Cutaneous Phenomics and Transcriptomics

项目总结 宾夕法尼亚大学皮肤生物学和疾病的皮肤表型组学和转录组学核心(Cpat) 基于资源的中心(SBDRC)通过提供一系列有价值的服务进一步推动了NIAMS的使命 使研究人员能够确定他们皮肤的表型、基因和转录组 实验模型。CPAT在高使用率的服务领域提供无与伦比的专业知识，从组织 加工和组织免疫组织化学/免疫荧光原位杂交及获得 数以百万计的存档皮肤样本和专家皮肤组织学咨询。令人兴奋的是，Cpat现在提供了一个 用于分析皮肤样本的创新实验方法集合，包括激光- 捕获皮肤样本的显微解剖结合RNA或整个外显子组测序，单细胞RNA 测序、皮肤的体内双光子和宏观荧光成像以及多种复杂的分析方法 评估皮肤屏障功能。总之，这些协同服务使调查人员能够严格地 询问他们的实验模型，并回答皮肤生物学和疾病领域的关键悬而未决的问题。 CPAT组建了一支才华横溢、经验丰富的团队，为其成员提供最高水平的质量 它的所有服务。CPAT教职员工在提供所有这些服务方面拥有广泛的专业知识和 这一经验，再加上宾夕法尼亚大学皮肤科的出色环境，将提供创新和 给评估皮肤生物学假说的研究人员带来了稳健的结果。总之，Cpat提供了一种 整个服务的规模经济，节省用户的财务资源，从而进一步 成员的研究，并将促进皮肤领域研究人员之间的新合作 生物学。总之，CPAT服务将推动皮肤生物学和疾病的新发现，并创造 识别新的疾病生物标记物和治疗靶点的新知识。

PROJECT SUMMARY The Cutaneous Phenomics and Transcriptomics Core (CPAT) of the Penn Skin Biology and Diseases Resource-based Center (SBDRC) furthers the NIAMS mission by providing a broad array of valuable services enabling investigators to define the cutaneous phenotype, genotype and transcriptome of their cutaneous experimental models. CPAT provides unparalleled expertise in highly utilized services ranging from tissue processing and tissue immunohistochemistry/immunofluorescence to mRNA in situ hybridization and access to millions of archived skin samples and expert skin histology consultations. Excitingly, CPAT now offers an innovative collection of sophisticated experimental approaches for analyzing skin samples including laser- capture microdissection of skin samples coupled with RNA or whole-exome sequencing, single-cell RNA sequencing, in vivo two-photon and macrofluorescence imaging of skin, and multiple sophisticated assays to evaluate skin barrier function. Together, these synergistic services enable investigators to rigorously interrogate their experimental model and answer critical outstanding questions within skin biology and disease. CPAT has assembled a talented and experienced team to provide its members with the highest level of quality across its services. CPAT faculty and staff possess extensive expertise in providing all of these services and this experience, coupled with the outstanding environment in Penn Dermatology, will provide innovative and robust results to investigators evaluating hypotheses in cutaneous biology. Together, CPAT provides an economy of scale across its services that conserves the financial resources of users thereby furthering the research of members and will promote new collaborations between investigators in the field of cutaneous biology. In summary, CPAT services will drive new discoveries in cutaneous biology and disease and create new knowledge to identify novel disease biomarkers and therapeutic targets.