DEVELOPMENT OF APTAMERS TO RECOGNIZE NOD-LIKE RECEPTORS IN ZEBRAFISH

本项目的目标是开发六种斑马鱼节点样受体(NLR)的适体检测试剂，这些NLR识别先天免疫细胞中的PAMP和DAMP。由于适体的结构多样性和易于合成，适体作为一种高亲和力的试剂被认为是一种有吸引力的抗体特异性结合和检测蛋白质的试剂。该项目的成功将产生适体，用于检测重要的NLR靶标，以检查斑马鱼模型的免疫反应，用于免疫学和传染病研究。

The goal of this project is to develop aptameric detection reagents for six zebrafish NOD-like receptors (NLRs) that recognize PAMPs & DAMPs in innate immune cells. Aptamers are an attractive alternative to antibodies as a high affinity reagent for specific binding and detection of proteins due to their structural diversity and ease of synthesis. The success of this project will yield aptamers for detecting important NLR targets to examine immune responses in the zebrafish model for immunology and infectious disease studies.