Fluorescence Microscopy and Cell Imaging

荧光显微镜和细胞成像

• 批准号: 10491181
• 负责人: Diane Lidke
• 金额: $ 9.26万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-26 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10491181
• 关键词: 3-Dimensional; Address; Algorithmic Analysis; Award; Biological Assay; Biophysics; Cancer Biology; Cancer Center; Cancer Center Support Grant; Catchment Area; Cell physiology; Cells; Cellular biology; Collaborations; Color; Comprehensive Cancer Center; Computer software; Consultations; Development; Doctor of Philosophy; Education; Ensure; Equipment; Faculty; Feedback; Fluorescence; Fluorescence Microscopy; Future; Goals; Grant; Human Resources; Image; Image Analysis; Imaging Techniques; Imaging technology; Investigation; Laboratories; Malignant - descriptor; Methods; Microscope; Microscopy; Molecular and Cellular Biology; NCI Center for Cancer Research; New Mexico; Newsletter; Oncogenic; Online Systems; Peer Review Grants; Phase; Pilot Projects; Postdoctoral Fellow; Preparation; Publications; Research; Research Personnel; Reservations; Resistance; Resolution; Resource Sharing; Resources; Sampling; Schedule; Services; Signal Transduction; Solvents; Supervision; Surveys; System; Techniques; Technology; Time; Tissue imaging; Tissues; Training; Universities; University resources; Update; Visualization; anticancer research; assay development; cancer cell; cell behavior; cellular imaging; cost effective; cost effectiveness; experience; fluorescence imaging; graduate student; imaging capabilities; imaging modality; imaging platform; innovation; instrument; instrumentation; lectures; light microscopy; meetings; member; microscopic imaging; molecular imaging; multimodality; nanoscale; new technology; novel; particle; programs; prospective; protein protein interaction; quantitative imaging; single molecule; technology development; temporal measurement; translational cancer research; web page; web site

Project Summary/Abstract - FLUORESCENCE MICROSCOPY & CELL IMAGING SHARED RESOURCE The UNMCCC Fluorescence Microscopy & Cell Imaging Shared Resource (Microscopy) supports basic and translational cancer research by providing state-of-the-art instrumentation and software for quantitative, multiscale, and multi-modal fluorescence imaging, to UNMCCC members and non-member users. Faculty Director Diane Lidke, Ph.D. (CMO) supervises the Resource, which is operated by Michael Paffett, Ph.D., a full-time staff with broad expertise in fluorescence microscopy instrumentation. The UNMCCC Research Administration manages the Resource, which provides access to 11 commercial instruments for fluorescence, phase or brightfield imaging, as well as multiple software platforms for image analysis. In addition, CCC members have access to the Super-Resolution Core, directed by Keith Lidke, Ph.D. (CMO) that houses cutting-edge and developmental instrumentation for single molecule, super-resolution and hyperspectral imaging. The SuperResolution core is further supported by Michael Wester, Ph.D. who is an expert in single molecule image analysis. This partnership provides UNMCCC members with technologies not available at other Cancer Centers and the opportunity to develop new imaging and analysis approaches to specifically address outstanding questions in cancer biology. The expense and significant expertise required to purchase, maintain and operate such a diverse suite of instrumentation, is prohibitive for most users and underscores the value added by the Resource. Microscopy actively facilitates and disseminates information about new cellular imaging technologies by maintaining an up-to-date web page and by giving presentations at UNMCCC meetings and retreats. Resource faculty and staff also contribute to multiple education activities, including courses focused on microscopy that attract graduate students and postdocs from UNMCCC member labs. Reservations, billing and usage are tracked through a centralized on-line system. During the previous 5-year project period, 59 UNMCCC member laboratories from three UNMCCC Research Programs used the Resource, resulting in 67 publications. In the previous period, UNMCCC members were responsible for 61% of total Resource usage by billing and were supported by 132 peer-reviewed grants and 8 UNMCCC pilot projects.

项目概要/摘要-荧光显微镜和细胞成像共享资源 UNMCCC荧光显微镜和细胞成像共享资源（显微镜）支持基本和 通过提供最先进的仪器和软件进行定量，多靶点，向联合国气候变化协调中心成员和非成员用户提供了一个新的、可扩展的、可扩展的、多模式的荧光成像系统。课程主任 Diane Lidke博士(CMO)监督由Michael Paffett博士运营的资源，全职 在荧光显微镜仪器方面具有广泛专业知识的工作人员。UNCCC研究管理局 管理资源，提供11个商业仪器的荧光，相位或 明场成像以及用于图像分析的多个软件平台。此外，CCC成员还 访问由基思利德克博士指导的超分辨率核心。(CMO)拥有尖端技术， 用于单分子、超分辨率和高光谱成像的开发仪器。超级分辨率核心由Michael Wester博士进一步支持。他是单分子成像的专家 分析.这种伙伴关系为联合国癌症中心成员提供了其他癌症中心无法提供的技术 并有机会开发新的成像和分析方法，以专门解决突出的 癌症生物学的问题。购买、维护和运营所需的费用和重要的专业知识 这样一套多样化的仪器，对大多数用户来说是禁止的，并强调了 Resource.显微镜积极促进和传播有关新的细胞成像技术的信息 通过保持一个最新网页和在联合国儿童基金会会议和务虚会上发言， 资源教师和工作人员也有助于多种教育活动，包括课程的重点是 显微镜，吸引研究生和博士后从UNMCCC成员实验室。预订、计费和 通过一个集中的在线系统跟踪使用情况。在上一个五年项目期间， 来自联合国气候变化协调中心三个研究方案的成员实验室使用了该资源，出版了67份出版物。 在上一个期间，联合国儿童基金会成员按账单计算占资源使用总额的61%， 得到了132项同行审查赠款和8个联合国儿童基金会试点项目的支助。