Histology and Tissue Characterization

组织学和组织表征

• 批准号: 7678116
• 负责人: John T. Seykora
• 金额: $ 17.64万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7678116
• 关键词: Animal Experimentation; Animal Model; Animals; Antibodies; Archives; Artificial skin; Biology; Clinical; Collaborations; Core Facility; Cutaneous; Data; Dermatology; Disease; Embryo; Ensure; Equipment; Evaluation; Formalin; Funding; Genetically Engineered Mouse; Histologic; Histology; Human; Human Resources; Immunohistochemistry; In Situ Hybridization; Instruction; Laboratories; Link; Messenger RNA; Methodology; Microscopic; Modeling; Molecular; Mus; Paraffin Embedding; Pennsylvania; Phenotype; Process; Proteins; Protocols documentation; RNA; Research; Research Infrastructure; Research Personnel; Research Project Grants; Sampling; Sectioning technique; Services; Skin; Skin Tissue; Specimen; Staining method; Stains; Techniques; Time; Tissues; Training; United States National Institutes of Health; Universities; Work; Xenograft procedure; base; biological research; cost; cost effectiveness; cost efficient; experience; human disease; human tissue; keratinocyte; member; mouse model; mutant; novel; sound

The Skin Histology and Characterization Core (SHCC) will provide SDRC investigators with services to aid them in the histopathologic and molecular characterization of skin samples derived from animal models, primarily mutant or genetically engineered mice. In addition, this core will provide sectioning and histopathological analysis of de-identified, formalin fixed, paraffin embedded dermatopathology archived samples provided by Core B. The aims of the SHCC are: 1) To provide expert service in the processing, embedding, sectioning, and staining of skin specimens from research animals, including murine embryos, and sectioning and staining of archived, de-identified human tissue; 2) To provide expert service and training in protein immunohistochemical staining using skin samples; to establish and provide advice in determining effective staining protocols for novel antibodies; 3) To provide expert service and training for RNA in situ hybridization of skin samples using radioisotopic and colorimetric methodologies; 4) To provide expert interpretation and training in the histopathological evaluation of animal and human skin histology. We will ensure accurate correlations between animal model phenotypes and human diseases. The SHCC will build upon the existing laboratory infrastructure operating in the Department of Dermatology to enhance cutaneous biology research at the University of Pennsylvania. The proposed SHCC will be a shared facility that will help investigators conduct their independently NIH funded research projects in a more time- and cost-efficient manner. The SHCC will consolidate manpower and promote cost-effectiveness by providing high quality services at a lower cost than if each investigator were to attempt to establish these analytical techniques individually. The SHCC services will help SDRC investigators characterize the phenotype of their genetically engineered mouse models at the histologic, protein, and mRNA levels, and then correlate this data with relevant human diseases. Such services are critical for generating sound scientific hypotheses linking animal models with human diseases. The SHCC will interact closely with Core B in coordinating the staining and analysis of archived dermatopathology specimens. To assure high quality service, the SHCC will establish an oversight committee. The SHCC will also interact with Core C by providing histologic, immunohistochemical, and mRNA in situ hybridization analysis of reconstituted skin and keratinocyte xenografts produced by this core. These services will attract established investigators to cutaneous biology and assist young investigators in obtaining important data for their research projects. RELEVANCE (See instructions): The Skin Histology and Characterization Core will facilitate research on cutaneous diseases by providing cost-effective, high quality services for skin tissue that include the following: processing, staining, protein immunohistochemistry, mRNA in situ hybridization, and histologic evaluation. These services will allow investigators to effectively correlate the cutaneous phenotype of an animal model with a human disease.

皮肤组织学和表征核心（SHCC）将为SDRC研究者提供以下服务： 帮助他们对来自动物模型的皮肤样本进行组织病理学和分子表征， 主要是突变或基因工程小鼠。此外，该核心将提供切片和 组织病理学分析去识别，福尔马林固定，石蜡包埋皮肤病理学存档 样品由岩心B提供。SHCC的目标是：1）在处理过程中提供专家服务， 对来自研究动物的皮肤标本进行包埋、切片和染色，包括鼠胚胎， 以及对存档的、去识别的人体组织进行切片和染色; 2）提供专家服务， 使用皮肤样本进行蛋白质免疫组织化学染色的培训;建立并提供建议， 确定新型抗体的有效染色方案; 3）为以下人员提供专家服务和培训： 使用放射性同位素和比色方法对皮肤样品进行RNA原位杂交; 4）提供 在动物和人类皮肤组织学的组织病理学评价方面提供专家解释和培训。我们 将确保动物模型表型和人类疾病之间的准确相关性。 SHCC将建立在皮肤科现有的实验室基础设施上 来加强宾夕法尼亚大学的皮肤生物学研究。拟议的SHCC将是一个 共享设施，这将有助于研究人员进行他们的独立国家卫生研究院资助的研究项目，在一个更 时间和成本效益的方式。小组委员会会整合人手，提高成本效益， 以较低的成本提供高质量的服务，而不是每个研究者试图建立这些 个别分析技术。SHCC服务将帮助SDRC调查人员描述 其基因工程小鼠模型在组织学、蛋白质和mRNA水平上的表型，以及 然后将这些数据与相关的人类疾病联系起来。这些服务对于产生声音至关重要 将动物模型与人类疾病联系起来的科学假说。SHCC将与核心B密切互动 协调对存档的皮肤病理学标本的染色和分析。确保高质量 根据该计划，SHCC将成立一个监督委员会。SHCC还将通过以下方式与核心C进行互动： 提供重建皮肤的组织学、免疫组织化学和mRNA原位杂交分析， 角质细胞异种移植物产生的核心。这些服务将吸引知名调查人员， 皮肤生物学和协助年轻的研究人员获得重要的数据，为他们的研究项目。 相关性（参见说明）： 皮肤组织学和表征核心将通过提供 具有成本效益的高质量皮肤组织服务，包括以下内容：处理，染色，蛋白质 免疫组织化学、mRNA原位杂交和组织学评价。这些服务将允许 研究人员将有效地将动物模型的皮肤表型与人类疾病相关联。