Scientific Human Biomarker Exposure Monitoring Core (HEMC) for the Air pollution disrupts Inflammasome Regulation in HEart And Lung Total Health (AIRHEALTH) Study

摘要：人体生物标志物暴露监测核心 (HEMC) 科学人类生物标志物暴露监测核心 (HEMC) 是位于 斯坦福大学医学院建立在斯坦福大学人体免疫监测的丰富经验之上 核心实验室（HIMC）。 HEMC 将通过执行全面的、 基于免疫的标准化细胞培养测定和心血管测定。 HEMC 的总体目标 管理和处理生物标志物样本，定义污染物的多维生物标志物概况 暴露，并提供与污染物暴露相关的表观修饰的最先进的测定。的 HEMC 进行的实验结果将成为该 PPG 各个项目的核心 为各个项目提供所需的样本，并指导使用信息最丰富的样本 机制和模型研究。为此，我们的三个具体目标是： 目标 1. 空气污染中央银行 队列血浆、外周血单核细胞 (PBMC)、RNA 和 DNA，并提供这些样本 向 PPG 项目提供标准化和定制化验；目标 2. 提供标准化的针对性 蛋白质组学和基因表达分析有助于表征污染暴露特征 (PES) 将补充 PPG 的具体项目；目标 3. 提供标准化、创新的 表观遗传学分析可增强所有项目的机械发现。 HEMC 汇集了无与伦比的组合 斯坦福大学炎症体监测和机械工作技术。我们给予了细心和 系统地思考选择要进行的测定，以评估污染物的不同方面 曝光。 HEMC 中使用的许多技术主要是在斯坦福大学和 经斯坦福大学以外的机构验证，增加了我们对其应用于 PPG 项目的信心。在 HEMC 将与 DMAC 合作，为拟议的 PPG 提供创新工具和支持，以实现 分析分子机制并寻找防治污染的治疗靶点 相关的心肺风险，如项目 1、2 和 3 中提出的。

ABSTRACT: HUMAN BIOMARKER EXPOSURE MONITORING CORE (HEMC) The Scientific Human Biomarker Exposure Monitoring Core (HEMC) is a state-of-the-art Core Laboratory at Stanford School of Medicine that builds on the extensive experience of the Stanford Human Immune Monitoring Core Laboratory (HIMC). The HEMC will serve the aims of Projects 1, 2 and 3 by performing comprehensive, standardized immune-based cell culture assays, and cardiovascular assays. The overarching goals of the HEMC are to manage and process biomarker samples, define the multidimension biomarker profile of pollutant exposure, and to provide state of the art assays for epigenic modifications relevant to pollutant exposure. The results of the experiments performed by the HEMC will be central to the individual projects of this PPG by providing needed samples to the individual projects and guiding the use of the most informative samples for mechanistic and modeling studies. To this end, our three specific aims are: Aim 1. To centrally bank air pollution cohort plasma, peripheral blood mononuclear cells (PBMCs), RNA, and DNA, and to provide those specimens to the PPG Projects for both standardized and customized assays; Aim 2. To provide standardized targeted proteomics and gene expression assays instrumental for characterizing pollution exposure signature (PES) that will be complimentary to the specific projects of the PPG; and Aim 3. To provide a standardized, innovative epigenetic assay to enhance mechanistic findings in all projects. The HEMC brings together an unparalleled set of Stanford technologies for inflammasome monitoring and mechanistic work. We have given careful and systematic thought to the selection of assays to be performed that will evaluate different aspects of pollutant exposure. Many of technologies used in the HEMC have been largely developed and optimized at Stanford and validated by institutions outside Stanford, increasing our confidence for their application to the PPG projects. In collaboration with the DMAC, the HEMC will offer innovative tools and support for this proposed PPG to enable analysis of molecular mechanisms and to find therapeutic targets for the prevention and treatment of pollution- related cardiopulmonary risks, as proposed in Projects 1, 2, and 3.