatient populationprogramsrepositoryrespiratory smooth muscleresponsetherapy development
项目摘要
PROJECT SUMMARY/ABSTRACT
Core D Cell Culture
The goal of the work described in this proposal is to collect grow, store and expand airway epithelial cells
and smooth muscle cells. These cells will come from bronchoscopic biopsies (brush biopsies for epithelial
cells and endobronchial biopsies for smooth muscle cells) from Core B. The main site for collecting, growing
and storing the cells will be at IU, but there will be a satellite facility at CWRU to grow cells from
bronchoscopies in Cleveland. Subjects will include both severe asthma patients and healthy control
subjects. Cells will be provided to investigators from both sites to each or the Projects for use in their Aims,
as described. Cells will be catalogued and labeled (by bar code) by study number from Core C. This will
allow analysis of the relationship between clinical phenotype, drug response, genotype (for Project 3) and
airway cell behavior. Cells will be stored long-term at the IU site. Each sample can be thawed and further
expanded for study and testing at the IU site. The ability to collect, expand, and distribute airway epithelial
cells to researchers will have a positive impact on understanding and developing therapies for common
airway diseases. This will be the only core with these capabilities at either site.
项目概要/摘要
核心 D 细胞培养
本提案中描述的工作目标是收集生长、储存和扩展气道上皮细胞
和平滑肌细胞。这些细胞将来自支气管镜活检(上皮细胞刷活检)
细胞和平滑肌细胞的支气管内活检)来自 Core B。收集、生长的主要场所
细胞的储存将在印第安纳大学,但在 CWRU 将有一个卫星设施来培养细胞
克利夫兰的支气管镜检查。受试者将包括严重哮喘患者和健康对照者
科目。细胞将被提供给来自两个地点或每个项目的研究人员,以用于他们的目标,
正如所描述的。细胞将按照 Core C 的研究编号进行编目和标记(通过条形码)。这将
允许分析临床表型、药物反应、基因型(针对项目 3)和
气道细胞行为。细胞将长期保存在 IU 站点。每个样品都可以解冻并进一步
扩大到印第安纳大学网站进行研究和测试。收集、扩张和分布气道上皮的能力
细胞对研究人员来说将对理解和开发常见的治疗方法产生积极影响
气道疾病。这将是这两个站点中唯一具有这些功能的核心。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael D Davis其他文献
Tissue factor expression, regulation, and signaling in human airway cells
人气道细胞中的组织因子表达、调节和信号传导
- DOI:
10.25772/2n5p-2z33 - 发表时间:
2017 - 期刊:
- 影响因子:4.9
- 作者:
Michael D Davis - 通讯作者:
Michael D Davis
2019 Year in Review: Asthma
2019 年回顾:哮喘
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:2.5
- 作者:
Michael D Davis;Craig D Smallwood - 通讯作者:
Craig D Smallwood
Blood Gas Analysis and Hemoximetry 2013
血气分析和血氧测定法 2013
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
Michael D Davis;Brian K. Walsh;Ruben D. Restrepo - 通讯作者:
Ruben D. Restrepo
The remaining barriers to normalcy in CF: Advances in assessment of CF lung disease
CF 正常化的剩余障碍:CF 肺部疾病评估的进展
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:3.1
- 作者:
Heather N Muston;L. Perrem;Michael D Davis;F. Ratjen;C. Ren - 通讯作者:
C. Ren
Real-Time Iterative Monitoring of Radiofrequency Ablation Tumor Therapy with 15O-Water PET Imaging
使用 15O-水 PET 成像实时迭代监测射频消融肿瘤治疗
- DOI:
10.2967/jnumed.108.052886 - 发表时间:
2008 - 期刊:
- 影响因子:9.3
- 作者:
A. Bao;B. Goins;G. Dodd;A. Soundararajan;Cristina Santoyo;R. Otto;Michael D Davis;W. Phillips - 通讯作者:
W. Phillips
Michael D Davis的其他文献
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