Regulation of dendritic cell function and tumor immunity by TIM-3

项目摘要 TIM-3对树突状细胞功能和肿瘤免疫的调节作用 肿瘤免疫是基于抗原特异性细胞毒性T细胞的从头激活和扩增。 淋巴细胞然而，为了影响肿瘤生长，这些T细胞还必须浸润到肿瘤中，克服肿瘤细胞的增殖能力。 抑制性环境，并避免在持久性抗原存在下变得疲惫， 被认为是免疫治疗的主要障碍。传统的树突状细胞被很好地确立为 获得性免疫反应的主要诱导物，但新出现的证据表明，它们也可能在 支持外周组织（包括肿瘤）内的T细胞活性。为了支持这一点，我们发现TIM- 3（含T细胞免疫球蛋白和粘蛋白结构域-3）由肿瘤树突细胞高度表达，并且 TIM-3阻断剂在体外和体内诱导趋化因子CXCL 9的表达，从而促进T细胞增殖。 乳腺癌模型中的细胞毒性效应子功能。在这里，我们建议识别树突状细胞 通过TIM-3改变的活化途径，确定非迁移性树突状细胞是否维持T细胞功能， 肿瘤，并确定树突状细胞表达CXCL 9在肿瘤免疫中的作用。这些研究将 描绘一个假定的树突状细胞调节途径，提高我们对树突状细胞的作用的理解。 肿瘤内的细胞，这两个因素，可能有重要意义的设计组合 免疫疗法

PROJECT SUMMARY Regulation of dendritic cell function and tumor immunity by TIM-3 Tumor immunity is predicated upon the de novo activation and expansion of antigen-specific cytotoxic T lymphocytes. However, to impact tumor growth these T cells must also infiltrate into tumors, overcome a suppressive environment, and avoid becoming exhausted in the presence of persistent antigen, barriers that are thought to be major impediments to immunotherapy. Conventional dendritic cells are well established as the central inducers of the adaptive immune response, but emerging evidence suggests they may also play in supporting T cell activity within peripheral tissues, including tumors. In support of this, we have found that TIM- 3 (T-cell immunoglobulin and mucin domain containing-3) is highly expressed by tumor dendritic cells, and that TIM-3 blockade induces expression of the chemokine CXCL9 in vitro and in vivo, thereby promoting T cell cytotoxic effector function in models of mammary carcinoma. Here we propose to identify the dendritic cell activation pathways altered by TIM-3, determine if non-migratory dendritic cells maintain T cell function within tumors, and determine the role of CXCL9 expression by dendritic cells in tumor immunity. These studies will delineate a putative dendritic cell regulatory pathway and improve our understanding of the role of dendritic cells within tumors, both factors that may have important implications for the design of combinatorial immunotherapies.