Cracking post-translational modification codes in high molecular definition

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This project aims to markedly improve the analysis of post-translational modifications (PTM) via intact protein mass spectrometry. Differences in the PTM forms of a protein (modforms) can be crucial in many physiological and metabolic processes. However, current conventional methods cannot accurately separate nor fully assign most protein modforms. A recent discovery has resulted in the ability to separate whole protein ions that have the same mass, charge, and collision cross section, but subtly different charge sites. This project aims to leverage this breakthrough by developing novel approaches for separating intact protein modforms and mapping PTM sites. This is expected to be important for future biological discovery.