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Acquisition of a Surface Plasmon Resonance Biosensor: Enhancing Biomedical (Bio)Molecular Recognition Projects

获得表面等离子共振生物传感器：增强生物医学（生物）分子识别项目

基本信息

批准号：
8826435
负责人：
Janarthanan Jayawickramarajah
金额：
$ 35.4万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-03-09 至 2016-03-08
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Tulane University has a strong commitment to conducting cutting-edge biomedical research and its investigators have received over $50 million in NIH funds in the last fiscal year alone. In particular, Tulane aims to achieve its long-term biomedical goal of alleviating the burden of disease by fostering interdisciplinary teams of researchers to work together to solve over-arching biomedical issues. As part of this effort, a group of eleven investigators spanning three Schools (Science/Engineering, Tropical Medicine/Public Health, and Medicine) at Tulane and two researchers from nearby Xavier University of Louisiana, an HBCU, are working to develop a core of excellence in (bio)molecular recognition. This diverse group of researchers, ranging from chemists to virologists to immunologists, are studying fundamental aspects of various protein-protein, protein-small molecule, protein-DNA, DNA-small molecule, and small molecule-small molecule interactions, with the ultimate goal of aiding in the development of therapeutic and diagnostic agents for diseases ranging from cancer to malaria to viral fever. What is a common thread in these projects is the critical need for quantitative characterization of kinetic and thermodynamic parameters of (bio)molecular interactions. While this core of researchers has access to many other techniques for measuring binding (including fluorescence polarization, isothermal titration calorimetry, and nuclear magnetic resonance), a key cornerstone for the analysis of (bio)molecular interactions is lacking. Specifically, Tulane does not possess a functional surface plasmon resonance (SPR) instrument. The requested SPR based Biacore T200 is especially suited for the needs of the user group because it requires minimal sample, has high sensitivity, is high throughput, and has built in software for immunological screening. The Biacore T200 will be housed in the John Bennett Johnston Health and Environmental Research Building (JBJ) building that has been newly renovated, via NIH funds, with the precise goal of fostering interdisciplinary research. The Biacore T200 will be operated and maintained by Dr. D. Grimm, an instrumentation specialist, who has over 20 years of experience working in the Tulane Central Instrumentation Facility and currently is the assistant director of the facility. The servie contract and yearly supplies for the T200 will be covered from user fees with the explicit backstop from the leadership of Tulane.

描述（由申请人提供）：杜兰大学致力于开展尖端生物医学研究，其研究人员仅在上一财政年度就获得了超过5000万美元的NIH基金。特别是，杜兰旨在通过培养跨学科研究团队来共同解决总体生物医学问题，从而实现减轻疾病负担的长期生物医学目标。作为这项工作的一部分，来自杜兰大学三个学院（科学/工程、热带医学/公共卫生和医学）的11名研究人员和来自附近的路易斯安那州泽维尔大学（HBCU）的两名研究人员正在努力开发（生物）分子识别的卓越核心。从化学家到病毒学家再到免疫学家，这一多样化的研究小组正在研究各种蛋白质-蛋白质、蛋白质-小分子、蛋白质- dna、dna -小分子以及小分子-小分子相互作用的基本方面，其最终目标是帮助开发从癌症到疟疾再到病毒性发热等疾病的治疗和诊断试剂。这些项目的共同点是对（生物）分子相互作用的动力学和热力学参数的定量表征的迫切需要。虽然这一核心研究人员可以使用许多其他测量结合的技术（包括荧光极化，等温滴定量热法和核磁共振），但缺乏分析（生物）分子相互作用的关键基石。具体来说，杜兰大学没有一个功能性表面等离子体共振（SPR）仪器。所要求的基于SPR的Biacore T200特别适合用户组的需求，因为它需要最少的样品，具有高灵敏度，高通量，并且内置了免疫筛选软件。Biacore T200将被安置在约翰·贝内特·约翰斯顿健康与环境研究大楼（JBJ）大楼内，该大楼已通过美国国立卫生研究院的资金重新装修，其确切目标是促进跨学科研究。Biacore T200将由仪器专家Dr. D. Grimm博士操作和维护，他在杜兰中央仪器设施工作了20多年，目前是该设施的助理主任。在杜兰大学领导层的明确支持下，T200的服务合同和年度供应将从用户费用中支付。