Cutaneous Phenomics and Transcriptomics

项目摘要 皮肤表型组学和转录组学核心（CPAT）的宾州皮肤生物学和疾病 基于资源的中心（SBDRC）通过提供广泛的有价值的服务来进一步推进NIAMS的使命 使研究人员能够确定皮肤表型，基因型和转录组的皮肤 实验模型CPAT在高利用率服务方面提供无与伦比的专业知识， 处理和组织免疫组织化学/免疫荧光mRNA原位杂交和访问 数以百万计的存档皮肤样本和专家皮肤组织学咨询。令人兴奋的是，CPAT现在提供了一个 用于分析皮肤样本的先进实验方法的创新集合，包括激光- 皮肤样本的捕获显微切割结合RNA或全外显子组测序，单细胞RNA 测序、皮肤的体内双光子和宏观荧光成像，以及多种复杂的测定， 评估皮肤屏障功能。总之，这些协同服务使调查人员能够严格地 询问他们的实验模型，并回答皮肤生物学和疾病中关键的悬而未决的问题。 CPAT已经组建了一支有才华和经验丰富的团队，为会员提供最高水平的质量 在其服务。CPAT的教职员工在提供所有这些服务方面拥有广泛的专业知识， 这种经验，再加上宾夕法尼亚皮肤科的优秀环境，将提供创新和 研究人员在评估皮肤生物学的假设，稳健的结果。CPAT共同提供了一个 在其服务中实现规模经济，节省用户的财务资源，从而促进 成员的研究，并将促进研究人员在皮肤领域的新合作 生物学总之，CPAT服务将推动皮肤生物学和疾病的新发现， 新的知识，以确定新的疾病生物标志物和治疗靶点。

PROJECT SUMMARY The Cutaneous Phenomics and Transcriptomics Core (CPAT) of the Penn Skin Biology and Diseases Resource-based Center (SBDRC) furthers the NIAMS mission by providing a broad array of valuable services enabling investigators to define the cutaneous phenotype, genotype and transcriptome of their cutaneous experimental models. CPAT provides unparalleled expertise in highly utilized services ranging from tissue processing and tissue immunohistochemistry/immunofluorescence to mRNA in situ hybridization and access to millions of archived skin samples and expert skin histology consultations. Excitingly, CPAT now offers an innovative collection of sophisticated experimental approaches for analyzing skin samples including laser- capture microdissection of skin samples coupled with RNA or whole-exome sequencing, single-cell RNA sequencing, in vivo two-photon and macrofluorescence imaging of skin, and multiple sophisticated assays to evaluate skin barrier function. Together, these synergistic services enable investigators to rigorously interrogate their experimental model and answer critical outstanding questions within skin biology and disease. CPAT has assembled a talented and experienced team to provide its members with the highest level of quality across its services. CPAT faculty and staff possess extensive expertise in providing all of these services and this experience, coupled with the outstanding environment in Penn Dermatology, will provide innovative and robust results to investigators evaluating hypotheses in cutaneous biology. Together, CPAT provides an economy of scale across its services that conserves the financial resources of users thereby furthering the research of members and will promote new collaborations between investigators in the field of cutaneous biology. In summary, CPAT services will drive new discoveries in cutaneous biology and disease and create new knowledge to identify novel disease biomarkers and therapeutic targets.