Ultrasensitive chemical analysis of single cells by mass spectrometry

通过质谱法对单细胞进行超灵敏化学分析

• 批准号: DP190103298
• 负责人: Prof William Donald
• 金额: $ 27.35万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2022-12-16
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190103298
• 关键词: Ultrasensitive; chemical; analysis; single; cells

This project aims to significantly improve the analysis of single cells by mass spectrometry with ultrahigh sensitivity. Molecular differences between cells in large populations can be important in many processes, including cell differentiation and signalling. However, most measurements result in the chemical heterogeneity between cells being masked by the bulk population. Recent discoveries now make it feasible to measure thousands of proteins and small molecules from single cells. This project aims to leverage these breakthroughs to allow heterogeneity to be characterised in single cells with unprecedented molecular detail. This is expected to be important for future biological discovery, as well as disease prediction and drug discovery.

该项目旨在通过质谱法显著提高单细胞分析的灵敏度。大群体中细胞之间的分子差异在许多过程中可能很重要，包括细胞分化和信号传导。然而，大多数测量导致细胞之间的化学异质性被大量群体掩盖。最近的发现使从单细胞中测量数千种蛋白质和小分子成为可能。该项目旨在利用这些突破，以前所未有的分子细节在单细胞中表征异质性。预计这对未来的生物发现以及疾病预测和药物发现都很重要。