Fluorescence Microscopy and Cell Imaging

项目摘要/摘要-荧光显微镜与细胞成像共享资源 联合国儿童基金会荧光显微镜和细胞成像共享资源(显微镜)支持基本和 通过向联合国儿童基金会成员和非成员用户提供用于定量、多尺度和多模式荧光成像的最先进的仪器和软件，进行转化性癌症研究。教务主任 Diane Lidke博士(CMO)负责管理由全职Michael Paffett博士运营的资源 在荧光显微镜仪器方面拥有广泛专业知识的员工。联合国排雷行动中心研究局 管理资源，该资源提供对11个商业仪器的访问，用于荧光、相或 Brightfield成像，以及用于图像分析的多种软件平台。此外，CCC成员还拥有 访问超分辨率核心，由Keith Lidke博士(CMO)指导，包含尖端和 用于单分子、超分辨率和高光谱成像的开发仪器。超分辨率核心得到了迈克尔·韦斯特博士的进一步支持，他是单分子图像方面的专家 分析。这一伙伴关系为联合国儿童基金会成员提供了其他癌症中心无法获得的技术 以及开发新的成像和分析方法的机会，以专门解决突出的问题 癌症生物学中的问题。购买、维护和运营所需的费用和重要的专业知识 如此多样化的工具套件，对于大多数用户来说是令人望而却步的，并强调了 资源。显微镜积极促进和传播有关新细胞成像技术的信息 维持最新的网页，并在联东综合团的会议和务虚会上作专题介绍。 资源教职员工还为多项教育活动做出贡献，包括侧重于 吸引联合国儿童基金会成员实验室的研究生和博士后的显微技术。预订、帐单和 使用情况通过一个集中的在线系统进行跟踪。在上一个五年期项目期间，59个中乍特派团 联合国儿童基金会三个研究方案的成员实验室使用了该资源，出版了67份出版物。 在前一期间，通过记账和支付费用，联塞特派团成员占总资源使用量的61% 得到了132个同行审查赠款和8个联合国儿童基金会试点项目的支持。

Project Summary/Abstract - FLUORESCENCE MICROSCOPY & CELL IMAGING SHARED RESOURCE The UNMCCC Fluorescence Microscopy & Cell Imaging Shared Resource (Microscopy) supports basic and translational cancer research by providing state-of-the-art instrumentation and software for quantitative, multiscale, and multi-modal fluorescence imaging, to UNMCCC members and non-member users. Faculty Director Diane Lidke, Ph.D. (CMO) supervises the Resource, which is operated by Michael Paffett, Ph.D., a full-time staff with broad expertise in fluorescence microscopy instrumentation. The UNMCCC Research Administration manages the Resource, which provides access to 11 commercial instruments for fluorescence, phase or brightfield imaging, as well as multiple software platforms for image analysis. In addition, CCC members have access to the Super-Resolution Core, directed by Keith Lidke, Ph.D. (CMO) that houses cutting-edge and developmental instrumentation for single molecule, super-resolution and hyperspectral imaging. The SuperResolution core is further supported by Michael Wester, Ph.D. who is an expert in single molecule image analysis. This partnership provides UNMCCC members with technologies not available at other Cancer Centers and the opportunity to develop new imaging and analysis approaches to specifically address outstanding questions in cancer biology. The expense and significant expertise required to purchase, maintain and operate such a diverse suite of instrumentation, is prohibitive for most users and underscores the value added by the Resource. Microscopy actively facilitates and disseminates information about new cellular imaging technologies by maintaining an up-to-date web page and by giving presentations at UNMCCC meetings and retreats. Resource faculty and staff also contribute to multiple education activities, including courses focused on microscopy that attract graduate students and postdocs from UNMCCC member labs. Reservations, billing and usage are tracked through a centralized on-line system. During the previous 5-year project period, 59 UNMCCC member laboratories from three UNMCCC Research Programs used the Resource, resulting in 67 publications. In the previous period, UNMCCC members were responsible for 61% of total Resource usage by billing and were supported by 132 peer-reviewed grants and 8 UNMCCC pilot projects.