ONIX: A Neural Acquisition System for Unencumbered, Closed-Loop Recordings in Small, Freely Moving Animals

ONIX：一种神经采集系统，用于对小型、自由移动的动物进行无阻碍、闭环记录

• 批准号: 10482182
• 负责人: Jonathan Newman
• 金额: $ 70.72万
• 依托单位: OPEN EPHYS, INC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-23 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10482182
• 关键词: 3-Dimensional; 3D Print; Action Potentials; Adopted; Age; Amplifiers; Animal Behavior; Animals; Behavior; Behavioral; Brain; Calcium; Communication; Complex; Computer software; Country; Custom; Data Compression; Development; Devices; Eastern Cooperative Oncology Group; Ecosystem; Electrodes; Electroencephalography; Electronics; Electrophysiology (science); Endoscopes; Engineering; Environment; Feedback; Financial compensation; Goals; Grooming; Head; Hour; Image; Implant; Injections; Learning; Light; Manuals; Measurement; Measures; Miniaturization; Modernization; Molds; Motion; Movement; Mus; Nature; Needles; Neurons; Neurosciences; Nobel Prize; Optics; Partner in relationship; Penetration; Performance; Phase; Polymorph; Positioning Attribute; Price; Production; Protocols documentation; Recording of previous events; Research; Robot; Rotation; Running; Site; Sleep; Small Business Innovation Research Grant; Software Design; Speed; System; Technology; Testing; Time; Torque; Tube; Vacuum; Vision; Weight; Work; commercialization; cost; design; digital; experimental study; extracellular; flexibility; head mounted display; innovation; interest; kinematics; microdevice; neural implant; neuroethology; next generation; online resource; open source; prototype; relating to nervous system; tool; usability

PROJECT SUMMARY Over the last century, extracellular recording technologies have progressed from handmade needle electrodes and vacuum tube amplifiers to microfabricated devices containing hundreds of recording sites and on-chip digitization circuits. This technological arc has facilitated Nobel-prize winning discoveries such as the unitary nature of action potentials, the computations underlying mammalian vision, and the neural basis of spatial learning, among many other notable findings. To study increasingly sophisticated aspects of brain function, technical development has been driven by two competing goals: (1) permitting high-fidelity communication with as many neurons as possible and (2) minimizing the impact of recordings on normal animal behavior. While great progress has been made on the first goal, there is growing recognition that increased effort must be allotted to reduce the behavioral burden of neural implants, especially in mice. Toward this end, Open Ephys Inc. proposes to create ONIX, a high-performance neural recording ecosystem that permits unencumbered mouse behavior for arbitrarily long recording sessions in large, complex environments. A key component of ONIX will be head-mounted devices that measure their own position and orientation. This information is sent to a low-cost, easy-to-install cable robot that keeps the ONIX ultra-light tether approximately vertical at all times while compensating for tether induced head torque. This enables, for the first time, recordings during unencumbered naturalistic mouse behavior. Further, using advanced digital circuitry, ONIX will be probe-agnostic and capable of working with all modern recording devices, including Intan chips, Neuropixels, and miniature endoscopes for calcium imaging. ONIX will seamlessly integrate into existing, widely used software such as the Open Ephys GUI, Bonsai, and DeepLabCut Live for maximum market penetration without requiring labs to learn new tools or modify acquisition workflows. In summary, ONIX will provide high-bandwidth, closed-loop performance without burdening mice in room-scale 3D environments. In combination with other innovative tools for rich, quantitative measurement of animal behavior, ONIX will accelerate the field towards a golden age of quantitative neuroethology.

项目摘要 在上个世纪，细胞外记录技术已经从手工针电极 和真空管放大器到包含数百个记录点和片上的微制造设备 数字化电路这一技术弧促进了诺贝尔奖的发现，如单一的 动作电位的性质，哺乳动物视觉的基础计算，以及空间视觉的神经基础 学习，以及其他许多值得注意的发现。为了研究日益复杂的大脑功能， 技术的发展是由两个相互竞争的目标驱动的：（1）允许高保真通信， 尽可能多的神经元和（2）最小化记录对正常动物行为的影响。而 虽然在第一个目标上取得了很大进展，但人们越来越认识到，必须加大努力， 分配用于减少神经植入物的行为负担，特别是在小鼠中。 为此，Open Ephys Inc.建议创建ONIX，一个高性能的神经记录生态系统 允许不受限制的鼠标行为，用于任意长的记录会话， 环境. ONIX的一个关键组成部分将是头戴式设备，测量自己的位置， 导向这些信息被发送到一个低成本、易于安装的电缆机器人，使ONIX超轻 在补偿系绳引起的头部扭矩的同时，始终保持系绳大致垂直。这使得，对于 第一次，在不受阻碍的自然主义小鼠行为的记录。此外，使用先进的数字 电路，ONIX将探针不可知，并能够与所有现代记录设备，包括Intan 芯片、神经像素和用于钙成像的微型内窥镜。ONIX将无缝集成到 现有的，广泛使用的软件，如Open Ephys GUI，Bonsai和DeepLabCut Live， 市场渗透，而无需实验室学习新工具或修改采购工作流程。总之，ONIX 将提供高带宽、闭环性能，而不会在房间规模的3D环境中增加鼠标的负担。 结合其他创新工具，ONIX将对动物行为进行丰富的定量测量， 加速该领域走向定量神经行为学的黄金时代。