Human Tissue Repository and Tissue Analysis

项目摘要/摘要-人体组织信息库和组织分析共享资源 联合国儿童基金会人体组织储存库和组织分析共享资源(HTR组织)，支持基本 以及通过提供成本效益高的肿瘤和匹配的正常组织作为冰冻的 或福尔马林固定、石蜡包埋(FFPE)等份，提供给联塞特派团成员和非成员用户。科学 总监Dennis J.McCance，Ph.D.(CT)监督由全职工作人员操作的资源 包括经验丰富的湿实验室研究技术人员以及一名提供符合以下要求的服务的数字成像专家 美国病理学家学院(CAP)认证计划。联东综合团和新喀彻温省人权委员会 病理科共同管理资源，提供几项基本服务，包括 组织切片、HE、免疫组织化学或免疫荧光染色及数字成像 以及染色标本的注释。该资源目前容纳了40,000个生物标本，其中20,000个 冷冻和13,000份FFPE生物检材和6,000份血液样本(全血、血清、血浆和两者 冷冻和冷冻保存的细胞)，反映了我们新墨西哥州人口的种族/民族，36%的 样本来自西班牙裔，49%来自非西班牙裔白人，12%来自美洲原住民，3%来自其他 种族/民族。在上次拨款期间，我们发放了1,196份组织样本，1,496张用于DNA/RNA的载片 提取和加工了4000个动物组织。由于癌症中心已经开发出精准医学 倡议并加入了Orien财团，HTR组织扩大了组织收集范围，包括血液 和/或来自所有同意的患者的唾液(生殖系对照)，以及来自 恶性血液病(新服务)。该资源维护所收集的组织的数据库 (TisseMetrix)，包含有关患者的关键信息(如姓名、出生日期和种族/民族)、组织来源 和诊断，以及其他变量包括组织收集的方法，缺血时间，以及组织如何 在HTR组织收到后进行处理。项目、账单和使用情况通过一个集中的在线系统进行跟踪。资源总监和员工与研究计划密切合作，开发新的 积极促进创新的基础和转化性研究目标和资源的方法 通过维护最新网页和在联东综合团会议和务虚会上发言，促进和传播有关新的成像和注释技术的信息。在之前的5年内 在项目期间，101名调查员，其中59人是来自所有3个特派团研究方案的特派团成员， 已使用资源。该资源为联东综合团用户出版的38份以癌症为重点的出版物提供了帮助， 得到了120个同行评审赠款的支持。在2019年7月-2020年6月的报告年度，CCSG 支助17%的资源预算，联东综合团成员负责74%的资源使用。

Project Summary/Abstract - Human Tissue Repository & Tissue Analysis Shared Resource The UNMCCC Human Tissue Repository and Tissue Analysis Shared Resource (HTR Tissue), supports basic and translational cancer research by providing cost effective tumor and matched normal tissue either as frozen or Formalin-Fixed, Paraffin-Embedded (FFPE) aliquots to UNMCCC members and non-member users. Scientific Director Dennis J. McCance, Ph.D. (CT) supervises the Resource, which is operated by a full-time staff including experienced wet lab research technicians plus a digital imaging expert providing services aligned with the College of American Pathologist (CAP) Accreditation Program. The UNMCCC and the UNM HSC Department of Pathology jointly manage the Resource, which provides several essential services including sectioning, H&E, immunohistochemical or immunofluorescence staining of tissue sections and digital imaging and annotation of stained specimens. The Resource currently houses > 40,000 biospecimens, with >20,000 frozen and >13,000 FFPE biospecimens and >6,000 blood samples (whole blood, serum, plasma and both frozen and cryo-preserved cells) that reflect the race/ethnicity of our population in New Mexico with 36% of the samples from Hispanic, 49% from non-Hispanic white and 12% from Native American and 3% other race/ethnicities. During the last grant period we dispensed 1,196 tissue specimens, 1,496 slides for DNA/RNA extraction and processed 4,000 animal tissues. As the Cancer Center has developed its precision medicine initiative and joined the ORIEN consortium, HTR Tissue has expanded the tissue collection to include blood and/or saliva from all consented patients (germline control), and blood and bone marrow from patients with hematological malignancies (New Services). The Resource maintains a database of the collected tissues (TissueMetrix) with critical information on the patient (e.g. name, date of birth and race/ethnicity), tissue source and diagnosis, and other variables including the method of tissue collection, ischemic times, and how the tissue was processed after receipt by the HTR Tissue. Projects, billing and usage are tracked through a centralized online system. The Resource Director and staff work closely with the Research Programs to develop new methodologies that promote innovative basic and translational research goals and the Resource actively facilitates and disseminates information about new imaging and annotation technologies by maintaining an upto-date web page and by giving presentations at UNMCCC meetings and retreats. During the previous 5-year project period, 101 investigators, of whom 59 were UNMCCC members from all 3 UNMCCC Research Programs, used the Resource. The Resource contributed to 38 cancer focused publications by the UNMCCC users, who were supported by 120 peer-reviewed grants. In the reporting year of July 2019 – June 2020, the CCSG supported 17% of the Resource budget with UNMCCC members responsible for 74% of Resource usage.