Penn TMC: Organ-Specific Project
Penn TMC:特定器官项目
基本信息
- 批准号:10461164
- 负责人:
- 金额:$ 92.42万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-24 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAdultAnatomyAtlasesBiological AssayCardiovascular DiseasesCellsCellular biologyChromatinChromiumClinicalCollaborationsCommunitiesComputer softwareDataData AnalysesData CollectionDepositionEndocrineEndocrine System DiseasesEndometriumEnsureEnvironmentEpigenetic ProcessFemaleFertilizationFetusGenerationsGeographyGerm CellsGoalsGonadal Steroid HormonesHealthHomeHumanHuman BioMolecular Atlas ProgramHuman bodyImageIn SituInformaticsLifeLocationLongevityMaintenanceMammalian OviductsMapsMedical RecordsMetadataMethodsModalityMolecularOocytesOrganOvaryPeriodicityPersonal SatisfactionPopulationProceduresProcessProductionProteinsProtocols documentationRNARecordsReproductionReproductive BiologyResourcesSamplingSchemeStromal CellsStructureTimeTissue SampleTissuesUterusValidationWalkingWomen&aposs HealthWorkannotation systembody systemcell communitydata standardsdigital modelsembryo/fetusfemale reproductive systemgranulosa cellimplantationindexingmolecular scalemortalitynatural Blastocyst Implantationreproductivereproductive organsample collectionstructured datatheca cellthree-dimensional modeling
项目摘要
The female reproductive system—comprised of the uterus, fallopian tubes and the ovaries—undergoes
some of the most dynamic changes in structure and function of any adult human organ system on a
monthly basis and throughout life. Tissue organization in the female reproductive system is paramount
as it is responsible for the coordinated cyclic changes necessary not only for reproduction but also for the
maintenance of multiple other organ systems. Here, we propose to couple our unique access to the
entire normal female reproductive system with our expertise in single cell biology to establish a multi-
scale molecular map of the female reproductive system. We will collect and manage subject/donor
samples to generate a multi-scale molecular map: We will leverage our subject/donor population to
comprehensively sample up to 22 tissue locations from the same subject (16 donors), as well as sample
a single tissue over a large number of donors to collect temporal information. In total, we propose to
collect ~700 tissue samples over 22 locations from four female reproductive organs using multiple assay
modalities. We will generate a spatially coordinated multi-scale molecular map: We will collect scRNAseq
(3 different schemes), multiplex FISH (clampFISH), in situ open chromatin, as well as simultaneous
RNA/open chromatin assays and protein assays in collaboration with other TMCs for a total of six
different assay methods. We will also develop a 3D digital model of the female reproductive system using
subject/donor imaging data, and develop associated spatial annotation tools to index our clinical sample
collection in SA1 with anatomical coordinates. All assay data will be registered to our 3D anatomical map
that will be integrated with the HIVE Common Coordinate Framework. All metadata from subject records,
clinical procedures, molecular procedures, and informatics pipelines will be collected, curated, and
deposited as structured data. We will use well-validated protocols (e.g., 10X Chromium platform) or
validate and optimize molecular assays before moving them to production pipelines. We will work with
other TMCs as well as other HuBMAP groups to develop community standards and validation methods
for the molecular assays. For systematic data collection and production pipeline work, we will construct a
streamlined process from Clinical Sampling, to 3D Model Indexing, to Molecular Assays, to Data Analysis
and Coordination. The completion of our data collection activities will create an unprecedented public
resource that will enhance our understanding of human reproductive biology and impact women’s health.
女性生殖系统--包括子宫、输卵管和卵巢--经历了
任何成人器官系统的结构和功能的一些最动态的变化,
每月一次,终生。女性生殖系统中的组织结构至关重要
因为它负责协调的周期性变化,这不仅是繁殖所必需的,
维持多个其他器官系统。在这里,我们建议将我们独特的访问权限与
利用我们在单细胞生物学方面的专业知识,
女性生殖系统的分子图。我们将收集和管理受试者/供体
样本生成多尺度分子图谱:我们将利用我们的受试者/供体人群,
从同一受试者(16名供体)全面采集多达22个组织位置,以及样本
一个单一的组织在大量的捐助者收集时间信息。总的来说,我们建议
使用多项检测从4个雌性生殖器官的22个位置采集约700份组织样本
方式。我们将生成空间协调的多尺度分子图谱:我们将收集scRNAseq
(3种不同的方案),多重FISH(clampFISH),原位开放染色质,以及同时
RNA/开放染色质分析和蛋白质分析,与其他TMC合作,共进行6次
不同的分析方法。我们还将开发女性生殖系统的3D数字模型,
受试者/供体成像数据,并开发相关的空间注释工具来索引我们的临床样本
在SA 1中收集解剖坐标。所有分析数据将配准到我们的3D解剖图
这将与HIVE共同协调框架相结合。所有目标记录的元数据,
临床程序,分子程序和信息学管道将被收集,策划,
存储为结构化数据。我们将使用经过充分验证的协议(例如,10 X Chromium平台)或
在将分子分析转移到生产管道之前验证和优化它们。我们将与
其他TMCs以及其他HuBMAP小组制定社区标准和验证方法
用于分子检测为了进行系统的数据收集和生产管道工作,我们将建立一个
从临床采样到3D模型索引、到分子测定、到数据分析的简化流程
和协调我们的数据收集活动的完成将创造一个前所未有的公众
这将提高我们对人类生殖生物学的理解,并影响妇女的健康。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Kurt T Barnhart其他文献
Kurt T Barnhart的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Kurt T Barnhart', 18)}}的其他基金
Early Double Low-Dose Aspirin to Reduce Preeclampsia and Miscarriage: a Global Approach RCT
早期双倍低剂量阿司匹林减少先兆子痫和流产:全球方法随机对照试验
- 批准号:
10711793 - 财政年份:2023
- 资助金额:
$ 92.42万 - 项目类别:
Clinical utility of novel biomarkers for prediction of early pregnancy failure
新型生物标志物预测早期妊娠失败的临床应用
- 批准号:
10563608 - 财政年份:2023
- 资助金额:
$ 92.42万 - 项目类别:
CCTN - CONTRACEPTIVE CLINICAL TRIALS NETWORK - FEMALE SITES
CCTN - 避孕临床试验网络 - 女性站点
- 批准号:
8933128 - 财政年份:2014
- 资助金额:
$ 92.42万 - 项目类别:
Development of a serum biosignature for ectopic pregnancy.
异位妊娠血清生物特征的开发。
- 批准号:
9268010 - 财政年份:2014
- 资助金额:
$ 92.42万 - 项目类别:
Development of a serum biosignature for ectopic pregnancy.
异位妊娠血清生物特征的开发。
- 批准号:
8846129 - 财政年份:2014
- 资助金额:
$ 92.42万 - 项目类别:
Development of a serum biosignature for ectopic pregnancy.
异位妊娠血清生物特征的开发。
- 批准号:
8631014 - 财政年份:2014
- 资助金额:
$ 92.42万 - 项目类别:
CCTN - CONTRACEPTIVE CLINICAL TRIALS NETWORK - FEMALE SITES
CCTN - 避孕临床试验网络 - 女性站点
- 批准号:
10329724 - 财政年份:2014
- 资助金额:
$ 92.42万 - 项目类别:
Development of a serum biosignature for ectopic pregnancy.
异位妊娠血清生物特征的开发。
- 批准号:
9473793 - 财政年份:2014
- 资助金额:
$ 92.42万 - 项目类别:
相似海外基金
Co-designing a lifestyle, stop-vaping intervention for ex-smoking, adult vapers (CLOVER study)
为戒烟的成年电子烟使用者共同设计生活方式、戒烟干预措施(CLOVER 研究)
- 批准号:
MR/Z503605/1 - 财政年份:2024
- 资助金额:
$ 92.42万 - 项目类别:
Research Grant
Early Life Antecedents Predicting Adult Daily Affective Reactivity to Stress
早期生活经历预测成人对压力的日常情感反应
- 批准号:
2336167 - 财政年份:2024
- 资助金额:
$ 92.42万 - 项目类别:
Standard Grant
RAPID: Affective Mechanisms of Adjustment in Diverse Emerging Adult Student Communities Before, During, and Beyond the COVID-19 Pandemic
RAPID:COVID-19 大流行之前、期间和之后不同新兴成人学生社区的情感调整机制
- 批准号:
2402691 - 财政年份:2024
- 资助金额:
$ 92.42万 - 项目类别:
Standard Grant
Elucidation of Adult Newt Cells Regulating the ZRS enhancer during Limb Regeneration
阐明成体蝾螈细胞在肢体再生过程中调节 ZRS 增强子
- 批准号:
24K12150 - 财政年份:2024
- 资助金额:
$ 92.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Migrant Youth and the Sociolegal Construction of Child and Adult Categories
流动青年与儿童和成人类别的社会法律建构
- 批准号:
2341428 - 财政年份:2024
- 资助金额:
$ 92.42万 - 项目类别:
Standard Grant
Understanding how platelets mediate new neuron formation in the adult brain
了解血小板如何介导成人大脑中新神经元的形成
- 批准号:
DE240100561 - 财政年份:2024
- 资助金额:
$ 92.42万 - 项目类别:
Discovery Early Career Researcher Award
Laboratory testing and development of a new adult ankle splint
新型成人踝关节夹板的实验室测试和开发
- 批准号:
10065645 - 财政年份:2023
- 资助金额:
$ 92.42万 - 项目类别:
Collaborative R&D
Usefulness of a question prompt sheet for onco-fertility in adolescent and young adult patients under 25 years old.
问题提示表对于 25 岁以下青少年和年轻成年患者的肿瘤生育力的有用性。
- 批准号:
23K09542 - 财政年份:2023
- 资助金额:
$ 92.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identification of new specific molecules associated with right ventricular dysfunction in adult patients with congenital heart disease
鉴定与成年先天性心脏病患者右心室功能障碍相关的新特异性分子
- 批准号:
23K07552 - 财政年份:2023
- 资助金额:
$ 92.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Issue identifications and model developments in transitional care for patients with adult congenital heart disease.
成人先天性心脏病患者过渡护理的问题识别和模型开发。
- 批准号:
23K07559 - 财政年份:2023
- 资助金额:
$ 92.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)