Sequence determinants of membraneless organelle rheology -- Research supplement to promote diversity

无膜细胞器流变学的序列决定因素——促进多样性的研究补充

• 批准号: 10491523
• 负责人: Benjamin S Schuster
• 金额: $ 4.21万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10491523
• 关键词: 2019-nCoV; Affect; Amino Acid Sequence; Award; Biological Process; Biomedical Engineering; Biomedical Research; Communities; Complement; Disease; Doctor of Philosophy; Fostering; Funding; Goals; Grant; Health; Hispanic; Institution; Membrane; Membrane Proteins; Motivation; Nucleocapsid Proteins; Organelles; Parents; Phase; Process; Proteins; Research; Rheology; Training; Underrepresented Minority; Universities; Viral; Virus; Virus Assembly; Virus Replication; career; doctoral student; experience; falls; high school; parent grant; role model

PROJECT SUMMARY/ABSTRACT This is an application for a Research Supplement to Promote Diversity in Health-Related Research to complement our parent grant, “Sequence determinants of membraneless organelle rheology” (R35 GM142903). The supplement funds will support Bruna Favetta, who in Fall 2021 is starting her second year as a Ph.D. student in the Department of Biomedical Engineering at Rutgers University. Bruna has excellent background, motivation, and ability to contribute to the overall goals of the parent grant. The objective of the parent grant is to understand how the sequences of intrinsically disordered proteins (IDPs) contribute to the rheology of membraneless organelles (also known as biomolecular condensates), and ultimately how that rheology relates to function. Bruna is Hispanic and is committed to serving as a role model for underrepresented minorities in biomedical research, both at Rutgers and in her future research career. Bruna has conducted research at several universities and institutions beginning when she was only in high school, giving her a unique perspective on research in diverse settings around the globe. Bruna brings these experiences to advance her own research at Rutgers and to enrich the overall scientific community through fostering greater understanding of diversity in research. Together, we have crafted a plan for Bruna’s training and research during her Ph.D. studies. Bruna will study the rheology of biomolecular condensates formed from the IDPs of the SARS-CoV-2 virus. These proteins phase separate into biomolecular condensates during the viral replication lifecycle, and we hypothesize that the rheology of these condensates has important functional consequences for viral assembly, including membrane scission. The first goal is to quantify the rheology of the multiphasic condensates composed of the nucleocapsid (N) and membrane (M) proteins of SARS-CoV-2. Next, we will assess how perturbations to the rheology of these viral condensates may affect their biological function, such as orchestrating the important processes of membrane bending and viral membrane scission. Overall, this research will foster Bruna’s training and will advance our understanding of how IDP sequence determines biomolecular condensate rheology and how this rheology gives rise to biological function, which is the overarching focus of the parent R35 grant.

项目总结/摘要 这是一份促进健康相关研究多样性的研究补充申请， 补充我们的父母补助金，“无膜细胞器流变学的序列决定因素”（R35 GM 142903）。补充资金将支持Bruna Favetta，她将于2021年秋季开始她作为 博士学位罗格斯大学生物医学工程系的学生。布鲁纳拥有出色的 背景，动机和能力，以促进家长补助金的总体目标。的目的 父母补助金是为了了解如何内在无序蛋白质（IDP）的序列有助于 无膜细胞器（也称为生物分子凝聚物）的流变学，以及最终如何 流变学与功能有关。布鲁纳是西班牙裔，并致力于作为一个榜样， 少数民族在生物医学研究中的代表性不足，无论是在罗格斯大学还是在她未来的研究生涯中。布鲁娜 从高中开始，她就在几所大学和机构进行研究， 这使她对地球仪不同环境的研究有了独特的视角。布鲁娜带来了这些 经验，以推进她自己的研究在罗格斯大学，并通过丰富整个科学界 促进对研究多样性的更好理解。我们一起为布鲁娜的训练制定了一个计划 在她的博士学位期间进行研究。问题研究布鲁纳将研究生物分子凝聚物的流变学， SARS-CoV-2病毒的国内流离失所者。这些蛋白质相分离成生物分子冷凝物， 病毒复制生命周期，我们假设这些冷凝物的流变学具有重要的功能 病毒装配的后果，包括膜断裂。第一个目标是量化的流变学的 由SARS-CoV-2的核衣壳（N）和膜（M）蛋白组成的多相缩合物。 接下来，我们将评估这些病毒浓缩物的流变学扰动如何影响其生物学特性。 功能，如协调膜弯曲和病毒膜断裂的重要过程。 总的来说，这项研究将促进布鲁纳的培训，并将促进我们对IDP序列如何 决定了生物分子凝聚物的流变性，以及这种流变性如何产生生物功能， 父R35补助金的首要重点。