Untangling the Inner Workings of the Mycobacterial Cell Envelope
解开分枝杆菌细胞膜的内部运作
基本信息
- 批准号:10478137
- 负责人:
- 金额:$ 39.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-09-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAnti-Infective AgentsAntibioticsBacteriaBehaviorBiochemistryBiogenesisBiological ProcessBiologyCellsCellular StructuresCellular biologyCharacteristicsChemicalsCollaborationsComplexDevelopmentDrug ToleranceEnvironmentGenus MycobacteriumGrowthHydrophobicityInfectionInterventionKnowledgeLipidsMembraneMicrobiologyMissionMycobacterium tuberculosisNational Institute of General Medical SciencesPathogenicityPathway interactionsPharmaceutical PreparationsPhysiologyPreventionProcessPublic HealthResearchResistanceTuberculosisadvanced diseasecell envelopedisease diagnosisenv Gene Productsimprovedmycobacterialprogramstool
项目摘要
PROJECT SUMMARY
Mycobacteria are the exception to many rules for bacteria. Their cellular structure and the way they grow defy
common assumptions. Moreover, their exceptional cell biology is believed to underlie exceptional behaviors
such as tolerance to drugs. Drug tolerance is general to mycobacteria, but is especially relevant to the
eradication of pathogenic species such as Mycobacterium tuberculosis, which causes tuberculosis. The unique
characteristics of mycobacteria could offer attractive avenues for more effectively targeting them in infection.
For example, their cell envelope is a multilayer cellular structure that is one of the most complex in the bacterial
kingdom and contributes to the inherent resistance of mycobacteria to antibiotics. However, very little is known
about how the cell envelope is made during growth and division. In particular very little is known about the
assembly of the outer membrane-like barrier known as the mycomembrane, which is a defining feature of
mycobacteria due to its unusual composition and extreme hydrophobicity. The rationale of this proposal is that
uncovering such fundamentals is essential to understanding mycobacterial survival in the challenging
environments that they encounter. The objective of this research program is to uncover mechanisms of
mycomembrane biogenesis and the connections to cell elongation. The proposal addresses three questions:
Q1: How do mycobacteria export lipids to the mycomembrane?
Q2: How do mycobacteria synthesize the multiple layers of the cell envelope and achieve localized growth
from their poles?
Q3: How do we identify the machinery for essential processes within the mycobacterial cell envelope?
The PI and her team will address these questions through a combination of microbiology, biochemistry, and
chemical biology. A network of collaborations supports this program. The impact of this project is to provide (1)
opportunities for the rational targeting of cell envelope biogenesis for anti-infective therapy and (2) much-
needed tools to investigate mycobacterial cell envelope proteins.
项目摘要
分枝杆菌是许多细菌规则的例外。它们的细胞结构和生长方式
共同的假设。此外,他们特殊的细胞生物学被认为是特殊行为的基础
例如对药物的耐受性。药物耐受性对分枝杆菌是普遍的,但特别与分枝杆菌相关。
根除致病物种,如导致结核病的结核分枝杆菌。独特的
分枝杆菌的特性可以为更有效地在感染中靶向它们提供有吸引力的途径。
例如,它们的细胞被膜是一种多层细胞结构,是细菌中最复杂的结构之一。
分枝杆菌是分枝杆菌界的一个分支,它有助于分枝杆菌对抗生素的固有耐药性。然而,
细胞膜在生长和分裂过程中是如何形成的。特别是,人们对
组装的外部膜状屏障称为菌膜,这是一个定义的功能,
分枝杆菌由于其不寻常的组成和极端的疏水性。这项建议的理由是,
揭示这些基本原理对于理解分枝杆菌在挑战性环境中的生存至关重要。
他们遇到的环境。这项研究计划的目的是揭示机制,
菌膜生物发生和细胞伸长的联系。该提案涉及三个问题:
问1:分枝杆菌如何将脂质输出到菌膜?
问题2:分枝杆菌如何合成多层细胞包膜并实现局部生长
从他们的极点?
问题3:我们如何识别分枝杆菌细胞包膜内的基本过程的机制?
PI和她的团队将通过微生物学,生物化学和生物化学的结合来解决这些问题。
化学生物学一个合作网络支持这个项目。该项目的影响是提供(1)
合理靶向细胞包膜生物发生用于抗感染治疗的机会,以及(2)
需要研究分枝杆菌细胞包膜蛋白的工具。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria.
在快速和缓慢生长的分枝杆菌中优化 APEX2 过氧化物酶介导的邻近标记。
- DOI:10.1016/bs.mie.2021.11.021
- 发表时间:2022
- 期刊:
- 影响因子:0
- 作者:Ahamed,Mukshud;Jaisinghani,Neetika;Li,Michael;Winkeler,Ian;Silva,Shalika;Previti,MaryL;Seeliger,JessicaC
- 通讯作者:Seeliger,JessicaC
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jessica Chuang Seeliger其他文献
Jessica Chuang Seeliger的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jessica Chuang Seeliger', 18)}}的其他基金
Untangling the Inner Workings of the Mycobacterial Cell Envelope
解开分枝杆菌细胞膜的内部运作
- 批准号:
10004671 - 财政年份:2018
- 资助金额:
$ 39.88万 - 项目类别:
Untangling the Inner Workings of the Mycobacterial Cell Envelope
解开分枝杆菌细胞膜的内部运作
- 批准号:
10248369 - 财政年份:2018
- 资助金额:
$ 39.88万 - 项目类别:
Cell Envelope Biogenesis in Mycobacterium tuberculosis
结核分枝杆菌的细胞包膜生物发生
- 批准号:
10239074 - 财政年份:2018
- 资助金额:
$ 39.88万 - 项目类别:
Cell Envelope Biogenesis in Mycobacterium tuberculosis
结核分枝杆菌的细胞包膜生物发生
- 批准号:
10477002 - 财政年份:2018
- 资助金额:
$ 39.88万 - 项目类别:
Cell Envelope Biogenesis in Mycobacterium tuberculosis
结核分枝杆菌的细胞包膜生物发生
- 批准号:
9980785 - 财政年份:2018
- 资助金额:
$ 39.88万 - 项目类别:
Exploration of the Mycobacterial Cell Envelope Proteome by Protein Labeling in Live Cells
通过活细胞中的蛋白质标记探索分枝杆菌细胞包膜蛋白质组
- 批准号:
9284380 - 财政年份:2016
- 资助金额:
$ 39.88万 - 项目类别:
Exploration of the Mycobacterial Cell Envelope Proteome by Protein Labeling in Live Cells
通过活细胞中的蛋白质标记探索分枝杆菌细胞包膜蛋白质组
- 批准号:
9166299 - 财政年份:2016
- 资助金额:
$ 39.88万 - 项目类别:
相似海外基金
Sustainable routes to anti-infective agents to combat future pandemics.
对抗未来流行病的抗感染药物的可持续途径。
- 批准号:
2775420 - 财政年份:2022
- 资助金额:
$ 39.88万 - 项目类别:
Studentship
Antibiotic K16: Elucidation and Engineering Pathways to New Anti-infective Agents.
抗生素 K16:新型抗感染药物的阐明和工程途径。
- 批准号:
BB/V008552/1 - 财政年份:2021
- 资助金额:
$ 39.88万 - 项目类别:
Research Grant
Medicinal Chemistry Optimisation of Novel Heterocyclic Anti-infective Agents
新型杂环抗感染药物的药物化学优化
- 批准号:
2599532 - 财政年份:2021
- 资助金额:
$ 39.88万 - 项目类别:
Studentship
Pathways to New Anti-infective Agents
新型抗感染药物的开发途径
- 批准号:
2449001 - 财政年份:2020
- 资助金额:
$ 39.88万 - 项目类别:
Studentship
Admin Core - Autophagy Modulators as Novel Broad-Spectrum Anti-Infective Agents
管理核心 - 自噬调节剂作为新型广谱抗感染剂
- 批准号:
9893810 - 财政年份:2020
- 资助金额:
$ 39.88万 - 项目类别:
Autophagy Modulators as Novel Broad-Spectrum Anti-Infective Agents
自噬调节剂作为新型广谱抗感染剂
- 批准号:
10364721 - 财政年份:2019
- 资助金额:
$ 39.88万 - 项目类别:
Admin Core - Autophagy Modulators as Novel Broad-Spectrum Anti-Infective Agents
管理核心 - 自噬调节剂作为新型广谱抗感染剂
- 批准号:
10364722 - 财政年份:2019
- 资助金额:
$ 39.88万 - 项目类别:
Admin Core - Autophagy Modulators as Novel Broad-Spectrum Anti-Infective Agents
管理核心 - 自噬调节剂作为新型广谱抗感染剂
- 批准号:
10573255 - 财政年份:2019
- 资助金额:
$ 39.88万 - 项目类别:
Autophagy Modulators as Novel Broad-Spectrum Anti-Infective Agents
自噬调节剂作为新型广谱抗感染剂
- 批准号:
9893808 - 财政年份:2019
- 资助金额:
$ 39.88万 - 项目类别:
Autophagy Modulators as Novel Broad-Spectrum Anti-Infective Agents
自噬调节剂作为新型广谱抗感染剂
- 批准号:
10573254 - 财政年份:2019
- 资助金额:
$ 39.88万 - 项目类别: