Scientific Human Biomarker Exposure Monitoring Core (HEMC) for the Air pollution disrupts Inflammasome Regulation in HEart And Lung Total Health (AIRHEALTH) Study

摘要：人体生物标志物暴露监测核心(HEMC) 科学人类生物标志物暴露监测核心(HEMC)是一个最先进的核心实验室，位于 斯坦福大学医学院，以斯坦福大学人类免疫监测的丰富经验为基础 核心实验室(HIMC)。HEMC将服务于项目1、2和3的目标， 标准化的基于免疫的细胞培养检测和心血管检测。HEMC的总体目标 管理和处理生物标记物样本，定义污染物的多维生物标记物概况 并为与污染物暴露相关的表观修饰提供最先进的分析方法。这个 HEMC进行的实验结果将是该PPG个别项目的核心，通过 为各个项目提供所需的样本，并指导使用信息最丰富的样本 机构学和模型学。为此，我们的三个具体目标是：目标1.集中治理空气污染 收集血浆、外周血单核细胞(PBMC)、RNA和DNA，并提供这些标本 向PPG项目提供标准化和定制化验；目标2.提供标准化的、有针对性的 蛋白质组学和基因表达分析有助于表征污染暴露特征(PES) 将与PPG的具体项目相辅相成；和目标3.提供标准化、创新的 表观遗传学分析，以增强所有项目中的机械性发现。HEMC汇集了一套无与伦比的产品 斯坦福大学用于炎症监测和机械工作的技术。我们已经给予了谨慎和 选择评价污染物不同方面的分析方法的系统思考 曝光。HEMC中使用的许多技术在很大程度上是由斯坦福大学和 经过斯坦福大学以外的机构的验证，增加了我们对它们应用于PPG项目的信心。在……里面 与DMAC合作，HEMC将为这一提议的PPG提供创新的工具和支持，以实现 分析分子机制，寻找防治污染的治疗靶点- 相关的心肺风险，如项目1、2和3所述。

ABSTRACT: HUMAN BIOMARKER EXPOSURE MONITORING CORE (HEMC) The Scientific Human Biomarker Exposure Monitoring Core (HEMC) is a state-of-the-art Core Laboratory at Stanford School of Medicine that builds on the extensive experience of the Stanford Human Immune Monitoring Core Laboratory (HIMC). The HEMC will serve the aims of Projects 1, 2 and 3 by performing comprehensive, standardized immune-based cell culture assays, and cardiovascular assays. The overarching goals of the HEMC are to manage and process biomarker samples, define the multidimension biomarker profile of pollutant exposure, and to provide state of the art assays for epigenic modifications relevant to pollutant exposure. The results of the experiments performed by the HEMC will be central to the individual projects of this PPG by providing needed samples to the individual projects and guiding the use of the most informative samples for mechanistic and modeling studies. To this end, our three specific aims are: Aim 1. To centrally bank air pollution cohort plasma, peripheral blood mononuclear cells (PBMCs), RNA, and DNA, and to provide those specimens to the PPG Projects for both standardized and customized assays; Aim 2. To provide standardized targeted proteomics and gene expression assays instrumental for characterizing pollution exposure signature (PES) that will be complimentary to the specific projects of the PPG; and Aim 3. To provide a standardized, innovative epigenetic assay to enhance mechanistic findings in all projects. The HEMC brings together an unparalleled set of Stanford technologies for inflammasome monitoring and mechanistic work. We have given careful and systematic thought to the selection of assays to be performed that will evaluate different aspects of pollutant exposure. Many of technologies used in the HEMC have been largely developed and optimized at Stanford and validated by institutions outside Stanford, increasing our confidence for their application to the PPG projects. In collaboration with the DMAC, the HEMC will offer innovative tools and support for this proposed PPG to enable analysis of molecular mechanisms and to find therapeutic targets for the prevention and treatment of pollution- related cardiopulmonary risks, as proposed in Projects 1, 2, and 3.