Single Cell Characterization and Procurement Core

SCCPC摘要 作为我们在单细胞水平上分析造血细胞的直接结果，我们的理解 在过去的10年里，造血系统的复杂性和异质性发生了戏剧性的演变 好几年了。高端流式细胞术和转录学的使用使研究人员能够测量细胞与细胞之间的 细胞差异和基因组信息与单细胞功能相关。我们对非的理解 恶性血液病正在迅速演变，现在需要整合转录组、表观基因组、 蛋白质组(和现在的“细胞组”)既在单细胞水平上，也在骨髓中原位。然而， 严格的造血干和祖细胞的实验解剖(HSCP)需要专门的 专业知识和非凡的技术敏捷性是该领域许多研究人员的障碍。回答 这一关键需求，即单一细胞鉴定和采购核心(SCCPC)旨在提供 数据分析平台方面的专业知识以及专业服务的技术建议(和访问权限) 促进尖端的多维单细胞功能和机械分析 对非恶性血液病的认识。尽管这类技术广泛可用，但访问 大多数机构的这些服务并不伴随着对造血和 干细胞生物学(和/或分析管道)。因此，有必要方便地获得培训和 在使用分析平台以及如何集成数据集方面的专业知识。SCCPC提供了独特的 在单细胞一级研究造血的服务包括：(1)标准化和集中化 在流式细胞仪分析和分离方面提供服务，以降低调查人员的成本；(2)专业服务 在多光谱流式细胞术和多维分析中；(3)专门的分析管道 整合正交层；(4)高分辨率共聚焦的专业化服务 固定、活体和原位单细胞成像的免疫荧光成像；(5)专业服务 研究设计、大数据集分析和解释；以及(6)开发新的前沿 供研究人员在研究中使用的技术。SCCPC的中心目标是创建一个 集中式中心提供有关数据分析和集成的建议/专业知识，以促进 全面的尖端多维单细胞理解-从功能和 从机制上讲--非恶性血液病。SCCPC拥有受过专业培训的工作人员 具有丰富的科学实验设计和客户支持经验。核心的目标是 提供创新、最先进的设施和尖端的技术援助，以实现高质量 单个造血细胞的分析；提供统一质量的细胞制剂；降低 使用共享的流式细胞术、基因组分析和成像设备进行个人调查；以及 就细胞分析、分离、生物信息学分析和成像方面的新概念进行培训/教育。

SCCPC Abstract As a direct result of our ability to analyze hematopoietic cells at the single cell level, our understanding of the complexity and heterogeneity of the hematopoietic system has dramatically evolved in the past 10 years. The use of high-end flow cytometry and transcriptomics allowed investigators to measure cell-to- cell differences and correlate genomic information with single-cell function. Our understanding of non- malignant hematology is rapidly evolving, and now requires integration of transcriptome, epigenome, proteome (and now “organellome”) both at the single-cell level and in bone marrow in situ. However, rigorous experimental dissection of hematopoietic stem and progenitors (HSCP) requires specialized expertise and fascile technical agility that serves as a barrier to many researchers in the field. To answer this critical need, the Single Cell Characterization and Procurement Core (SCCPC) is designed to provide both expertise on data analytics platforms as well as technical advise (and access to) specialized services to facilitate cutting-edge multi-dimensional single-cell analyses for functional and mechanistic understanding of non-malignant hematology. Although such technologies are widely available, access to these services at most institutions is not accompanied by requisite understanding of hematopoiesis and stem cell biology (and/or analysis pipelines). Thus, there is a need for easy access to training and expertise on using analytic platforms, and how to integrate datasets. The SCCPC provides unique services for investigating hematopoiesis at the single-cell level including: (1) standardized and centralized services in flow cytometry analysis and isolation to reduce costs for investigators; (2) specialized services in multispectral flow cytometry and multi-dimensional analyses; (3) specialized analysis pipelines for integrating orthogonal “omics” layers; (4) specialized services in high resolution confocal immunofluorescence imaging for fixed, live and in situ single cell imaging; (5) specialized services in study design, large dataset analyses and interpretation; and (6) development of new cutting-edge technologies for investigators to use in their research. The Central Goal of the SCCPC is to create a centralized center providing advise/expertise on data analyses and integration to facilitate a comprehensive cutting-edge multi-dimensional single-cell understanding – functionally and mechanistically – of non-malignant hematology. The SCCPC has dedicated professionally trained staff with great experience in scientific experimental design and client support. The aims of the core are to provide innovative, state-of-the-art facilities and sophisticated technical assistance for high quality analysis of single hematopoietic cells; to provide uniform quality of cell preparations; to reduce costs of individual investigators by using shared flow cytometry, genomics analyses and imaging facilities; and to train/educate in new concepts on cell analysis, isolation, bioinformatics analyses and imaging.