Vector Immunology Core

载体免疫学核心

• 批准号: 10463804
• 负责人: Guangping Gao
• 金额: $ 33.5万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-09 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10463804
• 关键词: Antibodies; Antigens; B-Lymphocytes; Binding; Biological Assay; Capsid; Cell Therapy; Cells; Clinic; Clinical Trials; Clustered Regularly Interspaced Short Palindromic Repeats; Cystic Fibrosis; Data; Disease; Disease model; Dose; Engineering; Enzyme-Linked Immunosorbent Assay; Evaluation; Ferrets; Flow Cytometry; Gene Delivery; Gene Transduction Agent; Goals; Grant; Human; Humoral Immunities; Immune; Immune response; Immune system; Immunity; Immunological Models; Immunology; Immunology procedure; Measures; Mediating; Modeling; Monkeys; Mus; Neutralizing antibody assay; Outcome; Patients; Program Research Project Grants; Proteins; Protocols documentation; Reagent; Regulatory T-Lymphocyte; Resources; Respiratory Tract Infections; Role; Serious Adverse Event; Serology; Stains; Standardization; Steroids; T cell response; T-Lymphocyte; Testing; Therapeutic; Transgenes; Virus; adeno-associated viral vector; alpha 1-Antitrypsin Deficiency; base; cell mediated immune response; clinically relevant; cytokine; cytotoxic; disease phenotype; enzyme linked immunospot assay; gene therapy; immunogenic; mouse model; novel; response; therapeutic gene; transgene expression; vector

CORE A - SUMMARY/ABSTRACT: The Vector Immunology Core will directly support all four projects of this program project grant by providing reliable, consistent and standardized immunological assays. It will focus on creating, validating and performing immunological assays to characterize B-cell and T-cell immune responses to AAV, in mice and ferrets. It will also assay booth immune responses to capsid and to transgene product. The core will support all four projects by characterizing B-cell responses by transduction inhibition based NAB assays and ELISAs for capsid binding antibodies and anti-transgene antibodies. It will develop and standardize assays for evaluating ferret B-cell responses. It will additionally characterize T-cell responses by ELISPOT, and cytokine profiling through both ELISA and flow-based cytokine bead assays. Finally, it will develop flow cytometry staining protocols for the evaluation of Ferret immune responses, which are currently not well characterized. Overall the immunology core will provide valuable data, support and resources to all four projects within this program project grant, which will allow for better understanding of the role the immune system in gene and cell therapies.

核心A -总结/摘要： Vector Immunology Core将直接支持该计划项目的所有四个项目 通过提供可靠的、一致的和标准化的免疫学检测来获得资助。将重点 创建、验证和执行免疫学测定，以表征B细胞和T细胞 在小鼠和雪貂中对AAV的免疫应答。它还将检测布斯的免疫反应， 衣壳和转基因产物。核心将通过表征B细胞来支持所有四个项目 通过基于转导抑制的NAB测定和ELISA测定衣壳结合的应答 抗体和抗转基因抗体。它将开发和标准化检测方法， 雪貂B细胞反应它还将通过ELISPOT表征T细胞应答， 通过ELISA和基于流动的细胞因子珠测定进行细胞因子谱分析。最后将 开发用于评估雪貂免疫应答的流式细胞术染色方案， 目前还没有得到很好的表征。总的来说，免疫学核心将提供有价值的数据， 支持和资源的所有四个项目在这个计划项目赠款，这将允许 更好地理解免疫系统在基因和细胞治疗中的作用。