High-speed single molecule detection combining short DNA probes and barcoding bioinformatics for use as a new broad spectrum point-of-care diagnostic

高速单分子检测结合短 DNA 探针和条形码生物信息学，用作新的广谱护理点诊断

• 批准号: 100843
• 金额: $ 78.17万
• 依托单位: BASE4 INNOVATION LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=100843
• 关键词: speed; single; molecule; detection; combining

Based in Cambridge, Base4 is developing a single-molecule detector with the capacity for detection of up to 1 billion single molecules per second, thousands of times faster than is otherwise possible. Entirely solid-state, there is a well-worn path towards large scale production and the cost of consumables will be very low. Not reliant on enzymes or pathogen binding sites, there is significant capacity to rapidly increase the number of target pathogens which can be tested simultaneously. The aim of this project is to produce a rapid, highly sensitive, low-cost multiple analyte diagnostic for use in point-of-care specifically targeting the pathogens C. difficile, chlamydia, gonorrhea, and MRSA. The project will last 3 years and will result in pilot trials beginning in month 24. The consortium will be led by Base4, with partners including the Pathogen Sequencing Unit at the Sanger Wellcome Trust, the University of Cambridge Veterinary School, and the Pathology Dept at Addenbrookes Hospital.

Base4 总部位于剑桥，正在开发一种单分子检测器，每秒可检测多达 10 亿个单分子，速度比其他检测器快数千倍。全固态，有一条通向大规模生产的老路，而且消耗品的成本将非常低。不依赖酶或病原体结合位点，具有快速增加可同时测试的目标病原体数量的显着能力。该项目的目的是生产一种快速、高灵敏度、低成本的多种分析物诊断剂，用于专门针对艰难梭菌、衣原体、淋病和 MRSA 病原体的护理点。该项目将持续 3 年，并将于 24 个月开始进行试点试验。该联盟将由 Base4 领导，合作伙伴包括 Sanger Wellcome Trust 的病原体测序部门、剑桥大学兽医学院和 Addenbrookes 医院病理学部。