Training in isotopic techniques for metabolic research

项目摘要 此申请是对融资机会公告的回应，PAR-15-139，为期五天 《代谢研究中的同位素示踪剂：动力学原理与实践》课程 分析“。本课程讲师将介绍最先进的使用放射性和 用于研究体内全身和器官代谢及细胞内通量的稳定同位素 体内和体外的速率和途径调节。建模的基本方面是 考虑以及在碳水化合物、脂肪、蛋白质代谢研究中的具体应用 和能量平衡。关于样品质量分析的理论和实践问题 将讨论光谱分析和核磁共振，包括详细的计算数值例子 参与确定同位素浓缩和基本动力学参数。高级讲座 将更详细地讨论细胞内位置和质量同位素分析的使用 通量速率和蛋白质和氨基酸代谢的各个方面。具体应用 (高胰岛素正常血糖钳)，用于人类和动物。这个 课程采用课堂讲授与问题讨论相结合的形式。此外，还有一个流行的方面 当然，实习生与课程教员一对一地会面，讨论他们的具体要求 研究项目，并可以在晚间会议上介绍他们的项目。一项新的增加是详细的 以网络研讨会的形式监控特定路径的理论和实验协议 将在课程网站上向整个研究社区开放。通常为75-85 每年都有学员参加该课程。与会者的反馈非常好评。一个 一些受训人员利用同位素技术开发了新的研究项目。因此， 这门受欢迎的课程建立在学习新陈代谢所需的基本技能的基础上，并加强了这些技能。 与NIDDK任务相关的进程。

Project Summary This application is in response to Funding Opportunity Announcement PAR-15-139 a five day course entitled” Isotope Tracers in Metabolic Research: Principles and Practice in Kinetic Analysis”. The course faculty will present state of the art methods for using both radioactive and stable isotopes to investigate whole body and organ metabolism in vivo and intracellular flux rates and pathway regulation in vivo and in vitro. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Specific applications (hyperinsulinemic euglycemic clamp) for use in humans and animals will be presented. The course uses a lecture format combined with problem discussions. In addition a popular aspect of the course is that trainees meet one on one with course faculty to discuss their specific research project and can present their project in an evening session. A new addition is detailed theory and experimental protocols to monitor specific pathways in a webinar style format that will be available on the course website to the entire research community. Typically, 75-85 trainees attended the course each year. Feedback from attendees has been very laudatory. A number of trainees have developed new research projects using isotope technologies. Thus this popular course builds on and reinforces fundamental skills needed to study metabolic processes that are relevant to the mission of NIDDK.