Histology and Tissue Characterization

皮肤组织和特征核心(SHCC)将为SDRC调查人员提供以下服务 帮助他们对来自动物模型的皮肤样本进行组织病理学和分子表征， 主要是突变或基因工程小鼠。此外，这个核心将提供分段和 未鉴定、福尔马林固定、石蜡包埋的皮肤病理学档案的组织病理学分析 样品由Core B提供。SCC的目标是：1)在加工中提供专家服务， 研究动物皮肤标本的包埋、切片和染色，包括小鼠胚胎， 以及对存档的、未识别的人体组织进行切片和染色；2)提供专家服务和 利用皮肤样本进行蛋白质免疫组织化学染色方面的培训；建立并提供建议 确定新抗体的有效染色方案；3)提供专家服务和培训 使用放射性同位素和比色法对皮肤样本进行RNA原位杂交；4)提供 动物和人类皮肤组织学组织病理学评估方面的专家解释和培训。我们 将确保动物模型表型与人类疾病之间的准确相关性。 小细胞肺癌将建立在皮肤科现有的实验室基础设施上 以加强宾夕法尼亚大学的皮肤生物学研究。拟议的小岛屿发展中国家将是一个 共享设施将帮助研究人员在更多的环境中进行他们独立资助的研究项目 节省时间和成本的方式。小组委员会将整合人手和提高成本效益，包括 以更低的成本提供高质量的服务，而不是每个调查员尝试建立这些服务 单独的分析技术。小城镇发展中心的服务将帮助国家发改委调查人员确定 他们的转基因小鼠模型在组织学、蛋白质和mRNA水平上的表型，以及 然后将这些数据与相关的人类疾病联系起来。这类服务对于产生声音至关重要 将动物模型与人类疾病联系起来的科学假设。小岛屿国家委员会将与B核心密切互动 协调皮肤病理学存档标本的染色和分析。确保高质量 服务，小城镇发展委员会将建立一个监督委员会。SCC还将通过以下方式与核心C进行交互 提供重组皮肤的组织学、免疫组织化学和mRNA原位杂交分析 由该核产生的角质形成细胞异种移植。这些服务将吸引老牌调查人员 皮肤生物学，并协助年轻研究人员为他们的研究项目获取重要数据。 相关性(请参阅说明)： 皮肤组织学和表征核心将通过提供 为皮肤组织提供经济高效、高质量的服务，包括：加工、染色、蛋白质 免疫组织化学、信使核糖核酸原位杂交和组织学评价。这些服务将允许 研究人员有效地将动物模型的皮肤表型与人类疾病联系起来。

The Skin Histology and Characterization Core (SHCC) will provide SDRC investigators with services to aid them in the histopathologic and molecular characterization of skin samples derived from animal models, primarily mutant or genetically engineered mice. In addition, this core will provide sectioning and histopathological analysis of de-identified, formalin fixed, paraffin embedded dermatopathology archived samples provided by Core B. The aims of the SHCC are: 1) To provide expert service in the processing, embedding, sectioning, and staining of skin specimens from research animals, including murine embryos, and sectioning and staining of archived, de-identified human tissue; 2) To provide expert service and training in protein immunohistochemical staining using skin samples; to establish and provide advice in determining effective staining protocols for novel antibodies; 3) To provide expert service and training for RNA in situ hybridization of skin samples using radioisotopic and colorimetric methodologies; 4) To provide expert interpretation and training in the histopathological evaluation of animal and human skin histology. We will ensure accurate correlations between animal model phenotypes and human diseases. The SHCC will build upon the existing laboratory infrastructure operating in the Department of Dermatology to enhance cutaneous biology research at the University of Pennsylvania. The proposed SHCC will be a shared facility that will help investigators conduct their independently NIH funded research projects in a more time- and cost-efficient manner. The SHCC will consolidate manpower and promote cost-effectiveness by providing high quality services at a lower cost than if each investigator were to attempt to establish these analytical techniques individually. The SHCC services will help SDRC investigators characterize the phenotype of their genetically engineered mouse models at the histologic, protein, and mRNA levels, and then correlate this data with relevant human diseases. Such services are critical for generating sound scientific hypotheses linking animal models with human diseases. The SHCC will interact closely with Core B in coordinating the staining and analysis of archived dermatopathology specimens. To assure high quality service, the SHCC will establish an oversight committee. The SHCC will also interact with Core C by providing histologic, immunohistochemical, and mRNA in situ hybridization analysis of reconstituted skin and keratinocyte xenografts produced by this core. These services will attract established investigators to cutaneous biology and assist young investigators in obtaining important data for their research projects. RELEVANCE (See instructions): The Skin Histology and Characterization Core will facilitate research on cutaneous diseases by providing cost-effective, high quality services for skin tissue that include the following: processing, staining, protein immunohistochemistry, mRNA in situ hybridization, and histologic evaluation. These services will allow investigators to effectively correlate the cutaneous phenotype of an animal model with a human disease.