VIral and host mechanisms that tilt the HSV lytic/latent balance
导致 HSV 裂解/潜伏平衡倾斜的病毒和宿主机制
基本信息
- 批准号:10460505
- 负责人:
- 金额:$ 213万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-07-02 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:AffectAlzheimer&aposs DiseaseAnimal ExperimentsAnimalsAntibodiesAreaAttenuated VaccinesAutophagocytosisAutophagosomeBackBehavioralBiogenesisBiological AssayBiologyCCCTC-binding factorChromatinCollaborationsCultured CellsEncephalitisEnsureEquilibriumExperimental DesignsGangliaGene ExpressionGenetic TranscriptionHerpesvirus 1HeterochromatinHumanImmediate-Early GenesImmuneImmune responseImmunityIn VitroInvestigationKeratitisLaboratoriesLeadLifeLyticLytic PhaseLytic VirusMaintenanceMicroRNAsMitochondrial ProteinsMusNervous system structureNeurodegenerative DisordersNeuronsNuclear ExportPathogenesisPlayProteinsRNARegulationRepressionRoleSeriesSimplexvirusSystemTestingVaccinesViralViral GenesViral GenomeViral ProteinsVirusVirus DiseasesVirus LatencyVirus ReplicationWidespread DiseaseWorkadaptive immune responsecombatgene producthuman diseasein vitro Modelin vivolatency associated transcriptlatent infectionlytic gene expressionmutantnovel strategiespre-miRNApreventprogramsreactivation from latencyresponsestatisticsviral DNA
项目摘要
Summary/Abstract for Overall Program
The long-term objective of this program project is to determine how herpes simplex virus
1 (HSV-1) can switch between a highly active "lytic" infection that produces infectious
virus and a more silent latent infection. There are several hypotheses regarding
mechanisms that can tilt the balance in favor of lytic infection or latent infection. These
mechanisms include viral gene products that affect the chromatin status of the viral
genome, post-transcriptional mechanisms that can affect viral and host gene expression,
and immune responses and viral gene products that combat those responses. None of
these mechanisms is solely responsible for the lytic/latent balance, and each of these
mechanisms is highly likely to be connected. For example, immune responses can affect
viral gene expression, and post-transcriptional mechanisms can repress the expression
of viral gene products that affect chromatin status and combat immune responses. Thus,
an integrated approach to the lytic/latent balance is needed. In this Program Project
proposal, three senior herpesvirologists with complementary areas of expertise will
conduct a series of highly collaborative studies to investigate these mechanisms in three
intertwined projects with the aid of three cores.
Project 1 will study (including collaborative studies with Project 2) how viral latency-
associated transcripts (LATs), the viral protein ICP0, and the host protein CTCF effect a
chromatin configuration poised for reactivation during establishment and maintenance of
latency in vivo, and with Project 3, in cultured mouse neurons, and in human neurons.
Project 2 will focus on how post-transcriptional regulatory mechanisms can repress lytic
gene expression and affect chromatin status (with Project 1) and a viral gene product
that counteracts immunity (with Projects 3 and 1) and contribute to latency, and a virus
block to nuclear export of miRNAs (with Project 1) and the targets of viral miRNAs in
cultured cells including mouse and human neurons (Projects 3 and 1).
Project 3 will use an in vitro model of latency using cultured neurons from mice, including
genetically altered strains, and viral mutants (some from Projects 1 and 2) to test roles of
neuron-specific autophagosomes, viral proteins that counter immunity, and antibodies in
the nervous system (including those generated by a vaccine from Project 1) in control of
viral replication, latency, reactivation, and, gene expression.
整体方案摘要/摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DONALD M COEN其他文献
DONALD M COEN的其他文献
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{{ truncateString('DONALD M COEN', 18)}}的其他基金
Antagonizing miRNAs in a strategy to cure HSV latency
拮抗 miRNA 来治愈 HSV 潜伏期
- 批准号:
8510128 - 财政年份:2013
- 资助金额:
$ 213万 - 项目类别:
Viral And host mechanisms that tilt the HSV lytic/latent balance
导致 HSV 裂解/潜伏平衡倾斜的病毒和宿主机制
- 批准号:
8871671 - 财政年份:2013
- 资助金额:
$ 213万 - 项目类别:
Project 2 - Post-transcriptional mechanisms and the HSV lytic/latent balance
项目 2 - 转录后机制和 HSV 裂解/潜伏平衡
- 批准号:
10226131 - 财政年份:2013
- 资助金额:
$ 213万 - 项目类别:
VIral and host mechanisms that tilt the HSV lytic/latent balance
导致 HSV 裂解/潜伏平衡倾斜的病毒和宿主机制
- 批准号:
9791972 - 财政年份:2013
- 资助金额:
$ 213万 - 项目类别:
Project 2 - Post-transcriptional mechanisms and the HSV lytic/latent balance
项目 2 - 转录后机制和 HSV 裂解/潜伏平衡
- 批准号:
9791977 - 财政年份:2013
- 资助金额:
$ 213万 - 项目类别:
Viral And host mechanisms that tilt the HSV lytic/latent balance
导致 HSV 裂解/潜伏平衡倾斜的病毒和宿主机制
- 批准号:
9102872 - 财政年份:2013
- 资助金额:
$ 213万 - 项目类别:














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