Single Cell Characterization and Procurement Core

SCCPC摘要 由于我们能够在单细胞水平上分析造血细胞， 造血系统的复杂性和异质性在过去10年中发生了巨大的变化， 年高端流式细胞术和转录组学的使用使研究人员能够测量细胞与细胞间的 细胞差异，并将基因组信息与单细胞功能相关联。我们对非- 恶性血液学正在迅速发展，现在需要整合转录组，表观基因组， 蛋白质组（现在称为“细胞器组”）在单细胞水平和骨髓原位。然而，在这方面， 造血干细胞和祖细胞（HSCP）的严格实验解剖需要专门的 专业知识和快速的技术敏捷性是该领域许多研究人员的障碍。回答 为了满足这一关键需求，单细胞表征和采购核心（SCCPC）旨在提供 数据分析平台的专业知识以及技术建议（和访问）专业服务 以促进尖端的多维单细胞分析， 了解非恶性血液学。虽然这些技术可以广泛获得， 大多数机构的这些服务没有伴随着对造血的必要理解， 干细胞生物学（和/或分析管道）。因此，有必要提供方便的培训机会， 使用分析平台的专业知识，以及如何集成数据集。SCCPC提供独特的 在单细胞水平上研究造血的服务包括：（1）标准化和集中化 流式细胞仪分析和分离服务，以降低研究人员的成本;（2）专业服务 在多光谱流式细胞术和多维分析中;（3）专门的分析管道， 整合正交的“组学”层;（4）高分辨率共聚焦显微镜中的专业服务 免疫荧光成像，用于固定、活体和原位单细胞成像;（5） 研究设计、大型数据集分析和解释;以及（6）开发新的尖端技术 研究人员在研究中使用的技术。党中央的中心任务是创建一个 集中式中心，提供有关数据分析和集成的建议/专业知识， 全面的尖端多维单细胞理解-功能和 非恶性血液学的机制。SCCPC拥有专门的专业培训人员 在科学实验设计和客户支持方面拥有丰富的经验。核心的目标是 提供创新的，最先进的设施和先进的技术援助， 单个造血细胞的分析;提供统一质量的细胞制剂;降低 通过使用共享的流式细胞术、基因组学分析和成像设施， 在细胞分析、分离、生物信息学分析和成像方面进行新概念的培训/教育。

SCCPC Abstract As a direct result of our ability to analyze hematopoietic cells at the single cell level, our understanding of the complexity and heterogeneity of the hematopoietic system has dramatically evolved in the past 10 years. The use of high-end flow cytometry and transcriptomics allowed investigators to measure cell-to- cell differences and correlate genomic information with single-cell function. Our understanding of non- malignant hematology is rapidly evolving, and now requires integration of transcriptome, epigenome, proteome (and now “organellome”) both at the single-cell level and in bone marrow in situ. However, rigorous experimental dissection of hematopoietic stem and progenitors (HSCP) requires specialized expertise and fascile technical agility that serves as a barrier to many researchers in the field. To answer this critical need, the Single Cell Characterization and Procurement Core (SCCPC) is designed to provide both expertise on data analytics platforms as well as technical advise (and access to) specialized services to facilitate cutting-edge multi-dimensional single-cell analyses for functional and mechanistic understanding of non-malignant hematology. Although such technologies are widely available, access to these services at most institutions is not accompanied by requisite understanding of hematopoiesis and stem cell biology (and/or analysis pipelines). Thus, there is a need for easy access to training and expertise on using analytic platforms, and how to integrate datasets. The SCCPC provides unique services for investigating hematopoiesis at the single-cell level including: (1) standardized and centralized services in flow cytometry analysis and isolation to reduce costs for investigators; (2) specialized services in multispectral flow cytometry and multi-dimensional analyses; (3) specialized analysis pipelines for integrating orthogonal “omics” layers; (4) specialized services in high resolution confocal immunofluorescence imaging for fixed, live and in situ single cell imaging; (5) specialized services in study design, large dataset analyses and interpretation; and (6) development of new cutting-edge technologies for investigators to use in their research. The Central Goal of the SCCPC is to create a centralized center providing advise/expertise on data analyses and integration to facilitate a comprehensive cutting-edge multi-dimensional single-cell understanding – functionally and mechanistically – of non-malignant hematology. The SCCPC has dedicated professionally trained staff with great experience in scientific experimental design and client support. The aims of the core are to provide innovative, state-of-the-art facilities and sophisticated technical assistance for high quality analysis of single hematopoietic cells; to provide uniform quality of cell preparations; to reduce costs of individual investigators by using shared flow cytometry, genomics analyses and imaging facilities; and to train/educate in new concepts on cell analysis, isolation, bioinformatics analyses and imaging.