Training in isotopic techniques for metabolic research

项目摘要 此申请是对融资机会公告PAR-15-139的回应， 代谢研究中的同位素示踪剂：动力学原理与实践 分析”。该课程的教师将目前的最先进的方法，使用放射性和 研究体内全身和器官代谢以及细胞内通量的稳定同位素 率和途径调节在体内和体外。建模的基本方面包括 被认为是，以及具体的应用研究碳水化合物，脂肪，蛋白质代谢 和能量平衡。与样品质量分析有关的理论和实践问题 光谱和核磁共振将讨论，包括详细的计算数值的例子 参与确定同位素富集和基本动力学参数。高级讲座 将更详细地讨论使用位置和质量同位素分析细胞内 通量率和蛋白质和氨基酸代谢的各个方面。特定应用 （高胰岛素正葡萄糖钳夹）用于人类和动物。的 本课程采用讲课与问题讨论相结合的形式。此外，一个受欢迎的方面 学员与课程教员一对一会面，讨论他们的具体问题， 研究项目，并可以在晚上的会议上介绍他们的项目。一个新的补充是详细的 理论和实验协议，以网络研讨会风格的格式监测特定的途径， 将在课程网站上提供给整个研究界。通常，75-85 学员每年都参加培训班。与会者的反馈非常令人称赞。一 一些受训人员利用同位素技术开发了新的研究项目。因此 这门受欢迎的课程建立在并加强了研究代谢所需的基本技能 与NIDDK的使命相关的过程。

Project Summary This application is in response to Funding Opportunity Announcement PAR-15-139 a five day course entitled” Isotope Tracers in Metabolic Research: Principles and Practice in Kinetic Analysis”. The course faculty will present state of the art methods for using both radioactive and stable isotopes to investigate whole body and organ metabolism in vivo and intracellular flux rates and pathway regulation in vivo and in vitro. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Specific applications (hyperinsulinemic euglycemic clamp) for use in humans and animals will be presented. The course uses a lecture format combined with problem discussions. In addition a popular aspect of the course is that trainees meet one on one with course faculty to discuss their specific research project and can present their project in an evening session. A new addition is detailed theory and experimental protocols to monitor specific pathways in a webinar style format that will be available on the course website to the entire research community. Typically, 75-85 trainees attended the course each year. Feedback from attendees has been very laudatory. A number of trainees have developed new research projects using isotope technologies. Thus this popular course builds on and reinforces fundamental skills needed to study metabolic processes that are relevant to the mission of NIDDK.