Noncanonical functions of the ribosome in response to cellular stress

核糖体响应细胞应激的非常规功能

• 批准号: 10487140
• 负责人: Colin Wu
• 金额: $ 111.01万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10487140
• 关键词: Apoptotic; Biochemical; CRISPR screen; Cell Death; Cells; Cellular Stress; Cessation of life; Chemicals; Computing Methodologies; Cues; Data; Distress; Gene Expression; Goals; Health; Human; Hypoxia; Laboratories; Life; Mass Spectrum Analysis; Messenger RNA; Modification; Molecular; Monitor; Nutrient; Output; RNA; Research; Ribosomes; Starvation; Stress; Techniques; Technology; Translational Regulation; Work; genome-wide; member; nanopore; novel; programs; response; ribosome profiling; ultraviolet irradiation

My laboratory broadly investigates the function of the ribosome in determining cell fate decisions. Some work began soon after the launch of the lab in late 2020, the setup of the laboratory space was accomplished, and section members were hired. Research in the lab has focused on how ribosomes sense and respond to translational distress induced by cellular or environmental stress conditions, such as nutrient starvation, hypoxia, and UV irradiation. Cellular stress influences how ribosomes move along the mRNA templates, therefore changing the translational landscape and ultimately altering the gene expression programs of the cell. A better understanding of the mechanism of translational regulation under stress conditions is therefore essential for overcoming challenges to human health. We have begun deciphering the molecular mechanisms of cell death induced by damaged RNA. We have adapted Nanopore RNA direct sequencing technology to monitor RNA damage and modification caused by cellular and environmental stress, and we are currently developing computational methods for analyzing Nanopore sequencing data from stressed cells.

我的实验室广泛研究核糖体在决定细胞命运中的作用。在2020年底实验室发射后不久，一些工作就开始了，实验室空间的设置完成了，并聘请了科员。实验室的研究重点是核糖体如何感知和响应由细胞或环境应激条件引起的翻译困难，如营养饥饿、低氧和紫外线照射。细胞应激影响核糖体如何沿着mRNA模板移动，从而改变翻译格局，并最终改变细胞的基因表达程序。因此，更好地理解应激条件下的翻译调控机制对于克服对人类健康的挑战至关重要。我们已经开始破译RNA损伤导致细胞死亡的分子机制。我们已经采用了纳米孔RNA直接测序技术来监测细胞和环境应激引起的RNA损伤和修改，目前我们正在开发分析应激细胞的纳米孔测序数据的计算方法。