权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Exploring knowledge gaps in childhood caries through integrated biological and clinical research

通过综合生物学和临床研究探索儿童龋齿的知识差距

基本信息

批准号：
10460965
负责人：
Nini Chaichanasakul Tran
金额：
$ 14.04万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10460965
关键词：
8 year old Address Affect Area Award Bioinformatics Biological Markers Biometry Characteristics Child Childhood Chronic Clinical Clinical Research Code Communicable Diseases Communities Comparative Genomic Analysis Course Content Dental caries Dentists Development Development Plans Diagnostic Diagnostic tests Disease Educational workshop Elements Genetic Genetic Markers Genetic Variation Genotype Goals Healthcare Heterogeneity High Prevalence Indigenous Individual Individuality Integration Host Factors Interdisciplinary Study Knowledge Lead Medical Mentors Microbiology National Institute of Dental and Craniofacial Research Oral Oral Microbiology Oral health Pediatric Dentistry Phenotype Phylogenetic Analysis Population Positioning Attribute Predisposition Preschool Child Prevalence Quality of life Research Research Design Research Personnel Resistance Saliva Salivary School Dentistry Scientist Shapes Strategic Planning Streptococcus mutans Technology Testing Therapeutic Therapeutic Intervention Time Training United States Validation Virulence Virulence Factors Vulnerable Populations base biological research career career development cariogenic bacteria clinical practice cohort diagnostic tool diagnostic value differential expression dysbiosis experimental study genome sequencing innovation insight microbial microbial community microbial host microbiome microbiome research microorganism interaction multidisciplinary next generation sequencing novel oral microbiome pre-clinical professor research and development saliva diagnostic skills stem tool transcriptome transcriptome sequencing whole genome

项目摘要

Project Summary/Abstract Dental caries, the most common, chronic, infectious childhood disease, has a substantial impact on children’s quality of life. The high prevalence of dental caries that persists despite its preventability necessitates improvement in diagnostics and therapeutic interventions for this young and vulnerable population. Over the years, significant progress has been made in caries research. Despite these advances, research gaps remain, limiting the ability to answer two fundamental questions: 1) Why do not all individuals with elevated levels of Streptococcus mutans (Sm), an important infectious, cariogenic bacterium, develop caries? 2) How does host genetic variation contribute to the susceptibility to dental caries? The former continues to be a major enigma in the field of oral microbiology. Though host susceptibility has been recognized as an important determinant in medical infectious diseases, it remains an under-studied factor in caries research. Dr. Nini Tran, an Assistant Professor at the UCLA School of Dentistry, is a pediatric dentist-scientist with a well-rounded training and a long-standing commitment to a research career. The career development plan enables Dr. Tran to gain additional expertise in three areas: 1) Microbiology and microbiome research, 2) biostatistics and bioinformatics, 3) saliva diagnostics. Dr. Tran will be supported by an outstanding multidisciplinary mentoring team with expertise to cover all elements of her research and career development. Dr. David Wong, a pioneer in the field of saliva diagnostics, is her primary mentor; Dr. Wenyuan Shi, Dr. Jeff Miller, Dr. Renate Lux, Dr. Xuesong He, Dr. Floyd Dewhirst, and Dr. Ann Griffen are the leading microbiology and microbiome experts; Dr. David Elashoff provides biostatistics support, while Dr. Jeff McLean has expertise in next-generation sequencing and bioinformatics analyses. Through a tailored curriculum of courses, workshops and the proposed research, Dr. Tran will develop crucial skills to achieve her goal of becoming a successful independent clinician-scientist in the field of childhood caries diagnostics and therapeutics. This research investigates a discordant, preschool child population whose caries status contradicts what is expected based on their Sm level in comparison to the accordance counterpart. Integrating innovative study design and novel tools, the research plan aims to 1) characterize Sm strain individuality and its ecological relationship within different microbial communities, 2) explore salivary host biomarkers related to childhood caries, and 3) validate Sm-associated microbial virulence signatures and host salivary biomarkers for precise and comprehensive caries assessment. This research will generate the basis for R01 proposals, focusing on oral microbiome inter- species interaction, and host and oral microbial interaction related to childhood caries susceptibility, and the development of diagnostics and therapeutics for childhood caries. Finally, this award will position Dr. Tran to become a top tier pediatric dentist-scientist, and enable her to make a significant contribution to the field of pediatric dentistry.

项目摘要/摘要龋病是最常见的、慢性的、传染性的儿童疾病，对儿童的生活质量。尽管龋齿是可以预防的，但龋齿的高患病率仍然存在，这就需要改善对这一年轻和弱势群体的诊断和治疗干预措施。超过了多年来，龋病研究取得了重大进展。尽管取得了这些进展，但研究差距依然存在，限制回答两个基本问题的能力：1)为什么不是所有水平升高的人变形链球菌(Sm)，一种重要的传染性致龋性细菌，会发生龋病吗？2)宿主是如何基因变异与龋齿易感性有关吗？前者仍然是中国的一个主要谜团口腔微生物学领域。尽管寄主易感性被认为是一种重要的决定因素在医学感染性疾病中，它仍然是龋病研究中一个研究较少的因素。助理Nini Tran博士加州大学洛杉矶分校牙科学院教授，儿科牙科医生兼科学家，受过全面的培训，长期致力于研究事业。职业发展计划使陈博士能够获得三个领域的其他专门知识：1)微生物学和微生物组研究，2)生物统计和生物信息学，3)唾液诊断。Tran博士将得到杰出的多学科指导拥有专业知识的团队涵盖了她的研究和职业发展的所有要素。黄大伟博士，先驱者在唾液诊断学领域，是她的主要导师；施文元博士、杰夫·米勒博士、Renate Lux博士、Dr。何学松、弗洛伊德·德惠斯特博士和安·格里芬博士是领先的微生物学和微生物组专家； David Elashoff提供生物统计学支持，而Jeff McLean博士拥有下一代的专业知识测序和生物信息学分析。通过定制的课程、研讨会和拟议的研究，陈博士将发展关键技能，以实现她成为一个成功的独立临床医生-儿童龋齿诊断和治疗领域的科学家。这项研究调查了一个不和谐的学龄前儿童群体，他们的龋齿状况与预期的情况相矛盾与相应的对应物相比，它们的Sm水平。将创新的学习设计与新颖的设计相结合工具，本研究计划旨在1)表征Sm菌株的个性及其在不同的微生物群落，2)探索与儿童龋齿有关的唾液宿主生物标志物，3)验证 SM相关微生物毒力特征和宿主唾液生物标志物的精确和全面龋齿评估。这项研究将为R01提案奠定基础，重点是口腔微生物组之间的关系与儿童龋齿易感性相关的物种相互作用、宿主和口腔微生物相互作用，以及儿童龋病诊断与治疗的研究进展。最后，这个奖项将使Tran博士成为一名顶尖的儿科牙科医生和科学家，并使她能够在儿科牙科。