Analytic Core

分析核心

• 批准号: 10460364
• 负责人: Danyelle M. Townsend
• 金额: $ 27.18万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10460364
• 关键词: Amino Acids; Analytical Biochemistry; Antioxidants; Biochemistry; Bioenergetics; Biological Assay; Biology; Centers of Research Excellence; Communities; Complement; Computer software; Consultations; Coupling; Detection; Development; Ensure; Enzyme Kinetics; Enzymes; Equilibrium; Equipment; Event; Extravasation; Fatty Acids; Flow Cytometry; Fluorescence Resonance Energy Transfer; Generations; Genus Hippocampus; Glutathione Disulfide; Goals; Grant; Homeostasis; Human Pathology; Human Resources; In Situ; Infrastructure; International; Kinetics; Label; Leadership; Manuscripts; Measurement; Measures; Mediating; Membrane Proteins; Mentors; Metabolic; Methods; Mitochondria; Oxidants; Oxidation-Reduction; Oxygen Consumption; Pathway Analysis; Phase; Phosphorylation; Post-Translational Protein Processing; Process; Protein Analysis; Protein Glycosylation; Protein S; Proteins; Proteomics; Protons; Qualitative Methods; Reaction; Research; Research Personnel; Resolution; Resources; Services; Signal Pathway; Signal Transduction; Site; South Carolina; Stress; Sulfhydryl Compounds; Techniques; Technology; Time; Tissues; Training; Training and Education; Writing; antioxidant enzyme; base; biological systems; design; enzyme activity; extracellular; human disease; in silico; innovation; instrument; interdisciplinary approach; member; millisecond; molecular modeling; multidisciplinary; nitrosative stress; oxidant stress; oxidation; protein protein interaction; skills; sugar

Analytical Redox Core – Project Summary The South Carolina COBRE in Oxidants, Redox Balance and Stress Signaling (Redox COBRE) Analytical Redox Core (ARC) offers infrastructure, expertise and training to Center members to support the successful application of quantitative techniques for the study of redox homeostasis and cellular bioenergetics. This includes unique approaches, technologies and equipment specific to the detection of redox-sensitive molecules and enzymes that drive redox homeostasis. The aims of the ARC are to: provide investigators with advanced capabilities in quantitative analytical redox biochemistry; state-of-the-art bioenergetic profiling; analysis and characterization of redox signaling pathways; and in particular, expert mentoring, education, training and consultation with COBRE personnel are top priorities of the ARC. Thus, we are able to impart the importance of redox signaling pathways in human disease, ensuring ongoing Core utilization and development of our user base, while enabling scientific discovery by COBRE investigators and the greater MUSC research community. During Phase III, we will integrate key resources (e.g., Seahorse XF bioenergetics profiling assays) previously available through the Bioenergetics Core that is now no longer available. Combined with our expertise in analytical biochemistry, the Phase III ARC aims to provide COBRE members with a multidisciplinary and comprehensive suite of services for quantifying ROS/RNS species and redox-modified proteins as well as characterization of their impact on bioenergetic networks and pathobiological processes.

分析氧化还原核心-项目摘要 南卡罗来纳州COBRE在氧化剂，氧化还原平衡和压力信号（氧化还原COBRE）分析氧化还原 核心（ARC）为中心成员提供基础设施，专业知识和培训，以支持成功的应用 氧化还原稳态和细胞生物能量学研究的定量技术。其中包括独特的 专门用于检测氧化还原敏感分子和酶的方法、技术和设备 来维持氧化还原平衡ARC的目标是：为调查人员提供先进的能力， 定量分析氧化还原生物化学;最先进的生物能量分析;分析和表征 氧化还原信号通路;特别是与COBRE的专家指导、教育、培训和咨询 人员是ARC的首要任务。因此，我们能够赋予氧化还原信号通路的重要性， 在人类疾病中，确保我们的用户群的持续核心利用和发展，同时使科学 COBRE调查人员和更大的MUSC研究社区的发现。在第三阶段，我们将 整合关键资源（例如，Seahorse XF生物能量学分析测定）， 生物能量学核心现在不再可用。结合我们在分析生物化学方面的专业知识， 第三阶段ARC旨在为COBRE成员提供多学科和全面的服务套件 用于量化活性氧/RNS物质和氧化还原修饰的蛋白质以及表征其对 生物能量网络和病理生物学过程。