TRD4: Design and Engineering of MicroED hardware for nanocrystallization and time resolved studies

TRD 4.用于纳米晶化和时间分辨研究的MicroED硬件的设计和工程 Spence（负责人） 总结 介绍了微晶电子衍射（MicroED）的最初发展，以及随后对MicroED的改进。 新的数据收集方法和控制显微镜的硬件驱动了这项技术。的 特别重要的是将MicroED转变为具有时间分辨能力的高通量方法， 快速确定功能大分子复合物的时间依赖性结构-功能关系。这 将推动技术向前发展，但它需要额外的设计和工程努力，以使这些 想要的功能成为现实。在这里，我们将重点介绍三个MicroED工程目标：（1）开发高性能的 通过在线结晶设备提高MicroED实验的效率和成功率， (2)调整和设计新的设备和方法，进行时间分辨的MicroED实验， (3)多功能网格表面的设计，或“网格实验室”，用于小分子的流线型制备 用于MicroED结构测定的样品。该项目的目标是1。开发高通量 用于MicroED的在线结晶; 2.时间分辨微电子衍射的新样品制备方法; 3.“实验室 用于小分子的MicroED分析。总的来说，我们将设计、设计和实施新工具 和技术的三个主要方面的MicroED。这些新工具将大大加快吞吐量 提高MicroED的功能，并提高收集数据的质量。此外，新的实验模式 将通过目标2的工作成为可能，为MicroED的精确时间分辨研究打开大门。

TRD4. Design and Engineering of MicroED hardware for nanocrystallization and time resolved studies – Spence (Lead) Summary The initial development of microcrystal electron diffraction (MicroED), and subsequent improvements to the technique, have been driven by new data collection methods and hardware for controlling the microscope. Of particular importance is turning MicroED into a high-throughput method with time-resolved capability to be able to rapidly determine time-dependent structure-function relation of functioning macomolecular complexes. This will propel the technique forward, but it requires additional design and engineering efforts in order to make these desired features a reality. Here, we will focus on three MicroED engineering aims: (1) the development of high throughput on-grid crystallization equipment to increase the efficiency and success rate of MicroED experiments, (2) the adaptation and design of new equipment and methods to conduct time-resolved MicroED experiments, (3) the design of a multi-functional grid surface, or “lab on a grid” for the streamlined preparation of small molecule samples for MicroED structure determination. The aims of this project are 1. Development of high throughput on-grid crystallization for MicroED; 2. New sample preparation methodology for time-resolved MicroED; 3. “Lab on a grid” for MicroED analysis of small molecules. Overall, we will design, engineer, and implement new tools and techniques for three major aspects of MicroED. These new tools will greatly accelerate the throughput capabilities of the MicroED and improve the quality of collected data. Additionally, new experimental modalities will be made possible through the work in Aim 2, opening the door to precise time-resolved studies by MicroED.