Sequence determinants of membraneless organelle rheology -- Research supplement to promote diversity

项目摘要/摘要 这是一份促进健康相关研究多样性的研究补充材料的申请书 补充我们的母基金，“无膜细胞器流变学的序列决定因素”(R35 GM142903)。补充基金将支持Bruna Favetta，她将于2021年秋季开始她作为 罗格斯大学生物医学工程系的博士生。布鲁纳有很好的 背景、动机和能力，为家长奖助金的总体目标做出贡献。该计划的目标是 Parent Grant是为了了解内在无序蛋白(IDPs)的序列是如何对 无膜细胞器的流变学(也称为生物分子凝聚体)，以及最终如何 流变学与功能有关。布鲁纳是西班牙裔，致力于成为 在生物医学研究中，无论是在罗格斯大学还是在她未来的研究生涯中，都没有充分代表少数族裔。布鲁纳 从她上高中的时候就开始在几所大学和机构进行研究， 使她对全球不同环境下的研究有了独特的视角。布鲁娜带来了这些 在罗格斯推进自己的研究并通过以下方式丰富整个科学界的经历 在研究中促进对多样性的更多理解。我们一起为布鲁娜的训练制定了一个计划 并在攻读博士学位期间进行研究。布鲁纳将研究生物分子凝析油的流变学 SARS-CoV-2病毒的国内流离失所者。这些蛋白质在过程中分离成生物分子缩合物 病毒复制生命周期，我们假设这些冷凝物的流变性具有重要的功能 病毒组装的后果，包括膜破裂。第一个目标是量化材料的流变性 由SARS-CoV-2核衣壳蛋白(N)和膜蛋白(M)组成的多相凝聚体。 接下来，我们将评估这些病毒凝聚体的流变性扰动可能会如何影响它们的生物 功能，如协调膜弯曲和病毒膜断裂的重要过程。 总体而言，这项研究将促进Bruna的训练，并将促进我们对IDP序列的理解 决定生物分子凝析油的流变性以及这种流变性如何产生生物功能，这是 母公司R35赠款的主要重点。

PROJECT SUMMARY/ABSTRACT This is an application for a Research Supplement to Promote Diversity in Health-Related Research to complement our parent grant, “Sequence determinants of membraneless organelle rheology” (R35 GM142903). The supplement funds will support Bruna Favetta, who in Fall 2021 is starting her second year as a Ph.D. student in the Department of Biomedical Engineering at Rutgers University. Bruna has excellent background, motivation, and ability to contribute to the overall goals of the parent grant. The objective of the parent grant is to understand how the sequences of intrinsically disordered proteins (IDPs) contribute to the rheology of membraneless organelles (also known as biomolecular condensates), and ultimately how that rheology relates to function. Bruna is Hispanic and is committed to serving as a role model for underrepresented minorities in biomedical research, both at Rutgers and in her future research career. Bruna has conducted research at several universities and institutions beginning when she was only in high school, giving her a unique perspective on research in diverse settings around the globe. Bruna brings these experiences to advance her own research at Rutgers and to enrich the overall scientific community through fostering greater understanding of diversity in research. Together, we have crafted a plan for Bruna’s training and research during her Ph.D. studies. Bruna will study the rheology of biomolecular condensates formed from the IDPs of the SARS-CoV-2 virus. These proteins phase separate into biomolecular condensates during the viral replication lifecycle, and we hypothesize that the rheology of these condensates has important functional consequences for viral assembly, including membrane scission. The first goal is to quantify the rheology of the multiphasic condensates composed of the nucleocapsid (N) and membrane (M) proteins of SARS-CoV-2. Next, we will assess how perturbations to the rheology of these viral condensates may affect their biological function, such as orchestrating the important processes of membrane bending and viral membrane scission. Overall, this research will foster Bruna’s training and will advance our understanding of how IDP sequence determines biomolecular condensate rheology and how this rheology gives rise to biological function, which is the overarching focus of the parent R35 grant.