Appalachian Center for Cellular transport in Obesity Related Disorders (ACCORD) Marshall University Imaging Core

阿巴拉契亚肥胖相关疾病细胞运输中心 (ACCORD) 马歇尔大学影像核心

• 批准号: 10460404
• 负责人: Michael Louis Norton
• 金额: $ 8.02万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10460404
• 关键词: Address; Affect; Appalachian Region; Atomic Force Microscopy; Award; Binding Proteins; Biological Models; Biomedical Research; Cells; Centers of Research Excellence; Communities; Complex; Consult; Cues; Custom; Development; Disease; Electrons; Elements; Energy Transfer; Evolution; Experimental Designs; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Foundations; Funding; Future; Generations; Goals; Growth; Health; Hypertension; Image; Image Analysis; Imaging Device; Imaging Techniques; Imaging technology; Individual; Institutes; Interdisciplinary Study; Kinetics; Letters; Malignant Neoplasms; Manuscripts; Methods; Molecular; Monitor; Obesity; Obesity associated disease; Optics; Pharmacology; Physiological; Population; Preparation; Process; Production; Proteins; Publications; Research; Research Personnel; Research Support; Resolution; Resources; Role; Sampling; Scanning Probe Microscopy; Science; Sectioning technique; Services; Signal Transduction; Specialist; Speed; System; Talents; Techniques; Testing; Three-Dimensional Imaging; Tissues; Training; Universities; Use of New Techniques; Visualization; Visualization software; West Virginia; cellular imaging; design; expectation; experimental study; fluorescence imaging; fluorescence microscope; imaging capabilities; imaging system; instrumentation; live cell imaging; malformation; optical imaging; protein transport; response; single molecule; skeletal; tool

The Marshall University Imaging Core will support the imaging activities of five junior investigators and potential pilot investigators as part of the project titled "Appalachian Center for Cellular transport in Obesity Related Disorders (ACCORD)". The five junior investigators have identified abnormalities in cellular transport related to disease states which are particularly associated with obesity, including cancer, hypertension and skeletal malformations. With the long-range goal of minimizing the health impact of dysregulated transport, or even blocking or reversing processes related to these transport mechanisms, these researchers seek to develop molecular level descriptions, which will lead to an understanding of the transport mechanism and the mechanism by which external factors can affect transport. The Imaging Core will support the long-range goals of these investigators by providing visualization tools and techniques which will enable them to determine the relative numbers and distributions of the specific proteins they have determined are associated with transport anomalies in selected disease states. The principle imaging tool which will be applied for the visualization tasks is a Multiphoton Fluorescence Microscope, which will enable high resolution optical imaging of tagged proteins. The multiphoton capability increases the quality of optical imaging in tissue, and will be used in studies comparing normal and abnormal tissues. This imaging capability will be enhanced, for researchers developing suitable model systems, via the production of custom tagged proteins suitable for use in live cell imaging. Live cell imaging will provide the opportunity to observe changes in protein concentrations and/or distributions within a cell occurring in response to a physiologically relevant cue or resulting from introduction of a pharmacological agent. Techniques enabled by fluorescent protein tagging, including FRET (fluorescence resonant energy transfer) and FRAP (fluorescence recovery after photobleaching) may be employed to test developing hypotheses of protein transport mechanisms and their anomalies. Additional capabilities, including single molecule fluorescence imaging and atomic force microscopy will be available for addressing evolving project requirements.

马歇尔大学成像中心将支持五名初级研究人员的成像活动， 潜在的试点研究人员作为项目的一部分，名为"阿巴拉契亚中心的细胞运输， 肥胖相关疾病（ACCORD）。五名初级调查员发现了 与疾病状态相关的细胞转运，特别是与肥胖相关的疾病状态，包括 癌症、高血压和骨骼畸形。长期目标是最大限度地减少 运输失调的影响，甚至阻碍或逆转与这些运输有关的进程 机制，这些研究人员寻求发展分子水平的描述，这将导致一个 了解传输机制和外部因素影响的机制 运输成像核心将通过提供以下服务来支持这些研究人员的长期目标： 可视化工具和技术，使他们能够确定相对数量， 他们确定的特定蛋白质的分布与运输异常有关， 选择疾病状态。将用于可视化任务的主要成像工具是 多光子荧光显微镜，这将使高分辨率光学成像的标记 proteins.多光子能力提高了组织中光学成像的质量，并将用于 研究比较正常和异常组织。这种成像能力将得到加强，因为 研究人员开发合适的模型系统，通过生产定制的标记蛋白质， 用于活细胞成像。活细胞成像将提供观察蛋白质变化的机会， - 响应于生理学相关线索而发生的细胞内的浓度和/或分布，或 由于引入药剂而产生的。荧光蛋白技术 标记，包括FRET（荧光共振能量转移）和FRAP（荧光恢复后， 光漂白）可用于测试蛋白质转运机制的发展假设， 他们的异常。其他功能，包括单分子荧光成像和原子力 显微镜将可满足不断变化的项目要求。