Noncanonical functions of the ribosome in response to cellular stress

我的实验室广泛研究核糖体在决定细胞命运方面的功能。实验室于2020年底启动后不久就开始了一些工作，完成了实验室空间的设置，并聘请了部门成员。实验室的研究主要集中在核糖体如何感知和响应由细胞或环境应激条件（如营养饥饿、缺氧和紫外线照射）引起的翻译窘迫。细胞应激影响核糖体如何沿着mRNA模板移动，因此改变了翻译景观并最终改变了细胞的基因表达程序。因此，更好地了解应激条件下的翻译调节机制对于克服对人类健康的挑战至关重要。我们已经开始破译受损RNA诱导细胞死亡的分子机制。我们已经采用纳米孔RNA直接测序技术来监测细胞和环境应激引起的RNA损伤和修饰，我们目前正在开发计算方法来分析来自应激细胞的纳米孔测序数据。

My laboratory broadly investigates the function of the ribosome in determining cell fate decisions. Some work began soon after the launch of the lab in late 2020, the setup of the laboratory space was accomplished, and section members were hired. Research in the lab has focused on how ribosomes sense and respond to translational distress induced by cellular or environmental stress conditions, such as nutrient starvation, hypoxia, and UV irradiation. Cellular stress influences how ribosomes move along the mRNA templates, therefore changing the translational landscape and ultimately altering the gene expression programs of the cell. A better understanding of the mechanism of translational regulation under stress conditions is therefore essential for overcoming challenges to human health. We have begun deciphering the molecular mechanisms of cell death induced by damaged RNA. We have adapted Nanopore RNA direct sequencing technology to monitor RNA damage and modification caused by cellular and environmental stress, and we are currently developing computational methods for analyzing Nanopore sequencing data from stressed cells.