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Role of mTORC1 in Retinal Ganglion Cell Physiology and Disease

mTORC1 在视网膜神经节细胞生理和疾病中的作用

基本信息

批准号：
10458570
负责人：
Steven F Abcouwer
金额：
$ 50.64万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10458570
关键词：
Address Affect Binding Proteins Biological Assay Brain Cause of Death Cell Size Cell Survival Cell physiology Cells Cellular Metabolic Process Cellular Stress Characteristics Clinical Complex DNA Damage Data Dendrites Development Diabetes Mellitus Diabetic Retinopathy Disease Enterobacteria phage P1 Cre recombinase Exhibits FRAP1 gene Functional disorder Ganglion Cell Layer Genes Genetic Translation Glaucoma Goals Growth Growth Factor In Situ Knowledge Label Maintenance Mediating Messenger RNA Metabolic Metabolism Multiprotein Complexes Mus Nerve Degeneration Nerve Fibers Neural Retina Neurons Neuropathy Nutrient Pathway interactions Peptide Initiation Factors Phosphotransferases Physiology Play Predisposition Protein Biosynthesis Proteins Raptors Retina Retinal Diseases Retinal Ganglion Cells RiboTag Role Signal Pathway Signal Transduction Stress Synaptic plasticity Testing Thinness Time Tissues Translating Translations Tuberous Sclerosis Up-Regulation Vision Work adeno-associated viral vector axon guidance base cell growth cell motility cell regeneration conditional knockout diabetic diabetic patient experimental study ganglion cell in vivo inhibitor innovation mTOR Inhibitor mTOR inhibition method development negative affect nervous system disorder novel prevent protein expression response retinal neuron translatome

项目摘要

PROJECT SUMMARY This work addresses a fundamental knowledge gap in our understanding of retinal physiology and function that has significant bearing on the early effects of diabetes on the neural retina. Mechanistic target of rapamycin (mTOR) kinase forms the core of two multi-protein complexes: mTOR complex 1 (mTORC1) containing the protein Raptor, and mTORC2 complex containing the protein Rictor. The mTOR signaling network is essential for cellular responses to trophic signals, control of cell metabolism, protein synthesis, cell growth and cell motility. Numerous studies show a key role for mTOR complexes in neuronal function, including axon guidance, dendrite arborization and synaptic plasticity; and numerous neurological disorders are associated with dysfunctions of the mTOR signaling pathway. In contrast, knowledge of the roles of mTOR complexes in retinal physiology and disease is very limited. We propose to test a distinct cell-specific role for mTORC1 in normal retinal ganglion cell (RGC) physiology and to determine if loss of mTORC1 activity is a key contributor to loss of RGC function and viability in diabetes. The proposed study is based on our prior findings that diabetes causes progressive loss of total retinal protein synthesis (Fort, P.E. et al. 2014, Diabetes 63(9):3077-90) and preliminary data showing that: 1) mouse RGC exhibit a high rate of protein synthesis that is dependent upon mTORC1 function, and 2) negating mTORC1 function in the inner retina caused eventual loss of RGC, similar to the neurodegeneration causes by diabetes. mTORC1 activity is required for 5' cap- dependent translation of mRNAs encoding the protein-synthetic machinery. Thus, in Specific Aim 1 we plan to examine the role of mTORC1 activity in RGC protein synthesis and maintenance of RGC function and viability. We will test the hypothesis that loss of mTORC1 function in RGC inhibits translation of a discrete set of mRNAs, eventually leading to a decrease in the general protein synthetic capacity, visual function and viability of RGC. The proposal is also based upon the premise that diabetes causes stress and damage to the neural retina, and RGC in particular. Deactivation of mTORC1 decreases 5' cap-dependent protein translation in response to a number of cellular stresses. Preliminary data also show that diabetes diminishes RGC protein synthesis coinciding with increased expression of the stress-responsive inhibitor of mTORC1 called regulated in development and DNA damage (REDD1). Thus, in Specific Aim 2 we plan to determine if the effects of diabetes on RGC mRNA translation, function and viability are due to lack of mTORC1 activity leading to a reduction in protein synthesis capacity. We will test the hypothesis that maintaining mTORC1 activity and RGC protein translation during diabetes prevents RGC loss and dysfunction. Defining the role of mTORC1 in RGC will greatly increase our knowledge of RGC physiology and of the ways in which diabetes affects the neural retina.