Cutaneous Phenomics and Transcriptomics

皮肤表型组学和转录组学

• 批准号: 10477230
• 负责人: John T. Seykora
• 金额: $ 25.58万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10477230
• 关键词: Animal Model; Animals; Archives; Autopsy; Biological Assay; Biological Markers; Biology; Biopsy Specimen; Cells; Collaborations; Collection; Consultations; Coupled; Cutaneous; Dermatology; Development; Disease; Environment; Equipment; Experimental Models; Faculty; Formalin; Gene Expression; Genomic DNA; Genomics; Genotype; Goals; Grant; Heterogeneity; Histologic; Histology; Human; Image; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Infrastructure; Institution; Investigation; Knowledge; Laboratories; Lasers; Libraries; Messenger RNA; Microscopy; Mission; Molecular; Molecular Target; Mus; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Paraffin; Pennsylvania; Phenotype; Population; Production; Proteins; Protocols documentation; RNA; Reporting; Research; Research Personnel; Resources; Sampling; Scientific Advances and Accomplishments; Services; Skin; Specimen; Suspensions; Talents; Techniques; Technology; Testing; Time; Tissues; Universities; Veterinary Schools; Water; base; cost effective; detection assay; exome; exome sequencing; experience; genomic RNA; improved; in vivo; in vivo imaging; innovation; insight; laser capture microdissection; medical schools; member; next generation sequencing; novel; phenomics; service utilization; single cell sequencing; single-cell RNA sequencing; skin barrier; skin disorder; success; therapeutic target; tissue processing; transcriptome; transcriptome sequencing; transcriptomics; two-photon

PROJECT SUMMARY The Cutaneous Phenomics and Transcriptomics Core (CPAT) of the Penn Skin Biology and Diseases Resource-based Center (SBDRC) furthers the NIAMS mission by providing a broad array of valuable services enabling investigators to define the cutaneous phenotype, genotype and transcriptome of their cutaneous experimental models. CPAT provides unparalleled expertise in highly utilized services ranging from tissue processing and tissue immunohistochemistry/immunofluorescence to mRNA in situ hybridization and access to millions of archived skin samples and expert skin histology consultations. Excitingly, CPAT now offers an innovative collection of sophisticated experimental approaches for analyzing skin samples including laser- capture microdissection of skin samples coupled with RNA or whole-exome sequencing, single-cell RNA sequencing, in vivo two-photon and macrofluorescence imaging of skin, and multiple sophisticated assays to evaluate skin barrier function. Together, these synergistic services enable investigators to rigorously interrogate their experimental model and answer critical outstanding questions within skin biology and disease. CPAT has assembled a talented and experienced team to provide its members with the highest level of quality across its services. CPAT faculty and staff possess extensive expertise in providing all of these services and this experience, coupled with the outstanding environment in Penn Dermatology, will provide innovative and robust results to investigators evaluating hypotheses in cutaneous biology. Together, CPAT provides an economy of scale across its services that conserves the financial resources of users thereby furthering the research of members and will promote new collaborations between investigators in the field of cutaneous biology. In summary, CPAT services will drive new discoveries in cutaneous biology and disease and create new knowledge to identify novel disease biomarkers and therapeutic targets.

项目摘要 宾夕法尼亚州皮肤生物学和疾病的皮肤现象学和转录组学核心（CPAT） 基于资源的中心（SBDRC）通过提供广泛的宝贵服务来进一步推动NIAMS的任务 使研究人员能够定义皮肤的皮肤表型，基因型和转录组 实验模型。 CPAT在高度利用的服务中提供无与伦比的专业知识 加工和组织免疫组织化学/免疫荧光对mRNA的原位杂交并进入 数以百万计的归档皮肤样品和专家皮肤组织学咨询。令人兴奋的是，CPAT现在提供 精致实验方法的创新收集，用于分析包括激光在内的皮肤样品 捕获与RNA或全外观测序的单细胞RNA的皮肤样品的微分解 测序，体内两光子和皮肤的宏荧光成像，以及多个复杂的测定 评估皮肤屏障功能。这些协同服务共同使研究人员能够严格 询问他们的实验模型，并回答皮肤生物学和疾病中的关键问题。 CPAT组装了一支才华横溢的团队，为其成员提供最高水平的质量 跨它的服务。 CPAT教职员工在提供所有这些服务方面具有广泛的专业知识， 这种体验，再加上宾夕法尼亚皮肤病学的杰出环境，将提供创新的和 评估皮肤生物学假设的研究者的强大结果。一起，CPAT提供了 跨越其服务的规模经济，可以保存用户的财务资源，从而进一步发展 对成员的研究，并将促进皮肤领域调查人员之间的新合作 生物学。总而言之，CPAT服务将推动皮肤生物学和疾病中的新发现，并创建 确定新型疾病生物标志物和治疗靶标的新知识。