Vector Immunology Core

核心A--摘要/摘要： 媒介免疫学核心将直接支持该方案项目的所有四个项目 通过提供可靠、一致和标准化的免疫学检测进行赠款。它将专注于 创建、验证和执行免疫学分析以确定B细胞和T细胞的特征 小鼠和雪貂对甲型肝炎病毒的免疫反应。它还将检测布斯免疫反应，以 衣壳和转基因产物。核心将通过确定B细胞的特征来支持所有四个项目 基于转导抑制的NAB和ELISA对衣壳结合的反应 抗体和抗转基因抗体。它将开发和标准化评估的化验方法 雪貂的B细胞反应。此外，它还将表征Elispot的T细胞反应，以及 通过双抗体夹心法和流式细胞因子珠法进行细胞因子分析。最后，它将 开发用于评估雪貂免疫反应的流式细胞仪染色方案， 目前还没有得到很好的描述。总的来说，免疫学核心将提供有价值的数据， 向该计划项目赠款中的所有四个项目提供支持和资源，这将使 更好地了解免疫系统在基因和细胞治疗中的作用。

CORE A - SUMMARY/ABSTRACT: The Vector Immunology Core will directly support all four projects of this program project grant by providing reliable, consistent and standardized immunological assays. It will focus on creating, validating and performing immunological assays to characterize B-cell and T-cell immune responses to AAV, in mice and ferrets. It will also assay booth immune responses to capsid and to transgene product. The core will support all four projects by characterizing B-cell responses by transduction inhibition based NAB assays and ELISAs for capsid binding antibodies and anti-transgene antibodies. It will develop and standardize assays for evaluating ferret B-cell responses. It will additionally characterize T-cell responses by ELISPOT, and cytokine profiling through both ELISA and flow-based cytokine bead assays. Finally, it will develop flow cytometry staining protocols for the evaluation of Ferret immune responses, which are currently not well characterized. Overall the immunology core will provide valuable data, support and resources to all four projects within this program project grant, which will allow for better understanding of the role the immune system in gene and cell therapies.