Rapid, ultra-sensitive protein structure elucidation by mass spectrometry

通过质谱法快速、超灵敏地阐明蛋白质结构

• 批准号: DP160102681
• 负责人: Prof William Donald
• 金额: $ 21万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160102681
• 关键词: Rapid; ultra; sensitive; protein; structure

This project seeks to develop a method for rapidly discovering classes of molecules that bind to unique sites on proteins. The development of new pharmaceuticals is frequently delayed by the time and resources required to identify the sites that new chemical entities bind to protein targets. A recent discovery has resulted in the ability to completely characterise large protein sequences directly from single mass spectra. This project aims to leverage this breakthrough by developing a rapid new approach for revealing ligand-protein binding sites using whole-protein mass spectrometry. This would enable novel sites of interactions between molecules and protein targets to be discovered rapidly with high sensitivity. In turn, this would allow the efficient design of next-generation classes of bioactive molecules.

该项目旨在开发一种快速发现与蛋白质上独特位点结合的分子类别的方法。新药的开发经常被鉴定新化学实体与蛋白质靶点结合的位点所需的时间和资源所延迟。最近的一项发现已经导致了直接从单个质谱中完全解析大蛋白质序列的能力。该项目旨在通过开发一种快速的新方法来利用全蛋白质质谱法揭示配体-蛋白质结合位点，从而利用这一突破。这将使分子和蛋白质靶点之间相互作用的新位点能够以高灵敏度快速发现。反过来，这将允许下一代生物活性分子的有效设计。