Cutaneous Phenomics and Transcriptomics

皮肤表型组学和转录组学

• 批准号: 10663982
• 负责人: John T. Seykora
• 金额: $ 25.57万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10663982
• 关键词: Animal Model; Animals; Archives; Autopsy; Biological Assay; Biological Markers; Biology; Biopsy Specimen; Cells; Collaborations; Collection; Consultations; Coupled; Cutaneous; Dermatology; Development; Disease; Environment; Equipment; Experimental Models; Faculty; Formalin; Gene Expression; Genomic DNA; Genomics; Genotype; Goals; Grant; Heterogeneity; Histologic; Histology; Human; Image; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Infrastructure; Institution; Investigation; Knowledge; Laboratories; Lasers; Libraries; Messenger RNA; Microscopy; Mission; Molecular; Molecular Target; Mus; National Institute of Arthritis, and Musculoskeletal, and Skin Diseases; Paraffin; Pennsylvania; Phenotype; Population; Production; Proteins; Protocols documentation; RNA; Reporting; Research; Research Personnel; Resources; Sampling; Scientific Advances and Accomplishments; Services; Skin; Specimen; Suspensions; Talents; Techniques; Technology; Tissues; Universities; Veterinary Schools; Water; barrier to testing; cost effective; detection assay; exome sequencing; experience; genomic RNA; improved; in vivo; in vivo imaging; innovation; insight; laser capture microdissection; medical schools; member; next generation sequencing; novel; phenomics; service utilization; single cell sequencing; single-cell RNA sequencing; skin barrier; skin disorder; success; therapeutic target; tissue processing; transcriptome; transcriptome sequencing; transcriptomics; two-photon

PROJECT SUMMARY The Cutaneous Phenomics and Transcriptomics Core (CPAT) of the Penn Skin Biology and Diseases Resource-based Center (SBDRC) furthers the NIAMS mission by providing a broad array of valuable services enabling investigators to define the cutaneous phenotype, genotype and transcriptome of their cutaneous experimental models. CPAT provides unparalleled expertise in highly utilized services ranging from tissue processing and tissue immunohistochemistry/immunofluorescence to mRNA in situ hybridization and access to millions of archived skin samples and expert skin histology consultations. Excitingly, CPAT now offers an innovative collection of sophisticated experimental approaches for analyzing skin samples including laser- capture microdissection of skin samples coupled with RNA or whole-exome sequencing, single-cell RNA sequencing, in vivo two-photon and macrofluorescence imaging of skin, and multiple sophisticated assays to evaluate skin barrier function. Together, these synergistic services enable investigators to rigorously interrogate their experimental model and answer critical outstanding questions within skin biology and disease. CPAT has assembled a talented and experienced team to provide its members with the highest level of quality across its services. CPAT faculty and staff possess extensive expertise in providing all of these services and this experience, coupled with the outstanding environment in Penn Dermatology, will provide innovative and robust results to investigators evaluating hypotheses in cutaneous biology. Together, CPAT provides an economy of scale across its services that conserves the financial resources of users thereby furthering the research of members and will promote new collaborations between investigators in the field of cutaneous biology. In summary, CPAT services will drive new discoveries in cutaneous biology and disease and create new knowledge to identify novel disease biomarkers and therapeutic targets.

项目概要 宾夕法尼亚大学皮肤生物学和疾病的皮肤表型组学和转录组学核心 (CPAT) 基于资源的中心 (SBDRC) 通过提供广泛的有价值的服务来进一步推进 NIAMS 的使命 使研究人员能够定义其皮肤的皮肤表型、基因型和转录组 实验模型。 CPAT 在高度利用的服务领域提供无与伦比的专业知识，包括组织 处理和组织免疫组织化学/免疫荧光对 mRNA 原位杂交和获取 数以百万计的存档皮肤样本和专家皮肤组织学咨询。令人兴奋的是，CPAT 现在提供 用于分析皮肤样本的复杂实验方法的创新集合，包括激光 捕获皮肤样本的显微解剖并结合 RNA 或全外显子组测序、单细胞 RNA 测序、体内双光子和皮肤宏观荧光成像以及多种复杂的检测 评估皮肤屏障功能。总之，这些协同服务使调查人员能够严格 询问他们的实验模型并回答皮肤生物学和疾病中关键的悬而未决的问题。 CPAT 组建了一支才华横溢、经验丰富的团队，为其成员提供最高质量的服务 其服务范围。 CPAT 教职员工在提供所有这些服务方面拥有丰富的专业知识，并且 这种经验，再加上宾夕法尼亚大学皮肤科的出色环境，将提供创新和 为评估皮肤生物学假设的研究人员提供了可靠的结果。 CPAT 共同提供了 其服务的规模经济，节省用户的财务资源，从而进一步促进 成员的研究并将促进皮肤领域研究人员之间的新合作 生物学。总之，CPAT 服务将推动皮肤生物学和疾病领域的新发现，并创造 识别新疾病生物标志物和治疗靶点的新知识。